Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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Dunstan, G. A., Volkman, J. K., Barrett, S. M., LeRoi, J., & Jeffrey, S. W. (1993) · Phytochemistry · doi:10.1016/s0031-9422(00)90525-9 · Cited by 424
Lipid production of Chlorella vulgaris cultured in artificial wastewater medium
Feng, Y., Li, C., & Zhang, D. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.016 · Cited by 431
Carbon isotopic fractionation in heterotrophic microbial metabolism
Blair, N. E., Leu, A., Mu�oz, E., Olsen, J. V., Kwong, E., & Marais, D. D. (1985) · Applied and Environmental Microbiology · doi:10.1128/aem.50.4.996-1001.1985 · Cited by 426
Oxidized phosphatidylserine–CD36 interactions play an essential role in macrophage-dependent phagocytosis of apoptotic cells
Greenberg, M. E., Sun, M., Zhang, R., Febbraio, M., Silverstein, R., & Hazen, S. L. (2006) · The Journal of Experimental Medicine · doi:10.1084/jem.20060370 · Cited by 430
Cultured endothelial cells synthesize both platelet-activating factor and prostacyclin in response to histamine, bradykinin, and adenosine triphosphate.
McIntyre, T. M., Zimmerman, G. A., Satoh, K., et al. (1985) · Journal of Clinical Investigation · doi:10.1172/jci111957 · Cited by 427
Short-term CO2 and N2O emissions and microbial properties of biochar amended sandy loam soils
Ameloot, N., De Neve, S., Jegajeevagan, K., Yildiz, G., Buchan, D., Funkuin, Y. N., Prins, W., Bouckaert, L., & Sleutel, S. (2013) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2012.10.025 · Cited by 427
Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soil
Kandeler, E., Tscherko, D., Bruce, K. D., Stemmer, M., Hobbs, P. J., Bardgett, R. D., & Amelung, W. (2000) · Biology and Fertility of Soils · doi:10.1007/s003740000268 · Cited by 425
Telavancin, a Multifunctional Lipoglycopeptide, Disrupts both Cell Wall Synthesis and Cell Membrane Integrity in Methicillin-Resistant Staphylococcus aureus
Higgins, D. L., Chang, R., Debabov, D. V., Leung, J., Wu, T. X., Krause, K. M., Sandvik, E., Hubbard, J. M., Kaniga, K., Schmidt, D. E., et al. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.3.1127-1134.2005 · Cited by 426
Enhancement of calcium uptake and phosphatidylinositol turnover by epidermal growth factor in A-431 cells
Sawyer, S. T., & Cohen, S. (1981) · Biochemistry · doi:10.1021/bi00524a057 · Cited by 426
Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs
Lewis, T. E., Nichols, P. D., & McMeekin, T. A. (2000) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(00)00217-7 · Cited by 425
Determination of brominated flame retardants, with emphasis on polybrominated diphenyl ethers (PBDEs) in environmental and human samples—a review
Covaci, A. (2003) · Environment International · doi:10.1016/s0160-4120(03)00114-4 · Cited by 425
Mutations in SPTLC1, encoding serine palmitoyltransferase, long chain base subunit-1, cause hereditary sensory neuropathy type I
Dawkins, J. L., Hulme, D. J., Brahmbhatt, S., Auer‐Grumbach, M., & Nicholson, G. A. (2001) · Nature Genetics · doi:10.1038/85879 · Cited by 421
Phospholipid fatty acid profiling of microbial communities-a review of interpretations and recent applications
Willers, C., van Rensburg, P. J., & Claassens, S. (2015) · Journal of Applied Microbiology · doi:10.1111/jam.12902 · Cited by 428
Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1
Myers, C. R., & Myers, J. M. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.11.3429-3438.1992 · Cited by 423
Structure, mechanism, and regulation of the chloroplast ATP synthase
Hahn, A. S., Vonck, J., Mills, D. J., Meier, T. W., & Kühlbrandt, W. (2018) · Science · doi:10.1126/science.aat4318 · Cited by 424
Introduction of liposome-encapsulated SV40 DNA into cells.
Fraley, R. T., Subramani, S. E., Berg, P. R., & Papahadjopoulos, D. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70482-7 · Cited by 416
13,16-Dimethyl Octacosanedioic Acid ( iso -Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3
Damsté, J. S. S., Rijpstra, W. I. C., Hopmans, E. C., Weijers, J. W. H., Foesel, B. U., Overmann, J., & Dedysh, S. N. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.00466-11 · Cited by 420
CHEMICAL PROFILE OF SELECTED SPECIES OF MICROALGAE WITH EMPHASIS ON LIPIDS1
Ben‐Amotz, A., Tornabene, T. G., & Thomas, W. H. (1985) · Journal of Phycology · doi:10.1111/j.0022-3646.1985.00072.x · Cited by 420
Increased biosynthesis of platelet-activating factor in activated human eosinophils.
Lee, T., Lenihan, D. J., Malone, B., Roddy, L. L., & Wasserman, S. I. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91044-6 · Cited by 409
The Use of Sephadex for the Removal of Nonlipid Contaminants from Lipid Extracts*
Wells, M. A., & Dittmer, J. C. (1963) · Biochemistry · doi:10.1021/bi00906a015 · Cited by 419
Effects of chemical lipid extraction and arithmetic lipid correction on stable isotope ratios of fish tissues
Sweeting, C. J., Polunin, N. V. C., & Jennings, S. (2006) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.2347 · Cited by 418

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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