First phenotypic description of transferrin receptor 2 knockout mouse, and the role of hepcidin
Open accessAbstract
Background: Transferrin receptor 2 (TfR2) is a key molecule involved in the regulation of iron homeostasis. Mutations in humans cause type 3 haemochromatosis and a targeted mutation in mice leads to iron overload with a similar phenotype. We have previously described the generation of a complete TfR2 -knockout (KO) mouse. Aims: The aims of this study were to determine the phenotype and analyse expression of iron related molecules in the liver, duodenum, and spleen of homozygous TfR2 -KO, heterozygous, and wild-type mice. Methods: Serum and tissue iron levels were determined in 10 week old male mice. Expression of iron related mRNA transcripts were analysed in the liver, duodenum, and spleen using real time polymerase chain reaction. Expression of iron related proteins in the liver were analysed by immunoblotting and immunohistochemistry. Results: Homozygous TfR2 -KO mice had no TfR2 protein expression and developed significant iron overload typical of TfR2 associated haemochromatosis. In the liver of TfR2 -KO mice there was no upregulation of hepcidin mRNA or prohepcidin protein in response to iron loading. Conclusions: Our results suggest that TfR2 is required for iron regulated expression of hepcidin and is involved in a pathway related to Hfe and hemojuvelin.
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Serum ferritin is identical to cytoplasmic ferritin, but the primary component of serum ferritin is the ferritin L subunit, which contains little iron.
“Serum ferritin is identical to cytoplasmic ferritin, but the primary component of serum ferritin is the ferritin L subunit, which contains little iron (323).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [323]
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Self-citation
- Authors
- Wallace, D. F., Summerville, L., Lusby, P. E., & Subramaniam, V. N.
- Affiliations
- QIMR Berghofer Medical Research Institute (AU)
- Corresponding author
- Subramaniam, V N
- Venue
- Gut
- Publisher
- BMJ
- Published
- 2005-06-10
- Volume
- 54
- Issue
- 7
- Pages
- 980-986
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1774629
- OpenAlex
- W2003255834
- Cited by
- 160
- References
- 34
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 7 · 2020: 4 · 2019: 2 · 2018: 6 · 2017: 3 · 2016: 6 · 2015: 8 · 2014: 7 · 2013: 7 · 2012: 7
- FWCI
- 8.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Institutes of Health (1r01dk057648-01)
- Medical Research Council
- National Health and Medical Research Council
- National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK057648, 5R01-DK057648-04)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Keywords
- transferrin receptor 2, hepcidin, iron homeostasis, iron overload, HFE gene, hemojuvelin, liver iron, duodenum, spleen, iron regulatory genes
- MeSH terms
- Animals, Duodenum, Gene Expression Regulation, Immunoenzyme Techniques, Liver, Male, Mice, Inbred C57BL, Phenotype, Receptors, Transferrin, RNA, Messenger, Spleen, Polymerase Chain Reaction, Mice, Knockout, Iron Overload, Antimicrobial Cationic Peptides, Mice, Hepcidins
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