The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Biosynthesis of Polyketides in Heterologous Hosts
Open accessAbstract
SUMMARY: Polyketide natural products show great promise as medicinal agents. Typically the products of microbial secondary biosynthesis, polyketides are synthesized by an evolutionarily related but architecturally diverse family of multifunctional enzymes called polyketide synthases. A principal limitation for fundamental biochemical studies of these modular megasynthases, as well as for their applications in biotechnology, is the challenge associated with manipulating the natural microorganism that produces a polyketide of interest. To ameliorate this limitation, over the past decade several genetically amenable microbes have been developed as heterologous hosts for polyketide biosynthesis. Here we review the state of the art as well as the difficulties associated with heterologous polyketide production. In particular, we focus on two model hosts, Streptomyces coelicolor and Escherichia coli. Future directions for this relatively new but growing technological opportunity are also discussed.
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Developing a new resource for drug discovery: marine actinomycete bacteria
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Type II polyketide synthases: gaining a deeper insight into enzymatic teamwork
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Metadata
- Authors
- Pfeifer, B. A., & Khosla, C.
- Venue
- Microbiology and Molecular Biology Reviews
- Publisher
- American Society for Microbiology
- Published
- 2001-03
- Volume
- 65
- Issue
- 1
- Pages
- 106-118
- ISSN
- 1092-2172 (ISSN-L)
- 1098-5557
- OpenAlex
- W2132042949
- Cited by
- 262
- References
- 65
- FWCI
- 1.28
- Type
- journal article
- Language
- en
- Open access
- green
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