Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Tüzün, N., Debecker, S., & Stoks, R. (2020) · Journal of Animal Ecology · doi:10.1111/1365-2656.13235 · Cited by 6
Targeted analysis of ceramides and cerebrosides in yellow lupin seeds by reversed-phase liquid chromatography coupled to electrospray ionization and multistage mass spectrometry
Bianco, M., Calvano, C. D., Losito, I., Palmisano, F., & Cataldi, T. R. I. (2020) · Food Chemistry · doi:10.1016/j.foodchem.2020.126878 · Cited by 6
Compositional differences, antioxidant activity, angiotensin-converting enzyme inhibition, and sensory characteristics of kamaboko fermented in wheat, rice, brown rice, and soybean koji
Nemoto, Y., Matsuo, Y., Shioda, K., et al. (2020) · Fisheries Science · doi:10.1007/s12562-020-01418-0 · Cited by 6
Regulation of mammary lipogenic genes in dairy cows fed crushed sunflower seeds
Lashkari, S., Moller, J. W., Theil, P. K., et al. (2020) · Livestock Science · doi:10.1016/j.livsci.2020.104035 · Cited by 6
Determination of poly‐β‐hydroxybutyrate assimilation by postlarval whiteleg shrimp, Litopenaeus vannamei using stable 13C isotope tracing
Ludevese-Pascual, G. L., Ahmed, F., Troch, M. D., et al. (2020) · Journal of the World Aquaculture Society · doi:10.1111/jwas.12700 · Cited by 6
“The freeze-dried shrimp samples were extracted using a modified Bligh–Dyer method (Bligh & Dyer, 1959; Rütters, Sass, Cypionka, & Rullkotter, 2002).”
Method
COLD AND HOT SMOKED NILE TILAPIA FILLETS: QUALITY AND YIELD OF PIGMENTED AND UNPIGMENTED FILLETS
de Souza, M. L. R., Viegas, E. M. M., Kronka, S. N., et al. (2020) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · doi:10.14674/ijfs-1567 · Cited by 6
“Aliquots from this sample were used for the chemical composition determinations (4 replications; moisture, ash, and crude protein) according to the official methodology of AOAC (2005), while total lipid determination was achieved based on the methodology described by BLIGH and DYER (1959).”
Method
Secagem do extrato de yacon (Smallanthus sonchifolius) por Spray Dryer: efeito dos diferentes agentes carreadores e avaliação dos teores de frutooligossacarídeos e compostos fenólicos
Asquieri, E. R., Nishi, A. C. F., Batista, R. D., & Asquieri, E. M. D. A. R. (2020) · Research Society and Development · doi:10.33448/rsd-v9i7.4521 · Cited by 6
Placental Trophoblast-Inspired Lipid Bilayers for Cell-Free Investigation of Molecular Interactions
Bailey‐Hytholt, C. M., Shen, T., Nie, B., Tripathi, A., & Shukla, A. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c06197 · Cited by 8
Effect of toasting and decortication of oat on rumen biohydrogenation and intestinal digestibility of fatty acids in dairy cows
Panah, F. M., Lashkari, S., Jensen, S. K., et al. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-18125 · Cited by 6
Enrichment diets of pigs with oil blends and its effects on performance, carcass characteristics and fatty acid profile
Souza, C. S., Moreira, J. A., Silva, N. R., et al. (2020) · Arquivo Brasileiro de Medicina Veterinária e Zootecnia · doi:10.1590/1678-4162-11106 · Cited by 6
Complete Replacement of the Galactolipid Biosynthesis Pathway with a Plant-Type Pathway in the Cyanobacterium Synechococcus elongatus PCC 7942
Apdila, E. T., Inoue, S., Shimojima, M., & Awai, K. (2020) · Plant and Cell Physiology · doi:10.1093/pcp/pcaa090 · Cited by 6
Aquirufa ecclesiirivi sp. nov. and Aquirufa beregesia sp. nov., isolated from a small creek and classification of Allopseudarcicella aquatilis as a later heterotypic synonym of Aquirufa nivalisilvae
Pitt, A., Koll, U., Schmidt, J., et al. (2020) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.004319 · Cited by 6
“Polar lipids and respiratory quinones were extracted and analysed as described by Tindall [11, 12] based on the method by Bligh and Dyer [13].”
Dietary fibre and protein do not synergistically influence insulin, metabolic or inflammatory biomarkers in young obese Göttingen minipigs
Xu, Y., Curtasu, M. V., Knudsen, K. E. B., Hedemann, M. S., Theil, P. K., & Lærke, H. N. (2020) · British Journal Of Nutrition · doi:10.1017/s0007114520003141 · Cited by 7
PINEAPPLE JAM PHYSICOCHEMICAL AND SENSORY EVALUATION WITH ADDED PINEAPPLE PEEL
dos Santos, B. A., Teixeira, F., Soares, J. M., do Amaral, L. A., de Souza, G. H. O., de Almeida, T. D. S. F., Schiessel, D. L., dos Santos, E. F., & Novello, D. (2020) · International Journal of Research -GRANTHAALAYAH · doi:10.29121/granthaalayah.v8.i7.2020.438 · Cited by 7
“The moisture (g 100 g-1), ash (g 100 g-1) and protein (g 100 g-1) contents were evaluated by the Association of Official Analytical Chemists [29] methodology; lipid (g 100 g-1) by Bligh and Dyer [31]; carbohydrate (g 100 g-1) by difference method (% Carbohydrate = 100 − (% moisture + % ash + % protein + % lipid + % fi…”
Method
Dietary effect of mead acid on DMBA‑induced breast cancer in female Sprague‑Dawley rats
Kinoshita, Y., Yoshioka, M., Emoto, Y., Yuri, T., Yuki, M., Koyama, C., Takenouchi, A., Hamazaki, K., Tsubura, A., & Yoshizawa, K. (2020) · International Journal of Functional Nutrition · doi:10.3892/ijfn.2020.7 · Cited by 6
“The fatty acid analysis of the total lipid content in the breast tissues was performed by a similar method as that described in the analysis of the serum and mammary glands, with the exception of excluding the separation step performed by thin‐layer chromatography (9,10,20).”
Method
Biodegradation of Hydrocarbon-Contaminated Drill Mud Waste with Compost and Cow Bile
Osei-Twumasi, D., Fei‐Baffoe, B., Anning, A. K., et al. (2020) · Environmental Processes · doi:10.1007/s40710-020-00464-1 · Cited by 6
“Total phospholipid concentration was analyzed by LAL-SOP-2 (Total Phospholipid and Fatty Acid Methyl Ester Composition) method (Bligh and Dyer 1959; Morrison and Smith 1964).”
Method
The effects of exogenous cerium on photosystem II as probed by in vivo chlorophyll fluorescence and lipid production ofScenedesmus obliquusXJ002
Cheng, J., Du, X., Long, H.-Y., Zhang, H., & Ji, X. (2020) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.2043 · Cited by 6
Haloferax profundi sp. nov. and Haloferax marisrubri sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea
Zhang, G., Dong, X., Sun, Y., et al. (2020) · Microorganisms · doi:10.3390/microorganisms8101475 · Cited by 6
“8, v/v/v), modified after Bligh and Dyer [31], and were recovered into the chloroform phase by adjusting the mixture to a ratio of 1:1:0.”
MethodInfluential

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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