The DOI matches its Crossref record; 75 of the 76 references it cites were found in a registry or an index.
Microbial NAD Metabolism: Lessons from Comparative Genomics
Open accessAbstract
SUMMARY: NAD is a coenzyme for redox reactions and a substrate of NAD-consuming enzymes, including ADP-ribose transferases, Sir2-related protein lysine deacetylases, and bacterial DNA ligases. Microorganisms that synthesize NAD from as few as one to as many as five of the six identified biosynthetic precursors have been identified. De novo NAD synthesis from aspartate or tryptophan is neither universal nor strictly aerobic. Salvage NAD synthesis from nicotinamide, nicotinic acid, nicotinamide riboside, and nicotinic acid riboside occurs via modules of different genes. Nicotinamide salvage genes nadV and pncA , found in distinct bacteria, appear to have spread throughout the tree of life via horizontal gene transfer. Biochemical, genetic, and genomic analyses have advanced to the point at which the precursors and pathways utilized by a microorganism can be predicted. Challenges remain in dissecting regulation of pathways.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“However, mammals do not have a homologous enzyme (12, 25–28).”
From Nicotinamide riboside and nicotinamide mononucleotide facilitate NAD + synthesis via enterohepatic circulation (2025) · cites this paper as [25]
The cited source says what the claim attributes to it.
In the citing paper
“Most bacteria have a NAM deamidase, an enzyme that converts NAM into NA.”
From Nicotinamide riboside and nicotinamide mononucleotide facilitate NAD + synthesis via enterohepatic circulation (2025) · cites this paper as [25]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
MrBayes 3: Bayesian phylogenetic inference under mixed models
Ronquist, F., & Huelsenbeck, J. P. (2003) · Bioinformatics · doi:10.1093/bioinformatics/btg180 · Cited by 29,194
Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd
Fleischmann, R. D., Adams, M. D., White, O. R., Clayton, R. A., Kirkness, E. F., Kerlavage, A. R., Bult, C. J., Tomb, J.-F., Dougherty, B. A., Merrick, J. M., et al. (1995) · Science · doi:10.1126/science.7542800 · Cited by 5,731
Environmental genome shotgun sequencing of the sargasso sea
Venter, J. C., Remington, K., Heidelberg, J. F., Halpern, A. L., Rusch, D., Eisen, J. A., Wu, D., Paulsen, I., Nelson, K. E., Nelson, W., et al. (2004) · Science · doi:10.1126/science.1093857 · Cited by 4,376
Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase
Imai, S.-I., Armstrong, C. M., Kaeberlein, M., & Guarente, L. (2000) · Nature · doi:10.1038/35001622 · Cited by 3,510
Bayesian Inference of Phylogeny and Its Impact on Evolutionary Biology
Huelsenbeck, J. P., Ronquist, F., Nielsen, R., & Bollback, J. P. (2001) · Science · doi:10.1126/science.1065889 · Cited by 2,737
Genes required for mycobacterial growth defined by high density mutagenesis
Sassetti, C. M., Boyd, D. H., & Rubin, E. J. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03425.x · Cited by 2,544
The Subsystems Approach to Genome Annotation and its Use in the Project to Annotate 1000 Genomes
Overbeek, R. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki866 · Cited by 2,019
Toward Automatic Reconstruction of a Highly Resolved Tree of Life
Ciccarelli, F. D., Doerks, T., von Mering, C., Creevey, C. J., Snel, B., & Bork, P. (2006) · Science · doi:10.1126/science.1123061 · Cited by 1,663
Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins
Frye, R. A. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.3000 · Cited by 1,409
Benson, D. A. (2004) · Nucleic Acids Research · doi:10.1093/nar/gki063 · Cited by 1,020
Cited by
Sorted by citations
Items 201–230 of 230
Borrelia burgdorferi: Carbon Metabolism and the Tick-Mammal Enzootic Cycle
Corona, A., & Schwartz, I. S. (2015) · ASM Press eBooks · doi:10.1128/9781555818883.ch8 · Cited by 1
Lee, H., Edgar, R. J., Lichtenstein, I. J., Velarde, J. J., Korotkova, N., & Wessels, M. R. (2022) · PLoS ONE · doi:10.1371/journal.pone.0270697 · Cited by 2
李超, 邹国珑, 张海潮, 林玉惠, & 赵丽青 (2023) · Food Nutrition Chemistry · doi:10.18686/zhfnc.v1i1.25 · Cited by 1
Novel NAD+ metabolomic technologies and their applications to Nicotinamide Riboside interventions
Trammell, S. A. J., Wright, M. E., Piper, R. C., Brenner, C., Slusarski, D. C., & Wilson, M. E. (2016) · doi:10.17077/etd.mk206led · Cited by 1
Self-citationCofactors and Coenzymes | Biochemistry: Niacin/NAD(P)
Bogan, K. L., & Brenner, C. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-819460-7.00515-6 · Cited by 1
Self-citationAshbrook, W. J. (2012) · Otago University Research Archive (University of Otago) · Cited by 1
Defining the mechanism of NAD-mediated mutagenesis
Ferguson, S. (2013) · Otago University Research Archive (University of Otago)
Thermococcus kodakarensis : the key to affordable biohydrogen production
Spaans, S. K. (2016) · doi:10.18174/377761
Sirtuins and life span extension
Lee, S., & Min, K. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-814118-2.00006-9
Telomere dysfunction impairs intestinal differentiation and predisposes to diet-induced colitisPreprint
Engevik, M. A., Guo, L., Song, W., Chronowski, C., Akhanov, V., Huerta, N. A. B., Engevik, A. C., Oezguen, N., Coarfa, C., Putluri, N., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.07.14.499988
Akanksha, A., & Mehra, S. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.05.19.541537
Microbial vitamins as nutraceuticals and their role as health-promoting agents
Ahmad, M. F., Ahmad, F. A., Ashraf, S. A., Alsayegh, A. A., Tabassum, F., Bantun, F., Elbandy, M., Shama, E., Uddin, S., & Khanam, A. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-15528-4.00010-6
Multifactorial microbial networks shape aging and neurodegeneration
Kara, N. Z., Savaliya, J. H., & Blacher, E. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-18979-1.00002-2
Yu, H., Shao, D., Zhao, Y., Lv, S., Tao, R., Sang, Y., Ren, J., Cooper, P. R., & Wang, Q. (2025) · The FASEB Journal · doi:10.1096/fj.202500944r
Sun, T., Yang, Y., Xie, J., Rao, Z., Han, R., & Cao, L. (2026) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2026.03.016
Repositioning of niacin in atherosclerotic cardiovascular disease
Li, W., Bai, Z., Li, C., Lu, S., Wang, Y., Huang, D., Zhang, H., Yuan, J., Zhen, X., & Liu, Y. (2026) · PharmaNutrition · doi:10.1016/j.phanu.2026.100506
Vitamins from Algae in Pharmaceuticals: Therapeutic Applications and Research Advances
De, J., & Dutta, M. (2026) · Springer eBooks · doi:10.1007/978-3-032-22521-4_15
Dubey, S., Sultana, K. N., Srivastava, A., & Srivastava, S. K. (2026) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2026.2676329
Schulz, A. M., Easterling, G. E., Balasubramaniyan, A., & Jewett, M. W. (2026) · Molecular Microbiology · doi:10.1111/mmi.70090
Phage-encoded NARP3 system rebuilds NAD + to subvert bacterial immunity
Jia, Y., Liu, S., Luo, Z., Zhi, S., Teng, G., Li, M., Ma, J., Gao, R., Qu, J., Huang, M., et al. (2026) · Science Advances · doi:10.1126/sciadv.aee9000
Hirayama, M., Takame, F., Maeda, T., Kashihara, K., Ito, M., Ohno, K., & Ueyama, J. (2026) · Gut Microbes · doi:10.1080/19490976.2026.2719062
A vitamin B 3 –driven root bacterial metabolite primes systemic immunity in Arabidopsis
Wang, X., Jiang, P., Xu, X., Zhang, J., Xia, G., Qian, W., Chen, Q., Wei, J., Liu, Z., Liu, L., et al. (2026) · Science Advances · doi:10.1126/sciadv.aef8959
A sequence motif enables widespread use of noncanonical redox cofactors in natural enzymes
Saleh, S., Hsu, N.-H., Luu, E., Martin, V. C., Ng, H. J. C., Black, W. B., Zhang, S., Kim, J., Sankaran, B., Tran, A. H. T., et al. (2026) · Nature Chemical Biology · doi:10.1038/s41589-026-02315-w · Cited by 2
Sun, W.-D., Bi, S.-Z., Zhu, X.-J., Lai, S.-Y., Zhang, C., Liu, A., & Li, J.-H. (2026) · Critical Reviews in Oncology/Hematology · doi:10.1016/j.critrevonc.2026.105622
Guo, X., Wang, Q., Liu, T., Hu, Y., Wang, X., & Zhao, Z. K. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2026.1954596
Mechanistic Insights into Nicotinamidase by CoII EPR Spectroscopy
Acosta, I. E., Bollmeyer, M. M., & Britt, R. D. (2026) · Inorganic Chemistry · doi:10.1021/acs.inorgchem.6c04023
Metadata
- Authors
- Gazzaniga, F., Stebbins, R., Chang, S. Z., McPeek, M. A., & Brenner, C.
- Affiliations
- Dartmouth College (US)
- University of Iowa (US)
- Venue
- Microbiology and Molecular Biology Reviews
- Publisher
- American Society for Microbiology
- Published
- 2009-09
- Volume
- 73
- Issue
- 3
- Pages
- 529-541
- ISSN
- 1092-2172 (ISSN-L)
- 1098-5557
- PMC
- PMC2738131
- OpenAlex
- W2055192452
- Cited by
- 230
- References
- 76
- Citations by year
- 2026: 17 · 2025: 17 · 2024: 19 · 2023: 18 · 2022: 25 · 2021: 14 · 2020: 21 · 2019: 15 · 2018: 16 · 2017: 7 · 2016: 15 · 2015: 5 · 2014: 13 · 2013: 5 · 2012: 6
- FWCI
- 0.59
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Microbiology and Molecular Biology Reviews (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Dartmouth College
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- NAD+ metabolism, NAD biosynthesis, nicotinamide riboside, horizontal gene transfer, pncA, comparative genomics
- MeSH terms
- Bacteria, NAD, Genome, Bacterial, Sequence Homology, Amino Acid, Evolution, Molecular, Gene Transfer, Horizontal, Genomics, Biosynthetic Pathways
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
