Cited by 590Probiotics and Fermented FoodsGastrointestinal motility and disordersGut microbiota and health

Secreted bioactive factors from Bifidobacterium infantis enhance epithelial cell barrier function

Ewaschuk, Julia B., Diaz, Hugo, Meddings, Liisa, Diederichs, Brendan, Dmytrash, Andrea, Backer, Jody, Looijer-van Langen, Mirjam, Madsen, Karen L.·2008·American Journal of Physiology-Gastrointestinal and Liver Physiology

Abstract

Live probiotic bacteria are effective in reducing gut permeability and inflammation. We have previously shown that probiotics release peptide bioactive factors that modulate epithelial resistance in vitro. The objectives of this study were to determine the impact of factors released from Bifidobacteria infantis on intestinal epithelial cell permeability and tight junction proteins and to assess whether these factors retain their bioactivity when administered to IL-10-deficient mice. B. infantis conditioned medium (BiCM) was applied to T84 human epithelial cells in the presence and absence of TNF-alpha and IFN-gamma. Transepithelial resistance (TER), tight junction proteins [claudins 1, 2, 3, and 4, zonula occludens (ZO)-1, and occludin] and MAP kinase activity (p38 and ERK) were examined. Acute effects of BiCM on intestinal permeability were assessed in colons from IL-10-deficient mice in Ussing chambers. A separate group of IL-1-deficient mice was treated with BiCM for 4 wk and then assessed for intestinal histological injury, cytokine levels, epithelial permeability, and immune response to bacterial antigens. In T84 cells, BiCM increased TER, decreased claudin-2, and increased ZO-1 and occludin expression. This was associated with enhanced levels of phospho-ERK and decreased levels of phospho-p38. BiCM prevented TNF-alpha- and IFN-gamma-induced drops in TER and rearrangement of tight junction proteins. Inhibition of ERK prevented the BiCM-induced increase in TER and attenuated the protection from TNF-alpha and IFN-gamma. Oral BiCM administration acutely reduced colonic permeability in mice whereas long-term BiCM treatment in IL-10-deficient mice attenuated inflammation, normalized colonic permeability, and decreased colonic and splenic IFN-gamma secretion. In conclusion, peptide bioactive factors from B. infantis retain their biological activity in vivo and are effective in normalizing gut permeability and improving disease in an animal model of colitis. The effects of BiCM are mediated in part by changes in MAP kinases and tight junction proteins.

Integrity signals

Registry

Crossref · 10.1152/ajpgi.90227.2008

Cites

50 of 50 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Ewaschuk, J. B., Diaz, H., Meddings, L., Diederichs, B., Dmytrash, A., Backer, J., Looijer-van Langen, M., & Madsen, K. L.
Affiliations
University of Alberta (CA)
Venue
American Journal of Physiology-Gastrointestinal and Liver Physiology
Published
2008-11
Volume
295
Issue
5
Pages
G1025-G1034
ISSN
  • 0193-1857 (ISSN-L)
  • 1522-1547
OpenAlex
W2141878383
Cited by
590
References
50
Citations by year
2026: 18 · 2025: 24 · 2024: 24 · 2023: 51 · 2022: 34 · 2021: 45 · 2020: 34 · 2019: 25 · 2018: 37 · 2017: 43 · 2016: 42 · 2015: 39 · 2014: 33 · 2013: 37 · 2012: 27
FWCI
41.14
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Bifidobacterium infantis, intestinal epithelial barrier function, intestinal permeability, tight junction proteins, claudin-2, ZO-1, occludin, MAPK signaling, ERK, p38 MAPK, TNF-α, interferon-gamma, IL-10-deficient mice, colitis, colonic inflammation, oral administration
MeSH terms
Animals, Bifidobacterium, Gene Expression Regulation, Humans, Intestines, Membrane Proteins, Phosphorylation, Interleukin-10, Culture Media, Conditioned, Gene Deletion, Probiotics, Mitogen-Activated Protein Kinase Kinases, Cell Line, Tumor, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review