The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Genetic manipulation of centromere function.
Open accessAbstract
A conditional centromere was constructed in Saccharomyces cerevisiae by placing the centromere of chromosome III immediately downstream from the inducible GAL1 promoter from S. cerevisiae. By utilizing growth conditions that favor either transcriptional induction (galactose-carbon source) or repression (glucose-carbon source) from the GAL1 promoter, centromere function can be switched off or on, respectively. With the conditional centromere we were able to radically alter the mitotic transmission pattern of both monocentric and dicentric plasmids. Moreover, it was possible to selectively induce the loss of a single chromosome from a mitotically dividing population of cells. We observed that the induction of chromosome III aneuploidy resulted in a dramatic change in cell morphology. The construction of a conditional centromere represents a novel way to create conditional mutations of cis-acting DNA elements and will be useful for further analysis of this important stabilizing element.
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Citations
Cites
The Behavior in Successive Nuclear Divisions of a Chromosome Broken at Meiosis
McClintock, B. (1939) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.25.8.405 · Cited by 559
Cited by
The molecular basis for centromere identity and function
McKinley, K. L., & Cheeseman, I. M. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2015.5 · Cited by 668
Genome-Wide Mapping of the Cohesin Complex in the Yeast Saccharomyces cerevisiae
Glynn, E. F., Megee, P. C., Yu, H.-G., Mistrot, C., Unal, E., Koshland, D. E., DeRisi, J. L., & Gerton, J. L. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020259 · Cited by 431
Heat-Inducible Degron: a Method for Constructing Temperature-Sensitive Mutants
Dohmen, R. J., Wu, P., & Varshavsky, A. (1994) · Science · doi:10.1126/science.8122109 · Cited by 347
Nucleosome positioning can affect the function of a cis-acting DMA elementin vivo
Simpson, R. T. (1990) · Nature · doi:10.1038/343387a0 · Cited by 301
Molecular mechanisms of kinetochore capture by spindle microtubules
Tanaka, K., Mukae, N., Dewar, H., van Breugel, M., James, E. K., Prescott, A. R., Antony, C., & Tanaka, T. U. (2005) · Nature · doi:10.1038/nature03483 · Cited by 281
Metadata
- Authors
- Hill, A., & Bloom, K.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1987-07
- Volume
- 7
- Issue
- 7
- Pages
- 2397-2405
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1962277119
- Cited by
- 204
- References
- 1
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- green
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- Hill, A., & Bloom, K. (1987). Genetic manipulation of centromere function. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.7.7.2397
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
