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Cited by 310

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport

Open access
Ménard, S, Candalh, C, Bambou, J C, Terpend, K, Cerf-Bensussan, N, Heyman, M·2004·Gut
DOI 10.1136/gut.2003.026252
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Abstract

Background: Probiotic bacteria have a beneficial effect on intestinal inflammation. In this study, we have examined the effect of lactic acid and commensal Gram positive (+) bacteria conditioned media (CM) on tumour necrosis factor α (TNF-α) release and the mechanisms involved. Methods: Lipopolysaccharide (LPS) induced TNF-α secretion by peripheral blood mononuclear cells or the THP-1 cell line was monitored in the presence or absence of bacteria CM obtained from two probiotic strains, Bifidobacterium breve ( Bb ) and Streptococcus thermophilus ( St ), and three commensal bacterial strains ( Bifidobacterium bifidum , Ruminococcus gnavus , and unidentified Streptococcus ). Bb and St bacteria CM were allowed to cross filter grown intestinal epithelial cell monolayers (HT29-19A) to assess intestinal transport of active bacterial products. These products were characterised and their effect on LPS binding to THP-1 cells and nuclear factor κB (NFκB) activation assessed. Results: Dose dependent inhibition of LPS induced TNF-α secretion was noted for both probiotic bacteria CM (64% and 71% inhibition for Bb and St , respectively) and to a lesser extent commensal bacteria CM (21–32% inhibition). Active products from Bb and St were resistant to digestive enzymes and had a molecular mass <3000 Da. Their inhibitory effect was preserved after transepithelial transport across intestinal cell monolayers, mainly in inflammatory conditions. LPS-FITC binding to THP-1 cells and NFκB activation were significantly inhibited by Bb and St CM. Conclusion: B breve and S thermophilus release metabolites exerting an anti-TNF-α effect capable of crossing the intestinal barrier. Commensal bacteria also display a TNF-α inhibitory capacity but to a lesser extent. These results underline the beneficial effect of commensal bacteria in intestinal homeostasis and may explain the role of some probiotic bacteria in alleviating digestive inflammation.

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Metadata

Authors
Ménard, S., Candalh, C., Bambou, J. C., Terpend, K., Cerf-Bensussan, N., & Heyman, M.
Venue
Gut
Publisher
BMJ
Published
2004-05-11
Volume
53
Issue
6
Pages
821-828
ISSN
  • 0017-5749 (ISSN-L)
  • 1468-3288
DOI
10.1136/gut.2003.026252
OpenAlex
W2056010853
Cited by
310
References
1
FWCI
24.07
Type
journal article
Language
en
Open access
bronze

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