Amine neuromediators, their precursors, and oxidation products in the culture of Escherichia coli k-12
Abstract
Growth dynamics of the synthesis of monoamine neuromediators serotonin, norepinephrine, and dopamine in Escherichia coli K-12 was investigated for the first time using high performance liquid chromatography with electrodetection. Maximum (micromolar) concentrations of these compounds were detected in E. coli cells during the early growth phases; their intracellular content decreases after the transition to late growth phases. E. coli biomass contains the substances DOPA and 5-hydroxytryptamine that serve in animal cells as neuromediator precursors and the products of their oxidative deamination. Presumably, the biosynthesis and degradation of monoamine neuromediators in bacterial cells involves enzyme systems analogous to those typical of animals. The culture fluid of E. coli contains micromolar concentrations of DOPA and nanomolar of serotonin, dopamine, and norepinephrine during the late growth phase. These concentrations are sufficient for animal/human receptors to bind them. This article deals with the potential biotechnological applications of the data obtained.
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Some microbes residing in the gut can produce serotonin.
“and some of the microbes residing in the gut can produce serotonin (i.e., Lactococcus lactis subsp.cremoris, L. lactis subsp.Lactis, Lactobacillus plantarum, Streptococcus thermophilus, E. coli K-12, Morganella morganii, Klebsiella pneumonia) [110,112,113].”
From Antimicrobial Properties of Antidepressants and Antipsychotics—Possibilities and Implications (2021) · cites this paper as [113]
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- Authors
- Shishov, V. A., Kirovskaya, T. A., Kudrin, V. S., & Oleskin, A. V.
- Affiliations
- Lomonosov Moscow State University (RU)
- Corresponding author
- Oleskin, A. V.
- ORCID
- Oleskin, A. V.
- Venue
- Applied Biochemistry and Microbiology
- Publisher
- Pleiades Publishing Ltd
- Published
- 2009-09
- Volume
- 45
- Issue
- 5
- Pages
- 494-497
- ISSN
- 0003-6838 (ISSN-L)
- 1608-3024
- OpenAlex
- W2042220721
- Cited by
- 174
- References
- 2
- Citations by year
- 2026: 4 · 2025: 16 · 2024: 23 · 2023: 14 · 2022: 20 · 2021: 26 · 2020: 17 · 2019: 14 · 2018: 13 · 2017: 11 · 2016: 8 · 2014: 6
- FWCI
- 0.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Applied Biochemistry and Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- monoamines, serotonin, norepinephrine, dopamine, DOPA, Escherichia coli K-12, bacterial growth phase, neurotransmitter synthesis, neurotransmitter precursors, oxidative deamination, biotechnology applications
- MeSH terms
- Chromatography, High Pressure Liquid, Diterpenes, Dihydroxyphenylalanine, Dopamine, Norepinephrine, Oxidation-Reduction, Serotonin, Neurotransmitter Agents, Escherichia coli K12
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