Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 34,401–34,500 of 48,846

A Novel Lysophosphatidic Acid Acyltransferase of Escherichia coli Produces Membrane Phospholipids with a cis-vaccenoyl Group and Is Related to Flagellar Formation
Toyotake, Y., Nishiyama, M., Yokoyama, F., et al. (2020) · Biomolecules · doi:10.3390/biom10050745 · Cited by 10
“The frozen cells were lyophilized, and total PLs were extracted using the Bligh and Dyer method [42].”
Method
Screening Suitability of Northern Hemisphere Algal Strains for Heterotrophic Cultivation and Fatty Acid Methyl Ester Production
Nzayisenga, J. C., Niemi, C., Ferro, L., Gorzsás, A., Gentili, F. G., Funk, C., & Sellstedt, A. (2020) · Molecules · doi:10.3390/molecules25092107 · Cited by 11
Changes in biochemical composition of cassava and beet residues during solid state bioprocess with Pleurotus ostreatus
Araújo, S. D. S., Silva, P. G. P., Araújo, S. D. S., et al. (2020) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2020.101641 · Cited by 10
Adipose tissue-liver crosstalk during pathologic changes caused by vinyl chloride metabolites in mice
Kaelin, B. R., McKenzie, C. M., Hempel, K. W., Lang, A. L., Arteel, G. E., & Beier, J. I. (2020) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2020.115068 · Cited by 10
“Lipids were extracted from snap-frozen samples as described previously (Bligh and Dyer, 1959; Kaiser et al., 2009).”
Method
Evaluation of two lipid removal methods for stable carbon and nitrogen isotope analysis in whale tissue
Smith, K. J., Trueman, C. N., France, C. A. M., et al. (2020) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.8851 · Cited by 10
Rearing enhancement of Ovalipes trimaculatus (Crustacea: Portunidae) zoea I by feeding on Artemia persimilis nauplii enriched with alternative microalgal diets
Martelli, A., Barbieri, E., Dima, J. B., et al. (2020) · Scientific Reports · doi:10.1038/s41598-020-67933-3 · Cited by 10
Extracts of the green algae Ulva prolifera possess antioxidant and antibacterial activities in vitro
Hafez, M. S. M. A. E., Elkomy, R. G., Saleh, H., et al. (2020) · Egyptian Journal of Aquatic Biology and Fisheries · doi:10.21608/ejabf.2020.98005 · Cited by 10
“The extraction of lipids was done according to a chloroform-methanol method (Bligh and Dyer, 1959), and the results were expressed in percentage.”
Method
Algal Biomass for Biofuels and Bioproducts
Srinivasan, B., & Kulshreshtha, G. (2020) · The handbook of environmental chemistry · doi:10.1007/698_2020_580 · Cited by 12
Hepatic Clearance Binding Terms of Hydrophobic Organic Chemicals in Rainbow Trout: Application of a Streamlined Sorbent-Phase Dosing Method
Saunders, L. J., Diaz-Blanco, G., Lee, Y., et al. (2020) · Environmental Science & Technology Letters · doi:10.1021/acs.estlett.0c00518 · Cited by 10
Foraging behavior of yellow-phase Japanese eels between connected fresh- and brackish water habitats
Kutzer, A., Lavergne, É., Kume, M., et al. (2020) · Environmental Biology of Fishes · doi:10.1007/s10641-020-01002-6 · Cited by 10
The role of mesenteric lymph exosomal lipid mediators following intestinal ischemia-reperfusion injury on activation of inflammation
Senda, A., Morishita, K., Kojima, M., Doki, S., Taylor, B. A., Yagi, M., Watanabe, A., Kobayashi, T., Aiboshi, J., Coimbra, R. S. M., et al. (2020) · The Journal of Trauma: Injury, Infection, and Critical Care · doi:10.1097/ta.0000000000002897 · Cited by 10
Effects of freeze-dried pulp of Eugenia uniflora L. and Opuntia ficus-indica fruits on quality attributes of beef patties enriched with n-3 fatty acids
Romero, M. C., Fogar, R. A., Fernández, C. L., et al. (2020) · Journal of Food Science and Technology · doi:10.1007/s13197-020-04703-z · Cited by 10
Soymilk fermentation: effect of cooling protocol on cell viability during storage and in vitro gastrointestinal stress
Piazentin, A. C. M., da Silva, T. M. S., Florence-Franco, A. C., et al. (2020) · Brazilian Journal of Microbiology · doi:10.1007/s42770-020-00369-z · Cited by 10
Increased Antioxidant Capacity and Pro-Homeostatic Lipid Mediators in Ocular Hypertension—A Human Experimental Model
Langbøl, M. A., Saruhanian, S., Baskaran, T., Tiedemann, D., Mouhammad, Z. A., Toft‐Kehler, A. K., Jun, B., Vohra, R., Bazan, N. G., & Kolko, M. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9092979 · Cited by 11
“A modified Bligh–Dyer method [34] was used for lipid extraction.”
Method
Prevention of the rise in plasma cholesterol and glucose levels by kaki‐tannin and characterization of its bile acid binding capacity
Nishida, S., Katsumi, N., & Matsumoto, K. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10834 · Cited by 10
Investigating metabolism by mass spectrometry: From steady state to dynamic view
Audano, M., Pedretti, S., Ligorio, S., Giavarini, F., Caruso, D., & Mitro, N. (2020) · Journal of Mass Spectrometry · doi:10.1002/jms.4658 · Cited by 11
Consequences of oxidative damage on the fatty acid profile in muscle of Cichlasoma amazonarum acutely exposed to copper
Baldissera, M. D., Souza, C. F., Barroso, D. C., Pereira, R. S., de Oliveira, F. D. C., Alessio, K. O., Wagner, R. C., Bizzi, C. A., Baldisserotto, B., & Val, A. L. (2020) · Fish Physiology and Biochemistry · doi:10.1007/s10695-020-00884-8 · Cited by 9
Phycoremediation of X-ray developer solution towards silver removal with concomitant lipid production
Sharma, S., & Garlapati, V. K. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.115837 · Cited by 10
Physaria fendleri and Ricinus communis lecithin:cholesterol acyltransferase‐like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine
Xu, Y., Caldo, K. M. P., Singer, S. D., Mietkiewska, E., Greer, M. S., Tian, B., Dyer, J. M., Smith, M. A., Zhou, X., Qiu, X. K., et al. (2020) · The Plant Journal · doi:10.1111/tpj.15050 · Cited by 10
Dietary Alaska pollock protein attenuates liver steatosis and alters gut microbiota in leptin-deficient ob/ob mice
Maeda, H., Hosomi, R., Yokoyama, T., Ikeda, Y., Nishimoto, A., Tanaka, G., Shimono, T., Kanda, S., Nishiyama, T., Yoshida, M., et al. (2020) · Journal of Functional Foods · doi:10.1016/j.jff.2020.104266 · Cited by 10
Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes
Supruniuk, E., Mikłosz, A., & Chabowski, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21218382 · Cited by 11
“Lipid fractions were extracted from L6 cells using a Bligh and Dyer method [89], i.”
Method
Beneficial Effects of Spirulina Aqueous Extract on Vasodilator Function of Arteries from Hypertensive Rats
Villalpando, D. M., Verdasco‐Martín, C. M., Plaza, I., Rivas, J. G., de Bethencourt, F. R., Villarroel, M., García, J. L., Otero, C., & Ferrer, M. (2020) · International Journal of Vascular Medicine · doi:10.1155/2020/6657077 · Cited by 11
“A polar Spirulina extract was prepared by solvent extraction according to the Bligh and Dyer method [27] after a prior step of Spirulina biomass sonication.”
Method
Separation of regioisomers and enantiomers of triacylglycerols containing branched fatty acids (iso and/or anteiso)
Palyzová, A., Cajthaml, T., & Řezanka, T. (2020) · Electrophoresis · doi:10.1002/elps.202000320 · Cited by 10
Halomonas icarae sp. nov., a moderately halophilic bacterium isolated from beach soil in India
Pandiyan, K., Kushwaha, P., Bagul, S. Y., et al. (2020) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.004611 · Cited by 10
Manipulation of Chlorella vulgaris polyunsaturated ω‐3 fatty acid profile by supplementation with vegetable amino acids and fatty acids
Siahbalaei, R., Kavoosi, G., & Noroozi, M. (2020) · Phycological Research · doi:10.1111/pre.12449 · Cited by 10
“In comparison with the conventional method by using chloroform and methanol, one-step in situ extraction with hexane is more applicable for the rapid quantitative assessment of fatty acids and more effective in reducing environmental pollution (Bligh and Dyer 1959).”
Method
Sphingolipidomics in Translational Sepsis Research–Biomedical Considerations and Perspectives
Claus, R. A., & Gräler, M. H. (2021) · Frontiers in Medicine · doi:10.3389/fmed.2020.616578 · Cited by 11
Effects of dietary fish oil replacement by poppy seed oil on growth performance and fillet quality of rainbow trout (Oncorhynchus mykiss)
Örnek, E., Acar, Ü., & ÖĞÜTCÜ, M. (2021) · Aquaculture Research · doi:10.1111/are.15147 · Cited by 10
Calculation of full process freezing time in minced fish muscle
Cuesta, F. J., Sánchez‐Alonso, I., Navas, A., et al. (2021) · MethodsX · doi:10.1016/j.mex.2021.101292 · Cited by 10
Composition of uterine milk and its changes with gestational period in red stingrays (Hemitrygon akajei)
Kina, T., Masuda, T., Asahina, K., & Suzuki, M. (2021) · Journal of Fish Biology · doi:10.1111/jfb.14716 · Cited by 10
Efficient use of biomass and extract of the microalga Desmodesmus subspicatus (Scenedesmaceae) in asymbiotic seed germination and seedling development of the orchid Cattleya warneri
Navarro, Q. R., Corrêa, D. D. O., Behling, A., Noseda, M. D., Amano, É., Suzuki, R. M., & Ribas, L. L. F. (2021) · Journal of Applied Phycology · doi:10.1007/s10811-021-02442-y · Cited by 11
Plasma epoxyeicosatrienoic acids and dihydroxyeicosatrieonic acids, insulin, glucose and risk of diabetes: The strong heart study
Lemaître, R. N., Jensen, P. N., Zeigler, M. B., Denham, J., Fretts, A. M., Umans, J. G., Howard, B. V., Sitlani, C. M., McKnight, B., Gharib, S. A., et al. (2021) · EBioMedicine · doi:10.1016/j.ebiom.2021.103279 · Cited by 10
“Briefly, total lipids were extracted using a modified Bligh and Dyer method; [31] phospholipids were hydrolyzed by saponification to release fatty acids; the fatty acids were extracted by solid phase extraction and derivatized by a modified Bollinger method; [32] eicosanoids were then separated and quantitated by ultr…”
Method
Promiscuous phospholipid biosynthesis enzymes in the plant pathogen Pseudomonas syringae
Vasilopoulos, G., Moser, R., Petersen, J., Aktas, M., & Narberhaus, F. (2021) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2021.158926 · Cited by 10
Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse
Paget, T. L., Parkinson-Lawrence, E. J., Trim, P. J., Autilio, C., Panchal, M. H., Koster, G., Echaide, M., Snel, M. F., Postle, A. D., Morrison, J. L., et al. (2021) · Cells · doi:10.3390/cells10040849 · Cited by 10
“Lavaged lung tissue, with 10 µ L of the antioxidant butylated hydroxyl toludene (BHT) added, was homogenised in saline using a Heidolph Silent Crusher S prior to storing a − 80 ◦ C. Phospholipids were extracted from both lung tissue homogenates and BALF using the method of Bligh and Dyer [23].”
Interaction of amphiphilic lipoarabinomannan with host carrier lipoproteins in tuberculosis patients: Implications for blood-based diagnostics
Jakhar, S., Sakamuri, R. M., Vu, D. M., et al. (2021) · PLoS ONE · doi:10.1371/journal.pone.0243337 · Cited by 10
“For this assay, LAM is released from host lipoprotein complexes prior to detection via a pre-established sample processing method [28].”
Method
Heterotrophic and Mixotrophic Cultivation of Chlorella vulgaris using Chicken Waste Compost as Nutrients Source for Lipid Production
Tan, H. L., Lam, M. K., Cheng, Y. W., Lim, J. W., Tan, I. S., Foo, C. Y. H., & Show, P. L. (2021) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/721/1/012011 · Cited by 11
“Bligh and Dyer (1959) method with modification [16].”
Method
Targeted Lipidomics of Drosophila melanogaster During Development
Goh, E. X. Y., & Guan, X. L. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1410-5_13 · Cited by 9
The content of carotenoids and tocochromanols in bitter, semi-sweet and sweet apricots depending on different harvest times and geographical regions
Cherif, A. O., Messaouda, M. B., Abderrabba, M., et al. (2021) · European Food Research and Technology · doi:10.1007/s00217-021-03688-z · Cited by 10
In situ monitoring of chlorophyll a fluorescence in Nannochloropsis oceanica cultures to assess photochemical changes and the onset of lipid accumulation during nitrogen deprivation
Carneiro, M., Chini Zittelli, G., Cicchi, B., Touloupakis, E., Faraloni, C., Maia, I. B., Pereira, H., Santos, T., Malcata, F. X., Otero, A., et al. (2021) · Biotechnology and Bioengineering · doi:10.1002/bit.27906 · Cited by 10
Isolation of Lipid Droplets for Protein and Lipid Analysis
Horn, P. J., Chapman, K. D., & Ischebeck, T. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1362-7_16 · Cited by 10
Effect of Technological Process on True Retention Rate of Eicosapentaenoic and Docosahexaenoic Acids, Lipid Oxidation and Physical Properties of Canned Smoked Sprat (Sprattus sprattus)
Domiszewski, Z., & Mierzejewska, S. (2021) · International Journal of Food Science · doi:10.1155/2021/5539376 · Cited by 10
“Lipids from raw, smoked fish, and solid and liquid parts of canned fish products were extracted with chloroform and methanol, using the Bligh-Dyer method [12].”
Method
Carbonization of microalgae for bio-coal production as a solid biofuel similar to bituminous coal
Guajardo, D., Valdebenito, F., Díaz, J., Cifuentes, G., & Azócar, L. (2021) · Fuel · doi:10.1016/j.fuel.2021.120963 · Cited by 9
Autochthonous production contributes to the diet of wood-boring invertebrates in temperate shallow water
Nishimoto, A., Haga, T., Asakura, A., et al. (2021) · Oecologia · doi:10.1007/s00442-021-04973-0 · Cited by 10
Milk from cows fed clover-rich silage compared with cows fed grass silage is higher in n-3 fatty acids
Lashkari, S., Johansen, M., Weisbjerg, M. R., & Jensen, S. K. (2021) · Journal of Dairy Science · doi:10.3168/jds.2021-20407 · Cited by 10
Enhanced lipid accumulation of Chlorella vulgaris with agricultural waste under optimized photoheterotrophic conditions
Tekin, N., Ergörünlü, B., Karatay, S. E., & Dönmez, G. (2021) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-021-01793-3 · Cited by 10
Streptomonospora litoralis sp. nov., a halophilic thiopeptides producer isolated from sand collected at Cuxhaven beach
Khodamoradi, S., Hahnke, R. L., Mast, Y., et al. (2021) · Antonie van Leeuwenhoek · doi:10.1007/s10482-021-01609-4 · Cited by 10
Effect of dietary milk thistle (Silybum marianum L.) oil supplementation on animal performance and milk fatty acid composition in dairy ewes
Karaiskou, C., Kasapidou, E., Michailidis, G., Markantonatos, X., & Basdagianni, Z. (2021) · Small Ruminant Research · doi:10.1016/j.smallrumres.2021.106493 · Cited by 10
Pathogenic Variants in ABHD16A Cause a Novel Psychomotor Developmental Disorder With Spastic Paraplegia
Yahia, A., Elsayed, L. E. O., Valter, R., et al. (2021) · Frontiers in Neurology · doi:10.3389/fneur.2021.720201 · Cited by 10
“Extraction of Phospholipids We extracted lipids from cells using the Bligh & Dyer method (18).”
Method
Effects of dietary inclusion of yerba mate ( Ilex paraguariensis ) extract on lamb muscle metabolomics and physicochemical properties in meat
Lobo, R. R., Correia, B. S. B., Pena-Bermudez, Y. A., Vincenzi, R., da Silva, C. M., Panosso, L. L., Ceribeli, C., Colnago, L. A., Cardoso, D. R., Berndt, A., et al. (2021) · Journal of Animal Science · doi:10.1093/jas/skab244 · Cited by 10
HILIC‐ESI‐MS analysis of phosphatidic acid methyl esters artificially generated during lipid extraction from microgreen crops
Castellaneta, A., Losito, I., Losacco, V., et al. (2021) · Journal of Mass Spectrometry · doi:10.1002/jms.4784 · Cited by 10
“Specifically, lipids were extracted from microgreens of chia, soy, flax, sunflower and rapeseed, using a modified version of the Bligh and Dyer protocol.(21) The procedure was applied to previously lyophilized microgreens after homogenization into water.”
Method
Algibacter onchidii sp. nov., a symbiotic bacterium isolated from a marine invertebrate
Yin, Q., Liang, J., Zheng, X., et al. (2021) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.005102 · Cited by 10
Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in Rhodosporidium toruloides
Deeba, F., Kumar, K. K., Rajacharya, G. H., et al. (2021) · Journal of Fungi · doi:10.3390/jof7110967 · Cited by 10
“Modified Bligh and Dyer procedure was used for total lipid extraction and were measured gravimetrically after drying under N2 [13].”
Method
A microalgal biorefinery approach for bioactive molecules, biofuel, and biofertilizer using a novel carbon dioxide-tolerant strain Tetradesmus obliquus CT02
Sinha, A., Goswami, G., Kumar, R., & Das, D. (2021) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-021-02098-1 · Cited by 10
“The intracellular lipid was estimated by thermo-gravimetric analysis [14].”
Method
Fatty Acid Composition and Volatile Profile of longissimus thoracis et lumborum Muscle from Burguete and Jaca Navarra Foals Fattened with Different Finishing Diets
Cittadini, A., Domínguez, R., Pateiro, M., et al. (2021) · Foods · doi:10.3390/foods10122914 · Cited by 10
“For fatty acid determination, total fat was extracted according to the method described by Bligh and Dyer [39], with modifications.”
Method
The Sphingolipid Asset Is Altered in the Nigrostriatal System of Mice Models of Parkinson’s Disease
Blokhin, V. E., Shupik, M. A., Gutner, U., Pavlova, E., Lebedev, А. T., Maloshitskaya, O. A., Bogdanov, V. V., Sokolov, S. A., Alessenko, A. V., & Ugrumov, M. V. (2022) · Biomolecules · doi:10.3390/biom12010093 · Cited by 10
“Lipids were extracted from the striatum and the SN according to the Bligh and Dyer method [26].”
Method
Potential Ophthalmological Application of Extracts Obtained from Tuna Vitreous Humor Using Lactic Acid-Based Deep Eutectic Systems
Abdallah, M. M., Leonardo, I. C., Krstić, L., Enríquez‐de‐Salamanca, A., Diebold, Y., González-García, M. J., Gaspar, F. B., Matias, A. A., Bronze, M. R., & Fernández, N. (2022) · Foods · doi:10.3390/foods11030342 · Cited by 10
“References [61,62] are cited in the supplementary materials.”
Background
Clinical and Microbiological Performances and Effects on Lipid and Cytokine Production of a Ceruminolytic Ear Cleaner in Canine Erythemato-Ceruminous Otitis Externa
Moog, F., Mivielle, J., Brun, J., et al. (2022) · Veterinary Sciences · doi:10.3390/vetsci9040185 · Cited by 10
“Neutral lipids (free and bound) were analysed by gas chromatography combined with mass spectrometry (GC/MS) after Bligh and Dyer liquid/liquid extraction [24].”
Method
Synthesis and Investigation of Novel CHCA-Derived Matrices for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Lipids
Monopoli, A., Ventura, G., Aloia, A., et al. (2022) · Molecules · doi:10.3390/molecules27082565 · Cited by 10
Development and Characterization of Powdered Antioxidant Compounds Made from Shiraz (Vitis vinifera L.) Grape Peels and Arrowroot (Maranta arundinacea L.)
Souza, E. L., Nascimento, T. S., Magalhães, C. M., Barreto, G. D. A., Leal, I. L., dos Anjos, J. P., & Machado, B. A. S. (2022) · The Scientific World JOURNAL · doi:10.1155/2022/7664321 · Cited by 12
“Total lipids were extracted and quantified according to Bligh and Dyer’s methodology [44].”
Method
Impacts of Nano-Gelatin Coating Containing Thymol and Nisin on Chemical Quality Indices of Rainbow Trout Fillets Stored at 4°C
Hashemi, M. B., Pourmousavi, F. S., Mohajer, F., et al. (2022) · Jundishapur Journal of Natural Pharmaceutical Products · doi:10.5812/jjnpp-122177 · Cited by 10
“Lipid extraction from fish fillet samples was carried out as per the method of Bligh and Dyer (13) with minor modifications conducive to reducing the use of methanol and chloroform.”
Method
Discrimination of South American grains based on fatty acid
Ferreira, D. S., Rocha, J. C. B., Arellano, D. B., & Pallone, J. A. L. (2022) · Quality Assurance and Safety of Crops & Foods · doi:10.15586/qas.v14i3.1064 · Cited by 10
“Table 1 presents the total lipid content analyzed by Bligh and Dyer (1959), and the range of lipid content for each grain, its origin, mean, and standard deviation for each sample, with its code.”
Background
Effect of Fungal Fermentation on Enhancement of Nutritional Value and Antioxidant Activity of Defatted Oilseed Meals
Dutta, R., Ghosal, S., Bhattacharyya, D. K., & Bhowal, J. (2022) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-022-04059-4 · Cited by 12
Systematic metabolic characterization of mental disorders reveals age‐related metabolic disturbances as potential risk factors for depression in older adults
Liu, Y., Zhao, W., Lu, Y., Zhao, Y., Zhang, Y., Dai, M., Hai, S., Ge, N., Zhang, S., Huang, M., et al. (2022) · MedComm · doi:10.1002/mco2.165 · Cited by 10
Physiological and Genetic Regulation for High Lipid Accumulation by Chlorella sorokiniana Strains from Different Environments of an Arctic Glacier, Desert, and Temperate Lake under Nitrogen Deprivation Conditions
Zou, S., Huang, Z., Wu, X., & Yu, X. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.00394-22 · Cited by 13
“The total lipid content was measured after 120-mL algal cultures at late logarithmic growth phase were harvested using a modified version of the Bligh and Dyer method (54).”
Method
Effect of Previous Frozen Storage, Canning Process and Packing Medium on the Fatty Acid Composition of Canned Mackerel
Prego, R., Trigo, M., Martínez, B., et al. (2022) · Marine Drugs · doi:10.3390/md20110666 · Cited by 10
Galactosyl- and glucosylsphingosine induce lysosomal membrane permeabilization and cell death in cancer cells
Stahl-Meyer, K., Bilgin, M., Holland, L. K. K., Stahl-Meyer, J., Kirkegaard, T., Petersen, N. H. T., Maeda, K., & Jäättelä, M. (2022) · PLoS ONE · doi:10.1371/journal.pone.0277058 · Cited by 10
“Lipids were extracted from a suspension of 200,000 cells or from purified lysosomes suspended in 200 μL 155 mM ammonium bicarbonate using a modified Bligh and Dyer protocol [38, 39].”
Method
Study on quality-based protocol for meat and meat products
Shin, D., Kim, T., Lee, J. H., Kim, B., Cha, J. Y., & Choi, Y. (2022) · Food and Life · doi:10.5851/fl.2022.e9 · Cited by 10
“식품 내 지방 추출법으로는 Bligh 와 Dyer법(Bligh and Dyer, 1959), Folch법(Folch et al., 1951) 및 Soxhlet 추출법(Soxhlet, 1879) 등이 있으며, 식육의 경우 Folch법과 Soxhlet 추출법이 주로 이용된다(Saini et al., 2021).”
Beyond the Interface: Improved Pulmonary Surfactant-Assisted Drug Delivery through Surface-Associated Structures
García-Mouton, C., Echaide, M., Serrano, L. A., et al. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15010256 · Cited by 10
Effect of packaging method and storage temperature on the sensory quality and lipid stability of fresh snakehead fish (Channa striata) fillets
Nguyen, M. V., KARNUE, S., & Kakooza, D. (2023) · Food Science and Technology · doi:10.1590/fst.116222 · Cited by 10
Plant–soil interactions in the native range of two congeneric species with contrasting invasive success
Florianová, A., Hanzelková, V., Drtinová, L., et al. (2023) · Oecologia · doi:10.1007/s00442-023-05329-6 · Cited by 10
“The PLFA were extracted from 1 g of freeze-dried soil samples with a mixture of chloroform–methanol-phosphate buffer (1:2:0.8, v/v/v), as previously described by Bligh and Dyer (1959).”
MethodInfluential
Raspberry Extract as a Strategy to Improve the Oxidative Stability of Pork Burgers Enriched with Omega-3 Fatty Acids
de Oliveira, A. S., dos Santos, B. A., Farias, C. A. A., et al. (2023) · Foods · doi:10.3390/foods12081631 · Cited by 10
“Lipid content was extracted and quantified using the Bligh and Dyer [15] method.”
Method
Regulation of Free Radical Processes by Delta-Sleep Inducing Peptide in Rat Tissues under Cold Stress
Shustanova, T. A., Бондаренко, Т. И., Milyutina, N. P., et al. (2001) · Biochemistry (Moscow) · doi:10.1023/a:1010255230338 · Cited by 10
Ultrastructure and lipid chemistry of specialized epidermal structure of Indian porcupines and hedgehog
Sarkar, M. P., Raha, P., Bhar, R., et al. (2010) · Acta Zoologica · doi:10.1111/j.1463-6395.2010.00452.x · Cited by 10
Transfer of the phosphatidyl moiety of phosphatidylglycerol to phosphatidylethanolamine in Escherichia coli
Yokota, K., & Kito, M. (1982) · Journal of Bacteriology · doi:10.1128/jb.151.2.952-961.1982 · Cited by 10
Enhanced triacylglyceride extraction from microalgae using free nitrous acid pre-treatment
Bai, X., Schenk, P. M., Yuan, Z., Lant, P. A., & Pratt, S. (2015) · Applied Energy · doi:10.1016/j.apenergy.2015.04.045 · Cited by 11
Osmoadaptative accumulation of N ɛ-acetyl-β-lysine in green sulfur bacteria and Bacillus cereus CECT 148T
Triadó–Margarit, X., Vilá, X., & Galinski, E. A. (2011) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2011.02254.x · Cited by 10
Turnover rates of fatty acid and amino acid in the coelomic fluid of the sea star Asterias rubens: Implications for the route of nutrient translocation during vitellogenesis
Beijnink, F. B., van der Sluis, I., & Voogt, P. A. (1984) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(84)90131-7 · Cited by 10
Determination of Freshness and Chemical Compositon of Scad ( Trachurus trachurus, L. 1758) Stored in Refrigerator
Şengör, G. F. Ü., Çelik, U., & Akkuş, S. (2000) · DergiPark (Istanbul University) · Cited by 10
The Effects of Dietary Fatty Acids on the Fatty Acid Composition in the Phospholipid Fraction of Gambusia affinis
Cengiz, E. İ., Ünlü, E., & Başhan, M. (2003) · DergiPark (Istanbul University) · Cited by 10
Characterization of the phospholipid and fatty acid composition of Sendai virus.
Barnes, J. A., Pehowich, D. J., & Allen, T. M. (1987) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38714-9 · Cited by 10
Sphingosine kinase A is a pleiotropic and essential enzyme for L eishmania survival and virulence
Zhang, O., Hsu, F., Xu, W., Pawlowic, M. C., & Zhang, K. (2013) · Molecular Microbiology · doi:10.1111/mmi.12378 · Cited by 10
The expression of the Cuphea palustris thioesterase CpFatB2 in Yarrowia lipolytica triggers oleic acid accumulation
Stefan, A., Hochkoeppler, A., Ugolini, L., et al. (2015) · Biotechnology Progress · doi:10.1002/btpr.2189 · Cited by 10
Phospholipase in the lysosomes of HEp-2 cells and its release during poliovirus infection
Rice, J. M., & WOLFF, D. A. (1975) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(75)90184-1 · Cited by 10
The biosynthesis and turnover of lipid during the differentiation of Dictyostelium discoideum
MacDonald, J. I., & Weeks, G. (1985) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(85)90002-5 · Cited by 10
CARACTERIZAÇÃO FÍSICA, FÍSICO-QUÍMICA E POTENCIAL TECNOLÓGICO DE FRUTOS DE CAMAPÚ (Physalis angulata L.)
de Oliveira, J. A. R., Martins, L. H. D. S., de Vasconcelos, M. A. M., et al. (2011) · Revista Brasileira de Tecnologia Agroindustrial · doi:10.3895/s1981-36862011000200009 · Cited by 10
Integrating community structure and stable isotope analysis to assess a heavily exploited coastal marine ecosystem off Central Vietnam
Nam, N. T. H., Miller, T. W., Huan, N. X., et al. (2011) · Fisheries Research · doi:10.1016/j.fishres.2011.04.014 · Cited by 10
Rapid characterization of lipids by MALDI MS. Part 1: Bacterial taxonomy and analysis of food oils
Lay, J. O., Gidden, J. A., Liyanage, R., et al. (2012) · Lipid Technology · doi:10.1002/lite.201100162 · Cited by 10
Association between very long chain fatty acids in the meibomian gland and dry eye resulting from n-3 fatty acid deficiency
Tanaka, H., Harauma, A., Takimoto, M., et al. (2015) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/j.plefa.2015.02.004 · Cited by 10
EFECTO DEL TIPO DE MÚSCULO SOBRE PARÁMETROS DE CALIDAD EN CARNE FRESCA DE CERDO IBÉRICO EFFECT OF MUSCLE TYPE ON QUALITY PARAMETERS OF FRESH MEAT FROM IBERIAN PIG EFECTO DO TIPO DE MÚSCULO SOBRE OS PARÁMETROS DA CALIDADE NA CARNE FRESCA DO PORCO IBÉRICO
Muriel, E., Antequera, T., & Ruiz, J. (2002) · Ciencia y Tecnologia Alimentaria · doi:10.1080/11358120209487735 · Cited by 10
Chryseomicrobium aureum sp. nov., a bacterium isolated from activated sludge
Deng, S.-K., Ye, X., Chu, C., et al. (2014) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.061143-0 · Cited by 10
Technological suitability of mutton for meat cured products
Beriain, M. J., Iriarte, J., Gorraiz, C., et al. (1997) · Meat Science · doi:10.1016/s0309-1740(97)00057-0 · Cited by 10
Sediment Organic Matter and its Nutritional Quality: A Short-Term Experiment with Two Exotic Bivalve Species
Mistri, M., Modugno, S., & Rossi, R. (2003) · Chemistry and Ecology · doi:10.1080/0275754031000119942 · Cited by 10
Changes in the Fatty Acid Composition of Hepatopancreas of the Mollusk Mytilus trossulus Fed on Microalgae
Khardin, A. S., Aizdaicher, N. A., & Латышев, Н. А. (2003) · Russian Journal of Marine Biology · doi:10.1023/b:rumb.0000011706.89867.ec · Cited by 10
Assessing the Myofibrillar and Connective Tissue Protein Contents and Protein Quality of Beef Tripe
Zarkadas, G. C., Karatzas, C. D., & Zarkadas, C. G. (1996) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf950262y · Cited by 10
A lysophosphatidic acid analogue is revealed as a potent inhibitor of phosphatidylcholine synthesis, inducing apoptosis
Guéguen, G., Granci, V., Rogalle, P., et al. (2002) · Biochemical Journal · doi:10.1042/bj20020273 · Cited by 10
Mechanism of the cytolytic action of Pseudomonas aeruginosa cytotoxin: Oligomerization of the cytotoxin on target membranes
Ohnishi, M., Hayashi, T., Tomita, N., et al. (1994) · FEBS Letters · doi:10.1016/0014-5793(94)01311-x · Cited by 10
Influence of sunflower oil on conjugated linoleic acid production byLactobacillus acidophilus andLactobacillus casei
Puniya, A. K., Reddy, C. S., Kumar, S., et al. (2009) · Annals of Microbiology · doi:10.1007/bf03175138 · Cited by 10
Effect of temperature and ions on the microviscosity of bilayers from natural phospholipid mixtures
Rossignol, M., Grignon, N., & Grignon, C. (1982) · Biochimie · doi:10.1016/s0300-9084(82)80493-8 · Cited by 10
Aerobic and anaerobic enzyme assays in Southern California Rockfish: Proxies for physiological and ecological data
Ombres, E. H., Donnelly, J., Clarke, M. E., et al. (2011) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/j.jembe.2010.11.007 · Cited by 10
3,5-Di-t-butyl-4-hydroxytoluene (BHT) and probucol stimulate selectively the reaction of mammalian 15-lipoxygenase with biomembranes
Schnurr, K., Kühn, H., Rapoport, S. M., & SCHEWE, T. (1995) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(94)00168-x · Cited by 10
Triacylglycerols and their fatty acid composition in edible Mediterranean molluscs and crustacean
Sinanoglou, V. J., Meimaroglou, D. M., & Miniadis‐Meimaroglou, S. (2008) · Food Chemistry · doi:10.1016/j.foodchem.2008.02.035 · Cited by 10

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks