The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Function of AP-1 in Transcription of the Telomerase Reverse Transcriptase Gene (TERT) in Human and Mouse Cells
Open accessAbstract
The transcriptional regulation of the human telomerase catalytic subunit (hTERT) plays a critical role in telomerase activity. Approximately 200 bp of the proximal core promoter is responsible for basic hTERT expression; however, the function of the distal regulatory elements remains unclear. The transcription factor activator protein 1 (AP-1) is involved in cellular proliferation, differentiation, carcinogenesis, and apoptosis and is expressed broadly in both cancer and normal cells. There are several putative AP-1 sites in the hTERT promoter, but their functions are unknown. The present study examined the regulatory role of AP-1 in hTERT gene transcription. Overexpression of AP-1 leads to transcriptional suppression of hTERT in cancer cells. The combination of c-Fos and c-Jun or c-Fos and JunD strongly suppresses hTERT promoter activity in transient-expression analyses. The hTERT promoter region between -2000 and -378 is responsible for this function. Gel shift and supershift analyses, as well as ChIP, show binding of JunD and c-Jun on two putative AP-1 sites within this region. Mutations in the AP-1 binding sites rescued suppressions caused by AP-1, suggesting this is a direct regulation of the hTERT promoter. In contrast, there was no effect on mTERT expression or mTERT promoter activity by AP-1 overexpression in mouse fibroblasts. The species-specific function of AP-1 in TERT expression may in part help explain the difference in telomerase activity between normal human and mouse cells.
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“Of particular interest is the observation that AP-1 had no effect on the mouse TERT (mTERT ) promoter although it has similar binding sites for AP-1.”
From Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers (2008) · cites this paper as [28]
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Specific Association of Human Telomerase Activity with Immortal Cells and Cancer
Kim, N. W., Piatyszek, M. A., Prowse, K. R., Harley, C. B., West, M. D., Ho, P. L. C., Coviello, G. M., Wright, W. E., Weinrich, S. L., & Shay, J. W. (1994) · Science · doi:10.1126/science.7605428 · Cited by 7,091 · cited 1×
“Detectable telomerase activity is observed only in some highly regenerative tissues such as hematopoietic progenitor, intestinal crypt, skin basal layer, cervical keratinocyte, endometrium, and germ line cells; however, 75 to 85% of cancer cells show telomerase activity (9, 13, 16, 21 , 23, 25, 37, 43).”
BackgroundSelf-citationTelomeres shorten during ageing of human fibroblasts
Harley, C. B., Futcher, A. B., & Greider, C. W. (1990) · Nature · doi:10.1038/345458a0 · Cited by 5,750
Extension of Life-Span by Introduction of Telomerase into Normal Human Cells
Bodnar, A. G., Ouellette, M., Frolkis, M., Holt, S. E., Chiu, C.-P., Morin, G. B., Harley, C. B., Shay, J. W., Lichtsteiner, S., & Wright, W. E. (1998) · Science · doi:10.1126/science.279.5349.349 · Cited by 4,975
Self-citationSchreiber, E. H., Matthias, P., Müller, M. M., & Schaffner, W. (1989) · Nucleic Acids Research · doi:10.1093/nar/17.15.6419 · Cited by 4,179
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
AP-1 as a regulator of cell life and death
Shaulian, E., & Karin, M. (2002) · Nature Cell Biology · doi:10.1038/ncb0502-e131 · Cited by 2,961
A survey of telomerase activity in human cancer
Shay, J. W., & Bacchetti, S. (1997) · European Journal of Cancer · doi:10.1016/s0959-8049(97)00062-2 · Cited by 2,794
Self-citationTelomerase Catalytic Subunit Homologs from Fission Yeast and Human
Nakamura, T. M., Morin, G. B., Chapman, K. B., Weinrich, S. L., Andrews, W. H., Lingner, J., Harley, C. B., & Cech, T. R. (1997) · Science · doi:10.1126/science.277.5328.955 · Cited by 2,256
The RNA Component of Human Telomerase
Feng, J., Funk, W. D., Wang, S.-S., Weinrich, S. L., Avilion, A. A., Chiu, C.-P., Adams, R. R., Chang, E., Allsopp, R. C., Yu, J., et al. (1995) · Science · doi:10.1126/science.7544491 · Cited by 2,227
Flagged first, then by citations
Research works printed but not linked: 10
- Alfonso-De Matte M. Y., 2002, Cancer Res., 62, 4575
- Bushel P., 1995, Oncogene, 10, 1361
- Devereux T. R., 1999, Cancer Res., 59, 6087
- Hirai S., 1990, Oncogene, 5, 39
- Horikawa I., 1999, Cancer Res., 59, 826
- Ito H., 1998, Clin. Cancer Res., 4, 1603
- Kyo S., 1997, Cancer Res., 57, 610
- Takakura M., 1999, Cancer Res., 59, 551
- Takakura M., 1998, Cancer Res., 58, 1558
- Yasumoto S., 1996, Oncogene, 13, 433
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Items 101–107 of 107
Telomerase Control by Epigenetic Processes in Cellular Senescence
Chen, H., & Tollefsbol, T. O. (2009) · Springer eBooks · doi:10.1007/978-1-4419-0639-7_11 · Cited by 1
Lang, P. F., & Fröhlich, K. (2016) · doi:10.7287/peerj.preprints.1965v1 · Cited by 1
Telomerase reverse transcriptase (TERT) : promoter mutation and novel function in human cancer
Liu, T. (2014) · Figshare
TELOMERASE ACTIVATION AND ITS CLINICAL IMPLICATIONS IN HUMAN THYROID TUMORS
Wang, N. (2015) · Figshare
Bonifacio, L. N. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/nwfs-3n09
Etude de l'instabilité génomique et du statut des télomères dans le cancer du sein.
Gay‐Bellile, M. (2017) · theses.fr (ABES) · doi:10.70675/320a4136z144cz4b1ezb9e2z5cc2471afa58
Billard, P. (2021) · theses.fr (ABES) · doi:10.70675/7d145f6az2700z4c8bzad56z3286d493fb31
Metadata
- Authors
- Takakura, M., Kyo, S., Inoue, M., Wright, W. E., & Shay, J. W.
- Affiliations
- Kanazawa University (JP)
- The University of Texas Southwestern Medical Center (US)
- Corresponding author
- Shay, Jerry W.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2005-09-01
- Volume
- 25
- Issue
- 18
- Pages
- 8037-8043
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC1234330
- OpenAlex
- W2114840351
- Cited by
- 107
- References
- 47
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 6 · 2017: 6 · 2016: 5 · 2015: 6 · 2014: 7 · 2013: 7 · 2012: 7
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG07992, R01AG007992)
- National Cancer Institute (CA 70907, R01CA085363, P50CA070907, CA 85363)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- activator protein-1, telomerase reverse transcriptase, c-fos, c-Jun, JunD, AP-1 binding site, telomerase regulation, species-specific gene regulation, cancer cells, transcriptional repression
- MeSH terms
- Animals, Base Sequence, Binding Sites, DNA-Binding Proteins, HeLa Cells, Humans, Molecular Sequence Data, Mutation, Promoter Regions, Genetic, Transcription, Genetic, Transcriptional Activation, Gene Expression Regulation, Enzymologic, Proto-Oncogene Proteins c-jun, Proto-Oncogene Proteins c-fos, Transcription Factor AP-1, Telomerase, NIH 3T3 Cells, Mice
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