Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Method
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Method
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Fassarella, L. B., Neto, J. G. O., Woyames, J., dos Santos, G. R. C., Pereira, H. M. G., Pazos-Moura, C. C., & Trevenzoli, I. H. (2024) · European Journal of Nutrition · doi:10.1007/s00394-024-03422-x · Cited by 6
“The total lipid of the liver was extracted using the Bligh and Dyer method [36], with some modifications.”
Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent
Jerez, H. E., Simioni, Y. R., Ghosal, K., Morilla, M. J., & Romero, E. L. (2024) · Beilstein Journal of Nanotechnology · doi:10.3762/bjnano.15.46 · Cited by 5
Enhancement of biomass production and bio-products extraction from microalgae using non-thermal plasma process toward biofuel production
Cubas, A. L. V., Moecke, E. H. S., Souza, L. D. P., Lima, A. S., Pereira, G. N., Dérner, R. B., & Matos, Â. P. (2024) · Brazilian Journal of Chemical Engineering · doi:10.1007/s43153-024-00456-w · Cited by 7
“The lipid content was determined according to Bligh and Dyer (1959), using cold extraction with a mixture of chloroform and methanol (2:1 v/v).”
Energy recovery of residual yeast via hydrothermal liquefaction with multi-cycle reuse of the post-HTL wastewater and subsequent anaerobic digestion
Sapillado, G., Zhang, Y., Summers, S., Ribeiro, R., & Tommaso, G. (2024) · Renewable Energy · doi:10.1016/j.renene.2024.121308 · Cited by 6
Efficacy of free glutathione and niosomal glutathione in the treatment of acetaminophen-induced hepatotoxicity in cats
Vulcano, L., author, N. A. N., Confalonieri, O. E., et al. (2013) · Open Veterinary Journal · doi:10.5455/ovj.2013.v3.i1.p56 · Cited by 6
Distinguishing wild and farm‐raised freshwater fish through fatty acid composition: Application of statistical tools
Carbonera, F., dos Santos, H. M. C., Montanher, P. F., et al. (2014) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201300339 · Cited by 6
Isolation and identification of microalgal strains and evaluation of their fatty acid profiles for biodiesel production.
Anitha, S. (2012) · International Journal of Pharmaceutical and Biological Archive · Cited by 6
The red microalga Rhodella reticulata as a source of a dietary ω-3 highly unsaturated fatty acid— Eicosapentaenoic acid
Yaron, A., Dvir, I., Maislos, M., et al. (1995) · Developments in food science · doi:10.1016/s0167-4501(06)80188-3 · Cited by 6
Dietary lipid sources affect freshwater catfish jundiá, Rhamdia quelen, survival, when challenged with Aeromonas hydrophila - doi: 10.4025/actascianimsci.v35i4.19617
Vargas, R. J., Dotta, G., Mouriño, J. L. P., et al. (2013) · Acta Scientiarum Animal Sciences · doi:10.4025/actascianimsci.v35i4.19617 · Cited by 6
Microalgal culture in photo-bioreactor for biodiesel production: case studies from Egypt
El-Ardy, O. A. M., El-Enin, S. A. A., Semary, N. A. H. E., & Diwani, G. E. (2012) · RACO (Revistes Catalanes amb Accés Obert) (Consorci de Serveis Universitaris de Catalunya) · Cited by 6
Effects of prefermented cereal-derived substrates (ground barley and rye bran) enriched with fungal γ-linolenic acid on rumen fermentation parameters and lipid metabolism in vitro
Laho, T., Váradyová, Z., Mihaliková, K., et al. (2011) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2011.05073.x · Cited by 6
In Situ Microcosm Studies to Characterize Microbial Processes in the Field
Kästner, M., & Richnow, H. H. (2009) · Springer eBooks · doi:10.1007/978-3-540-77587-4_273 · Cited by 6
Protein and lipid changes of FPC produced from Caspian Sea Kilkas in VP and MAP during storage at different temperatures
Khoshkhoo, Z., Motalebi, A. A., Razavilar, V., et al. (2012) · AquaDocs (United Nations Educational, Scientific and Cultural Organization) · Cited by 6
Cholesterol synthesis in central nervous system of rat is affected by simvastatin as well as by atorvastatin
Cibičková, Ľ., Hyšpler, R., Tichá, A., Cibicek, N., Palička, V., Čermáková, E., & Zadák, Z. (2008) · Die Pharmazie · doi:10.31083/ph.2008.8539 · Cited by 6
Changes in liver mitochondrial plasticity induced by brain tumor
Pouliquen, D., Olivier, C., Debien, E., et al. (2006) · BMC Cancer · doi:10.1186/1471-2407-6-234 · Cited by 6
Uptake of 12-HETE by human bronchial epithelial cells (HBEC): Effects on HBEC cytokine production
Gormand, F., Chabannes, B., Molière, P., et al. (1996) · Prostaglandins · doi:10.1016/0090-6980(96)00021-4 · Cited by 6
Pyrolytic characteristics of biodiesel prepared from lipids accumulated in diatom cells with growth regulation
Cheng, J., Feng, J., Ge, T., et al. (2015) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2014.12.010 · Cited by 6
Effect of recycling of fermentation broth for the production of penicillin amidase
Babu, P. S. R., & Panda, T. (1991) · Process Biochemistry · doi:10.1016/0032-9592(91)80002-7 · Cited by 6
Fatty Acids from the Sponge Tedania dirhaphis
Rodkina, S. A. (2005) · Chemistry of Natural Compounds · doi:10.1007/s10600-005-0131-x · Cited by 6
Evaluation of the high-pressure extrusion technique as a method for sizing plasmid DNA-containing cationic liposomes
Carstens, M. G., van der Maaden, K., van der Velden, D., Ottenhoff, T. H. M., Melief, C. J. M., OSSENDORP, F. A., Bouwstra, J. A., & Jiskoot, W. (2011) · Journal of Liposome Research · doi:10.3109/08982104.2011.563364 · Cited by 6
Rat Carboxylesterase ES-4 Enzyme Functions as a Major Hepatic Neutral Cholesteryl Ester Hydrolase
Parathath, S., Doğan, S., Joaquin, V. A., Ghosh, S., Guo, L., Weibel, G. L., Rothblat, G. H., Harrison, E. H., & Fisher, E. A. A. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.258095 · Cited by 6
Control ofAspergillus nigerinfection in varieties ofArachis hypogeaeL. by supplementation of zinc ions during seed germination
Jajda, H. M., & Thakkar, V. R. (2012) · Archives of Phytopathology and Plant Protection · doi:10.1080/03235408.2012.677312 · Cited by 6
Comparative Study on Hypericum triquetrifolium Turra Fatty Acids
Hosni, K., Msaâda, K., & Marzouk, B. (2007) · Asian Journal of Plant Sciences · doi:10.3923/ajps.2007.384.388 · Cited by 6
Liberation of free fatty acids during production of potato granules
Lilja, M., & Lingnert, H. (1989) · Food Chemistry · doi:10.1016/0308-8146(89)90029-0 · Cited by 6
Fontes protéicas vegetais na alimentação da carpa húngara
Bergamin, G. T., Martinelli, S. G., Flora, M. A. L. D., et al. (2011) · Ciência Rural · doi:10.1590/s0103-84782011000900028 · Cited by 6
ICAM-1 Upregulation in Ethanol-Induced Fatty Murine Livers Promotes Injury and Sinusoidal Leukocyte Adherence after Transplantation
Theruvath, T. P., Ramshesh, V. K., Zhong, Z., et al. (2012) · HPB Surgery · doi:10.1155/2012/480893 · Cited by 6
Comparative study of the cellular fatty acids of methicillin-resistant and -susceptibleStaphylococcus aureus
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Oral Administration of Docosahexaenoic Acid/Eicosapentaeinoic Acids Is Not Anticonvulsant in Rats: Implications for Translational Research
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Physicochemical properties of three sugary cassava landraces
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Cell culturing in a three‐dimensional matrix affects the localization and properties of plasma membrane cholesterol
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Phospholipid and fatty acid composition of frog (Rana esculenta) liver—a circannual study
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Capsule polysaccharide-protein-peptidoglycan complex in the cell envelope of Rhodobacter capsulatus
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Microbial Response to Organic Matter Enrichment in the Oligotrophic Levantine Basin (Eastern Mediterranean)
Gontikaki, E., Polymenakou, P., Thornton, B., et al. (2012) · Geomicrobiology Journal · doi:10.1080/01490451.2011.605822 · Cited by 6
Lipid composition ofbacillus megaterium spores and spore membranes
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Molecular analysis of a novel Drosophila diacylglycerol kinase, DGKϵ
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Lipid and fatty acid compositions of a trematode, Isoparorchis hypselobagri Billet, 1898 (Digenea: Isoparorchiidae) infecting swim bladder of Wallago attu in the district North 24-Parganas of West Bengal
Mondal, J., & Dey, C. (2013) · Journal of Parasitic Diseases · doi:10.1007/s12639-013-0283-8 · Cited by 6
Effects of muscle types and cooling on discoloration of canned skipjack
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Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus
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Ripening and Sensory Properties of Spice-Cured Sprats and Sensory Properties Development
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Fishmeal quality and ethoxyquin effects on the weaning performance of ballan wrasse (Labrus bergylta) larvae
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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