Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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MethodInfluential
Evaluation of Biofloc Technology for Astacus leptodactylus: Effect of Different Stocking Densities on Production Performance and Physiological Responses
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High-quality Chlorella vulgaris biomass harvesting through chitosan and polyacrylamid2e
Wang, Y., Wang, J., Feng, C., Li, J., Wang, N., & Cai, J. (2022) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-17847-y · Cited by 6
“The lipid content of was measured using solvent extraction method and determined gravimetrically (Bligh and Dyer 1959).”
Method
Genome-Based Classification of Strain 16-SW-7, a Marine Bacterium Capable of Converting B Red Blood Cells, as Pseudoalteromonas distincta and Proposal to Reclassify Pseudoalteromonas paragorgicola as a Later Heterotypic Synonym of Pseudoalteromonas distincta
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Development of a High-Performance Thin-Layer Chromatography Method for the Quantification of Alkyl Glycerolipids and Alkenyl Glycerolipids from Shark and Chimera Oils and Tissues
Papin, M., Guimaraes, C., Pierre-Aue, B., et al. (2022) · Marine Drugs · doi:10.3390/md20040270 · Cited by 6
“NHP heart and tumors containing a high amount of membrane lipids were extracted according to a method adapted from Bligh and Dyer [37].”
Method
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Cardiolipin Biosynthesis Genes Are Not Required for Salmonella enterica Serovar Typhimurium Pathogenesis in C57BL/6J Mice
Cian, M. B., Mettlach, J. A., Zahn, A. E., Giordano, N. P., Minor, K., McClelland, M., & Dalebroux, Z. D. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.02617-21 · Cited by 6
Effect of Dietary Supplementation of Some Emulsifiers on Growth Performance, Carcass Traits, Lipid Peroxidation and Some Nutrients Digestibility in Broiler Chickens
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“Moreover ether extract was determined according to Bligh and Dyer (1959) technique as modified by Hanson and Olly (1963).”
Method
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NMR-Based Metabolomics of Rat Hippocampus, Serum, and Urine in Two Models of Autism
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“For analysis of hydrophobic compounds, the thawed 500- µl samples (urine or serum) were extracted for NMR spectrometry analysis using a mixture of methanol, chloroform, and HCl, according to a modified method of Bligh and Dyer [37], as previously described [30, 31].”
Method
Inhibition of anti-inflammatory pathway through suppressors of cytokine signalling (Socs2/Socs3) in the initiation of hepatocellular carcinoma
Ashfaq, I., Sheikh, N., Fatima, N., & Tayyeb, A. (2022) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2022.103348 · Cited by 6
“Hepatic lipid content was extracted according to modified Bligh and Dyer method (Bligh and Dyer 1959) as described previously (Perveen et al., 2020).”
Method
Effect of Lippia grata essential oil as a feed additive on the performance of tambatinga juveniles
Costa, T. S., da SILVA, R. C., Pretto, A., Monteiro, O. D. S., de Siqueira, J. C., Baldisserotto, B., & Lopes, J. M. (2022) · Acta Amazonica · doi:10.1590/1809-4392202102442 · Cited by 6
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Adição de farinha de casca de guavira em pão: características físico-químicas e sensoriais
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Phytotoxic, cytogenotoxic, and insecticidal activities of compounds from extracts of freshwater Lyngbya sp
da Silva, R. M. G., AMÂNCIO, I. F. N., de Andrade, A. R., dos Santos, V. H. M., Santiago, P. S., Granero, F. O., Figueiredo, C. C. M., & Silva, L. P. (2022) · Journal of Toxicology and Environmental Health · doi:10.1080/15287394.2022.2102100 · Cited by 6
“Total lipids of the extracts of Lyngbya sp. were extracted by the method described by Bligh and Dyer (1959) with some modifications.”
Method
Separation of triacylglycerols containing positional isomers of hexadecenoic acids by enantiomeric liquid chromatography-mass spectrometry
Řezanka, T., Lukavský, J., Rozmoš, M., Nedbalová, L., & Jansa, J. (2022) · Journal of Chromatography B · doi:10.1016/j.jchromb.2022.123401 · Cited by 6
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Liu, G., Bai, L., Sun, H., et al. (2022) · Italian Journal of Animal Science · doi:10.1080/1828051x.2022.2094290 · Cited by 6
“Total lipids were extracted according to Bligh and Dyer (1959) and directly transmethylated (Ichihara et al. 1996).”
Method
Inclusion of olive by-products in growing diets causes minor effects on meat quality of Iberian pigs fattened in a traditional system
Palma-Granados, P., Casco, J. M. G., Fernández‐Barroso, M. Á., López-García, A., Torres, J. M. M., Muñoz, M., & González, E. (2022) · Spanish Journal of Agricultural Research · doi:10.5424/sjar/2022203-19149 · Cited by 6
Microencapsulation of fish oil with olive leaf extract instead of synthetic antioxidant and its effects on nutraceutical properties of fish oil at different inlet temperatures
Özyurt, G., Durmuş, M., Özkütük, A. S., et al. (2022) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-022-03231-4 · Cited by 6
“The total oil was detected by the method of Bligh and Dyer [24].”

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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