Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 41,501–41,600 of 48,847

The role of lipids in the nutrition of shredding aquatic insects
Cargill, A. S. (1984) · Cited by 4
Interactions of Alcohol and Prostanoids in the Vascular System
Karanian, J. W., & Salem, N. (1987) · Springer eBooks · doi:10.1007/978-1-4684-5296-9_35 · Cited by 4
Inhibitory activity of the serum from patients with fulminant hepatitis against liver regeneration
Tada, K., Akamatsu, K., & Ohta, Y. (1986) · Gastroenterologia Japonica · doi:10.1007/bf02774565 · Cited by 4
PREHISTORIC DIET IN CENTRAL BA JA CALIFORNIA, MEXICO
King, J. (1997) · Library and Archives Canada (Government of Canada) · Cited by 4
The Effect of Polar Thylakoid Lipids on Oxygen Evolution
Gounaris, K., Whitford, D., & Barber, J. P. (1984) · Springer eBooks · doi:10.1007/978-94-017-4973-2_23 · Cited by 4
Loss-of-function mutations in novel triacylglycerol lipase genes are associated with low rancidity in pearl millet flourPreprint
Aher, R. R., Reddy, P. S., Bhunia, R. K., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.04.02.486827 · Cited by 4
“Quantification of Lipids by GC-FID and HPLC-ELSD The lipid extractions were based on the method of Bligh and Dyer (1959).”
Method
Studies on the deoxyribonucleic acid-bearing portion of the cell envelope ofEscherichia coli
Weisshäupl, V., & Dworsky, P. (1978) · Journal of Basic Microbiology · doi:10.1002/jobm.3630180907 · Cited by 4
Arterial prostaglandins and lysosomal function during atherogenesis
Berberian, P. A., & Jenison, M. W. (1985) · Experimental and Molecular Pathology · doi:10.1016/0014-4800(85)90052-8 · Cited by 4
Derivatization in Gas Chromatography of Lipids
Řezanka, T., Pádrová, K., & Sigler, K. (2016) · Springer eBooks · doi:10.1007/978-94-007-7864-1_71-1 · Cited by 4
The Evaluation of Neurodevelopmental and Behavioural Correlates of Acute Postnatal Exposure to di(2-ethylhexyl) phthalate in Rats
Smith, C. (2014) · Carleton University's Institutional Repository (MacOdrum Library, Carleton University) · doi:10.22215/etd/2014-10590 · Cited by 4
Irreversible Deacylation of Plasma Membrane Phospholipids by the Combined Action of Mg2+ and a Long-Chain Acyl-CoA Synthetase Inhibitor in Saccharomyces cerevisiae
NAKAYAMA, K., Nakamura, T., Taniguchi, M., et al. (2002) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.94.258 · Cited by 4
STRUCTURE AND COMPOSITION OF OOCYSTIS POLYMORPHA CELL WALLS ISOLATED FROM THE CULTURE MEDIUM1
Schwertner, H. A., Butcher, W. I., & Richardson, B. (1972) · Journal of Phycology · doi:10.1111/j.0022-3646.1972.00144.x · Cited by 4
Knockout of cardiolipin synthase disrupts postnatal cardiac development by inhibiting the maturation of mitochondrial cristaePreprint
Ren, M., Yang, X., Phoon, C. K. L., Erdjument‐Bromage, H., Neubert, T. A., & Schlame, M. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.03.09.531996 · Cited by 4
“Lipidomics Samples, containing about 1 mg cardiac protein, were extracted into chloroform/methanol as described (Bligh & Dyer, 1959).”
MethodInfluential
Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in Monoraphidium sp. for Pharmacological and Nutraceutical ApplicationsPreprint
Yadav, K., Kumar, S. B., Nikalje, G. C., & Rai, M. P. (2023) · Preprints.org · doi:10.20944/preprints202303.0382.v1 · Cited by 4
Defective pgsA contributes to increased membrane fluidity and cell wall thickening in S. aureus with high-level daptomycin resistancePreprint
Freeman, C. D., Hansen, T., Urbauer, R. J. B., Wilkinson, B. J., Singh, V. K., & Hines, K. M. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.04.11.536441 · Cited by 4
“A modified version of the Bligh and Dyer method was used for the lipid extraction (30).”
Method
FGF-21 Conducts a Liver-Brain-Kidney Axis to Promote Renal Cell CarcinomaPreprint
Li, Z., Zhang, X., Zhu, W., Zhang, C., Sadak, K., Halberstam, A. A., Brown, J. R., Perry, C. J., Bunn, A., Braun, D. A., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.04.12.536558 · Cited by 5
Molecular Basis of Cell Membrane Adaptation in Daptomycin-Resistant Enterococcus faecalisPreprint
Nguyen, A. H., Tran, T. T., Panesso, D., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.08.02.551704 · Cited by 4
Multi-OMIC analysis of Huntington disease reveals a neuroprotective astrocyte statePreprint
Paryani, F., Kwon, J., Ng, C. W., Madden, N., Ofori, K., Tang, A., Lü, H., Li, J., Mahajan, A., Davidson, S. M., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.09.08.556867 · Cited by 4
Dataset representing growth performance, nutritional assay and biochemical profile of Oscillatoria spp.
Nayeem, J., Dey, P., Dey, S. K., et al. (2024) · Data in Brief · doi:10.1016/j.dib.2024.110255 · Cited by 4
“The lipid content of the samples was determined according to the Bligh and Dyer [12] and Folch et al. (1957) [13] methods.”
Method
Comparative dataset on productivity, proximate, biochemical and pigment content of marine Gonyostomum sp. and freshwater Tetraedron sp. microalgae
Dey, P., Nayeem, J., Dey, S. K., et al. (2024) · Data in Brief · doi:10.1016/j.dib.2024.110393 · Cited by 4
Expanding the cultivable human archaeome: Methanobrevibacter intestini sp. nov. and strain Methanobrevibacter smithii “ GRAZ-2 ” from human fecesPreprint
Weinberger, V., Mohammadzadeh, R., Blohs, M., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.05.15.594450 · Cited by 4
Bam complex associated proteins in Escherichia coli are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replicationPreprint
Bryant, J. A., Staunton, K. A., Doherty, H. M., Alao, M. B., Ma, X., Morcinek-Orłowska, J., Goodall, E. C. A., Gray, J., Milner, M., Cole, J. A., et al. (2024) · eLife · doi:10.7554/elife.99955.1 · Cited by 7
Molecular basis for multidrug efflux by an anaerobic RND transporterPreprint
Lawrence, R., Athar, M., Uddin, M. R., Adams, C., Sousa, J. S., Durrant, O., Lellman, S., Sutton, L., Keevil, C. W., Patel, N., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.04.04.646765 · Cited by 4
“For lipidomics and GC-MS experiments, lipids were extracted according to a modified version of Bligh and Dyer 61 .”
Oxidation of marine phospholipids in liposomes
Mozūraitytė, R. (2007) · BIBSYS Brage (BIBSYS (Norway)) · Cited by 4
Changes in parameters of mechanics of breathing during high-frequency jet ventilation: what is the cause?
Čalkovská, A., Javorka, K., Petraskova, M., et al. (1997) · PubMed · Cited by 4
Low-lying agricultural peatland sustainability under managed water regimes
Dawson, Q. (2006) · OpenGrey (Institut de l'Information Scientifique et Technique) · Cited by 4
[16] Penicillinase secretion in Vivo and in Vitro
NIELSEN, J. B. K. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)97129-x · Cited by 4
Effective bioprocess engineering to enhance omega‐6 polyunsaturated fatty acid production from Arthrospira platensis
Dubey, S., Singhania, R. R., Ramanujam, P. K., Chen, C., Dong, C., & Patel, A. K. (2025) · Physiologia Plantarum · doi:10.1111/ppl.70186 · Cited by 3
“For this experiment, 100 mg of dry biomass of Arthrospira was placed into a 15 mL centrifuge tube for lipid estimation, following the method outlined by Bligh and Dyer (Bligh & Dyer, 1959).”
Visualization and quantification of oil in single microalgal cells
Al Ahmad, M., Al-Zuhair, S., Hilal-Alnaqbi, A., & Mustafa, F. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-016-1002-5 · Cited by 3
Outdoor Closed System of Algal Mass Culture : In Sight of Comparison on Vertical and Horizontal Photobioreactor for Cultivating the Spirulina sp.
Farida, H., Harahap, P. S., Sobari, R., Gunawan, R., Rahman, D. Y., & Susilaningsih, D. (2019) · REAKTOR · doi:10.14710/reaktor.19.2.54-61 · Cited by 3
An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica
Zhang, Z., Liu, H., Pan, X., Zong, Y., Feng, L., Liu, L., Guo, L., & Yang, G. (2023) · Journal of Oceanology and Limnology · doi:10.1007/s00343-023-2332-1 · Cited by 3
“Total lipids were extracted from about 100 mg of lyophilized biomass with the chloroform-methanol solvent mixture (2 ꞉ 1, v/v) using a procedure described previously by Bligh and Dyer (1959).”
Metabolomics in Natural Product Discovery and Their Applications
Nath, S., & Gomes-Dias, L. (2023) · Springer eBooks · doi:10.1007/978-3-031-39094-4_9 · Cited by 5
Plasma S1P Orchestrates the Reverse Transendothelial Migration of Aortic Intimal Myeloid Cells in Mice
Polenz, C. K., Scipione, C. A., Hyduk, S. J., Althagafi, M. G., Ibrahim, H. M., & Cybulsky, M. I. (2024) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.123.320227 · Cited by 3
Sugar beet molasses: a sweet solution for ectoine production by Nesterenkonia sp.
Orhan, F., & Ceyran, E. (2024) · Environmental Science and Pollution Research · doi:10.1007/s11356-024-34674-z · Cited by 4
“We employed a previously described method (Bligh and Dyer 1959) to extract ectoine from the cell pellets.”
Droughts Legacy Effects on Phosphorus Transformation from Residues and Mineral Fertilizers in Calcareous and Carbonate-Free Soils: A 33P Labeling Study
Farooq, N., Koirala, M., Loftus, S., et al. (2024) · Journal of soil science and plant nutrition · doi:10.1007/s42729-024-02120-1 · Cited by 3
“Briefly, 6 g of soil was extracted twice using a Bligh/Dyer solution, a mixture of chloroform, methanol, and citrate buffer (0.8:1:2, v: v: v) (Bligh and Dyer 1959).”
Influential
Delta‐9 desaturase reduction in gastrointestinal cells induced to senescence by doxorubicin
Nunzio, V. D., Caruso, E. A., Centonze, M., Pinto, G., Cofano, M., Saponara, I., & Notarnicola, M. (2024) · FEBS Open Bio · doi:10.1002/2211-5463.13945 · Cited by 3
“Cellular lipids were extracted using a modiﬁed Bligh and Dyer method with chloroform/methanol/water (2:1:1.8; v/v/v) [34].”
Influential
Seasonal Water-Column Structure Drives the Trophic Niche of Fish Communities on a Temperate Continental Shelf
Kundu, G. K., Kim, C., Jang, J., et al. (2024) · Biology · doi:10.3390/biology13121041 · Cited by 3
Transcriptomic Signature of Lipid Production in Australian Aurantiochytrium sp. TC20
Chang, K. J. L., Gomez, E. G., Ebrahimie, E., Dehcheshmeh, M. M., Frampton, D. M. F., & Zhou, X. (2025) · Marine Biotechnology · doi:10.1007/s10126-025-10415-2 · Cited by 3
“Freeze-dried biomass (100 mg) was extracted using a modified version of Bligh and Dyer’s method, involving a mixture of dichloromethane (DCM), methanol (MeOH), and water (Bligh and Dyer 1959).”
Comprehensive lipidomics analysis of Cape hake (Merluccius capensis) by-products: Screening key features for added value applications
Monteiro, J. P., Sousa, T., Ferreira, H., Pinho, M., Melo, T., Goracci, L., Pires, C., Marques, A., Nunes, M. L., Domingues, P. M., et al. (2025) · Applied Food Research · doi:10.1016/j.afres.2025.100839 · Cited by 4
Nutritional composition and energetic values of mesopelagic fish from the Tasman Sea
Zhang, B., Virtue, P., Pethybridge, H., Swadling, K. M., Nichols, P. D., & Chang, K. J. L. (2025) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2025.107509 · Cited by 4
The Cat's Whiskers: Stable Isotopes Reveal Individual Specialisation of Adaptable Caracals (Caracal caracal) Foraging in an Urbanising Landscape
Leighton, G. R. M., Brooke, A. R., Froneman, P. W., Serieys, L. E. K., & Bishop, J. M. (2025) · Ecology and Evolution · doi:10.1002/ece3.71154 · Cited by 3
“Sectioned whiskers were degreased for 24 h using a cleaning solution of chloroform, methanol, and distilled water in a ratio of 2:1:0.8, as described in Bligh and Dyer (1959) and following Lee-Thorp et al. (1989).”
Influential
Precursor direct biosynthesis of odd-chain polyunsaturated fatty acids by oomycete Pythium
Palyzová, A., Kulišová, M., Majtán, K., Fous, K., Kolouchová, I., & Řezanka, T. (2025) · Food Chemistry · doi:10.1016/j.foodchem.2025.144204 · Cited by 3
The effect of genotype and housing system (deep litter and enriched cages) on physical characteristics and chemical compositions of eggs from layer breeders
Şekeroǧlu, A., Tainika, B., Özer, C. O., et al. (2025) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2025.107624 · Cited by 3
Comparative Evaluation of Chlorella v-ulgaris and Chlorella sorokiniana for Food Related Applications
Georgiou, D., Charisis, A., Exarhopoulos, S., Papapanagiotou, G., Samara, C., Chatzidoukas, C., & Kalogianni, E. P. (2025) · Waste and Biomass Valorization · doi:10.1007/s12649-025-03042-4 · Cited by 5
“The lipid recovery was determined by comparing the lipid yield obtained through the aforementioned methods to the yield achieved using the Bligh and Dyer method [34]. with the content in Monoraphidium sp [24]. and exceeded levels found in most commonly consumed vegetable oils [53].”
Endogenous formation of phosphatidylhomoserine in Escherichia coli through phosphatidylserine synthase
Ng, E. Z. Q., Lee, E.-J., Chng, S., Kim, J., & Guan, X. L. (2025) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2025.110255 · Cited by 4
Alkylglycerol monooxygenase represses prostanoid biosynthesis in a sex-dependent manner
Rao, Z., Lackner, K., Dorigatti, I., et al. (2025) · Cell & Bioscience · doi:10.1186/s13578-025-01419-5 · Cited by 3
“Following centrifugation (3,000 × g, 5 min, 4 °C), the chloroform layer was collected and brought to dryness using a SpeedVac vacuum concentrator [42].”
Spray-drying of chickpea aquafaba with biopolymers: Improving physical and techno-functional properties for meringue production
Silva, M. P., Semedo, J. M., Toledo, C. C. D. S., et al. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.145426 · Cited by 3
Mapping the oxidative landscape in cystic fibrosis: methodological frontiers and application
Rubin, M., Artusi, I., & Cozza, G. (2025) · Frontiers in Pharmacology · doi:10.3389/fphar.2025.1632924 · Cited by 5
“The Bligh and Dyer method represents the predominant approach, employing chloroform-methanol solvent systems to effectively solubilize and recover diverse lipid classes across polarity ranges (Bligh and Dyer, 1959; Ollero et al., 2011).”
Stearidonic Acid-Enriched Buglossoides arvensis Oil Alleviates High-Fat Diet-Induced Hepatic Lipid Accumulation and Inflammation via AMPK/SIRT1 Activation in Rats
Lee, M.-S., Kim, B., Lee, J., Cho, Y., Kim, I.-H., & Kim, Y. (2025) · Journal of Nutrition · doi:10.1016/j.tjnut.2025.08.005 · Cited by 3
The Evolutionary History and Modern Diversity of Triterpenoid Cyclases
McShea, H. S., Viens, R. A., Olagunju, B. O., Giner, J., & Welander, P. V. (2025) · Molecular Biology and Evolution · doi:10.1093/molbev/msaf203 · Cited by 3
“Cell pellets were extracted using a modified Bligh–Dyer method (Bligh and Dyer 1959; Welander et al. 2012): cells were re-suspended in 2 mL of deionized water and transferred to a solvent-washed Teflon centrifuge tube containing 5 mL of methanol and 2.5 mL of dichloromethane (DCM), vortexed for 30…”
Influential
Effect of ultrasound pre-treatments before enzymatic hydrolysis on physicochemical, nutritional and functional properties of insoluble protein fractions obtained from Atlantic mackerel side streams
Cropotova, J., Kvangarsnes, K., Kotsoni, E., et al. (2025) · Bioresources and Bioprocessing · doi:10.1186/s40643-025-00931-3 · Cited by 3
Bio‐Based Strategies for Sustainable Packaging: Incorporation of Babassu Mesocarp Flour Into Poly(Lactic Acid) Films
da Silva, L. R. C., Chaves, R. P. F., Pena, R. D. S., Alves, T. S., Barbosa, R., Köhn, C. R., Rios, A. D. O., & Santana, R. M. C. (2025) · Polymers for Advanced Technologies · doi:10.1002/pat.70334 · Cited by 3
“Total lipids were quantified using the method of Bligh and Dyer [28], while total fiber was analyzed by Prosky et al. [29].”
Downstream processes for yeast microbial oils extraction
de Vicente, M., González‐Fernández, C., & Tomás‐Pejó, E. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-30188-9.00008-1 · Cited by 3
Optimizing algae-bacteria interaction mode to enhance resource recovery from landfill leachate
Nie, Y., Chang, H., Liang, C., et al. (2025) · Chemical Engineering Journal · doi:10.1016/j.cej.2025.167967 · Cited by 3
A bibliometric analysis and evaluation of publications published in the meat science journal from 1980 to 2025
Özdemir, M., Hopkins, D. L., Öz, E., et al. (2025) · Meat Science · doi:10.1016/j.meatsci.2025.109952 · Cited by 3
Obtaining and characterization of flour and oil from black soldier fly (Hermetia illucens) and yellow mealworm (Tenebrio molitor) larvae for human consumption
da Silva, N. M., Bevilaqua, G. C., da Silva, C., et al. (2025) · Applied Food Research · doi:10.1016/j.afres.2025.101359 · Cited by 3
Potential limits to local adaptation of Coho Salmon populations from eggs to fry under naturally varying and warming temperature conditions
Iacarella, J. C., Chea, R., & Patterson, D. A. (2025) · Transactions of the American Fisheries Society · doi:10.1093/tafafs/vnaf039 · Cited by 3
Towards sustainable lipidomics: computational screening and experimental validation of chloroform-free alternatives for lipid extraction
Venturi, A., Wölk, M., Penkov, S., Cruciani, G., Fedorova, M., & Goracci, L. (2025) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-025-06136-z · Cited by 4
VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair
Adeosun, O. A., Schröer, C., Südhoff, E., Bergenthal, F., Sommer, K., Döffinger, E., Fiedler, B., Hilderink, A., Lehmann, A., Pohle, L.-S., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-75145-y · Cited by 5
“Lipid extraction was performed according to the Bligh-Dyer method 64 .”
Amino acid metabolites as potential circulating biomarkers for sarcopenia
Seo, J. H., Koh, J., Kim, S. J., et al. (2025) · Scientific Reports · doi:10.1038/s41598-025-24223-0 · Cited by 3
Endothelial CEPT1 Promotes Angiogenesis Through PPARα and VEGF-A Signaling
Khan, T. J., Meade, R., Elizondo-Benedetto, S., Belaygorod, L., Saffaf, O., Rusconi, B. A., Hsu, F., Adak, S., Arif, B., Zaghloul, M. S., et al. (2025) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.125.323302 · Cited by 3
Lipid provisioning in eggs of an extreme dietary generalist
Sellers, E. J., Hicks, K. G., Tanino-Springsteen, M., Vidal, M. C., & Murphy, S. M. (2025) · Physiological Entomology · doi:10.1111/phen.70019 · Cited by 4
“…differently across host plants, future research could incorporate more precise methods of lipid differentiation and sample homogenization such as described in previous works to identify various lipid types (Bligh & Dyer, 1959; Folch et al., 1957; methodologies compared by Axelsson & Gentili, 2014).”
Flax lignan-fortified nanoemulsions potentiate the conversion of α-linolenic acid to n-3 LCPUFAs: cumulative metabolic patterns in non-fasting mice
Wang, L., Yu, X. L., Cheng, C., et al. (2025) · Food & Function · doi:10.1039/d5fo03469h · Cited by 3
Unraveling anti-inflammatory metabolic signatures of Glycyrrhiza uralensis and isoliquiritigenin through multiomics
Kiuchi, S., Chung, M. H., Sakai, H., Nakaya, T., Ohbuchi, K., Tsumagari, K., Imami, K., Otoguro, Y., Nitta, T., Yamamoto, H., et al. (2025) · npj Systems Biology and Applications · doi:10.1038/s41540-025-00620-z · Cited by 3
Dietary lipid droplet size and phospholipids modulate lipid digestion and absorption in early life nutrition, an in vitro study
Thomassen, G. G. M., Penhoat, A., van den Braak, C., Durand, A., Chanon, S., Renes, I. B., Abrahamse, E., & Michalski, M. (2025) · Food Chemistry · doi:10.1016/j.foodchem.2025.147742 · Cited by 3
Fe(Urea) nanocomposite-driven metabolic modulation for enhancing lutein and biofuel precursors in microalgae
Sumathi, Y., Kumar, P., & Patel, A. K. (2025) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2025.120916 · Cited by 3
Comparative evaluation of sludge characteristics and methane potential from different municipal wastewater treatment processes
Çiçekalan, B., Kosar, S., Buyukbuberoglu, F. B., et al. (2026) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2026.108932 · Cited by 3
Nutritional Composition of Seven Selected Seaweed Species Commonly Available in Bangladesh: A Systematic Review and Meta‐Analysis
Naoar, N., Islam, M. S., Sultana, F., Asaduzzaman, M., Rafiquzzaman, S. M., Hannan, A., Jahan, N., & Mamun, A. (2026) · eFood · doi:10.1002/efd2.70124 · Cited by 3
Abiotic Extracellular Catalysis Plays an Underappreciated Role in Soil Carbon Decomposition across Terrestrial Ecosystems
Zhao, Y., Liu, C., Zhang, W., et al. (2026) · Environmental Science & Technology · doi:10.1021/acs.est.5c13520 · Cited by 3
Pulmonary iron oxide (Fe 3 O 4 ) nanoparticle-biomolecule interactions modified during aging and metabolic syndrome disease progression
Shinde, A., Xia, L., Thirumalaikumar, V. P., Ferreira, C. G., Lanman, N., & Shannahan, J. H. (2026) · Human & Experimental Toxicology · doi:10.1177/09603271261418788 · Cited by 3
“46 47–50 Specifically, isolated NP-BCs were resuspended in 200 μL water followed by adding 125 μL of chloroform and 225 μL of methanol.”
High-pressure processing combined with chitooligosaccharide-catechin conjugate enhances quality and shelf-life of precooked crab meat
Pimsannil, K., Palamae, S., Fu, Y., et al. (2026) · Food Bioscience · doi:10.1016/j.fbio.2026.108346 · Cited by 3
Physicochemical Characterization of Potato Peel Flour (Solanum tuberosum L.) and Optimization of the Ultrasound-Assisted Extraction Process (UAE) of the Phenolic Compounds Using Response Surface Methodology
Batista, R. D., Asquieri, E. R., Silva, A. G. D. M. E., et al. (2026) · Potato Research · doi:10.1007/s11540-025-09997-0 · Cited by 3
“For this, the samples were defatted according to Bligh and Dyer (1959), dried in an oven (model 402/D, Ethik Technology brand, Vargem Grande Paulista, SP, Brazil) at 45 °C, and the morphology was evaluated at magnifications of ×100 and ×10,000.”
Impact of level of nutritional dose and diet specific components of colostrum in promoting 24 h gain, circulating lipid profile, and circulating levels of immunocrit, proteins, glucose, and free amino acids in neonatal gilt piglets
Beckett, L. M., Ogundare, W., Reis, L. G., et al. (2026) · PLoS ONE · doi:10.1371/journal.pone.0341179 · Cited by 3
“Lipid extraction was performed according to the Bligh & Dyer protocol [23].”
Ultrasensitive Detection of Tissue Lipids at the Femtomole Scale Using Low-Microflow Targeted Lipidomics
Murtagh, O. L., Lowrey, P. E., & Hsu, K. (2026) · Analytical Chemistry · doi:10.1021/acs.analchem.5c05829 · Cited by 3
Recent Advancements in the Fisheries By-Products Processing Technologies as Sustainable Feedstuff for Aquaculture: Current Knowledge and Future Perspectives
Torsabo, D., Danba, E. P., Akange, E. T., Ariffin, H. K., Luthada-Raswiswi, R., Jimoh, J. O., Pius, A. F., Ishak, S. D., & Noordin, N. M. (2025) · Journal of Aquatic Food Product Technology · doi:10.1080/10498850.2025.2592663 · Cited by 3
“Microwave treatment has been employed for innovative microwave-assisted lipid extraction, serving as an alternative to conventional extraction methods such as Bligh and Dyer (1959), Soxhlet (1879), and Folch et al. ( .”
Valorization of agro-industrial residues for the cultivation of Pleurotus ostreatus var. florida: yield, nutritional quality, and physicochemical assessment
de Albuquerque, V. T. N., Fernandes, J. V. F., Grisi, C. V. B., et al. (2026) · Mycological Progress · doi:10.1007/s11557-026-02128-0 · Cited by 3
“The lipid concentration was obtained for Bligh and Dyer method (Bligh and Dyer 1959).”
Abiotic stress effects on polyunsaturated fatty acid biosynthesis in Chlorococcum oleofaciens and Leptolyngbya sp.
Chaudhary, N., Mishra, M., Yadav, A. K., et al. (2026) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-026-13754-9 · Cited by 3
“Microalgal lipid extraction followed Bligh and Dyer (1959) method.”
Restoring mitochondrial lipid homeostasis with arachidonic acid supplementation to alleviate cognitive impairment in schizophrenia patients
Gao, Y., Wang, D., Wang, Q., Wang, J., Li, S., Lei, F., Zhou, J., Hu, X., & Wan, C. (2026) · Journal of Advanced Research · doi:10.1016/j.jare.2026.03.047 · Cited by 3
Gummies as a Novel Approach to Valorize Rainbow Trout Wastes
Alak, G., OZAKAR, R. S., Özakar, E., et al. (2026) · Journal of Food Science · doi:10.1111/1750-3841.70992 · Cited by 3
“With reference to Kara et al. ( 2024 ) and Bligh and Dyer ( 1959 ), proximate analyses were performed in three parallel steps as follows.”
Seasonal effect on the biochemical composition of Atlantic seaweeds cultivated in integrated multitrophic aquaculture
Mendes, M., Conde, T., Moreira, A. S. P., Lopes, D., Pais, R., Batista, J., Neves, M., Salvador, M., Ferreira, A. S., Oliveira, K., et al. (2026) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2026.109098 · Cited by 3
A strategy to improve the annotation coverage of small molecule compounds in fruits, vegetables and their products by untargeted metabolomics
Yu, J., Xu, L., Zhang, N., et al. (2026) · Food Innovation and Advances · doi:10.48130/fia-0026-0009 · Cited by 3
Lysophospholipid signaling coordinates outer membrane homeostasis in Escherichia coli
Enverso, G., & Trent, M. S. (2026) · mBio · doi:10.1128/mbio.00567-26 · Cited by 3
Loss of proximal tubule lactate dehydrogenase A exacerbates nephrotoxic acute kidney injury through metabolic dysregulation
Lu, Y., Zmijewska, A. A., Zhang, Y., Osis, G., Cheung, M. D., Wilson, L. S., Jiang, Y., Vejendla, S., Traylor, A., Barnes, S., et al. (2026) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00048.2026 · Cited by 3
Indigenous microalgal biodiversity for efficient phycoremediation and biomass valorization of oil-drilling effluent
Muthukumaran, M. (2026) · Journal of Water Process Engineering · doi:10.1016/j.jwpe.2026.110190 · Cited by 3
Bacteroides-derived endocannabinoid-like commendamide attenuates skeletal muscle ferroptosis in vitro: implications for Duchenne muscular dystrophy
Muraglia, N. D., vito, M. D., Panza, E., Pagano, E., Vitale, R. M., Piscitelli, F., Villano, R., Capasso, R., Pota, V., Pace, C., et al. (2026) · Journal of Translational Medicine · doi:10.1186/s12967-026-08407-4 · Cited by 4
“Fecal samples collected from human individuals and mice were weighed in 1.5 ml Eppendorf tubes, subse‑ quently, the lipid fraction was extracted by following the procedure modified from [22].”
Influential
Possible therapeutic effects of myxobacterial metabolites on type I Gaucher disease
Kim, S., Kang, S., & Kim, J.-B. (2012) · Gene · doi:10.1016/j.gene.2012.05.036 · Cited by 3
Chemical composition of dried eyeballs from Euphausia superba and Euphausia pacifica
Yoshitomi, B., & YAMAGUCHI, H. (2007) · Fisheries Science · doi:10.1111/j.1444-2906.2007.01451.x · Cited by 3
Effects of Chain Length and Saturability of Fatty Acids on Phospholipids and Proteins in Plasma Membranes of Bovine Mammary Gland
Yan, Q., Tang, S., Han, X., et al. (2016) · The Journal of Membrane Biology · doi:10.1007/s00232-016-9919-9 · Cited by 3
Functional Characterization of Bacteriophage Resistant Mutants of Probiotic B. coagulans
Pandey, K., & Vakil, B. (2016) · Journal of Food Microbiology Safety & Hygiene · doi:10.4172/2476-2059.1000106 · Cited by 9
Monitoring Microalgal Neutral Lipid Accumulation with Nile Red
Natunen, K. (2017) · Methods in molecular biology · doi:10.1007/7651_2017_102 · Cited by 4
Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
Fazil, N. A., Alam, M. Z., Azmi, A. S., et al. (2018) · Malaysian Journal of Microbiology · doi:10.21161/mjm.39018 · Cited by 3
Growth of the longline-cultured sea squirt Halocynthia roretzi in a temperate bay of Korea: Biochemical composition and physiological energetics
Lee, Y. J., Wilberg, M. J., Han, E., et al. (2019) · Aquaculture · doi:10.1016/j.aquaculture.2019.734526 · Cited by 3
Recent Progress in Emerging Microalgae Technology for Biofuel Production
Jeslin, J., Koshy, A., Chamundeeswari, M., & Verma, M. L. (2020) · Clean Energy Production Technologies · doi:10.1007/978-981-32-9607-7_4 · Cited by 4
Intramuscular fatty acid composition of steers finished on oat pastures mixture with vetch or cornmeal supplementation
Taffarel, T. C., de Menezes, L. F. G., Kuss, F., et al. (2020) · Ciência Rural · doi:10.1590/0103-8478cr20200030 · Cited by 3
Hydroquinone exposure accumulates neutral lipid by the activation of CDP-DAG pathway in Saccharomyces cerevisiae
Raj, A., & Nachiappan, V. (2021) · Toxicology Research · doi:10.1093/toxres/tfab005 · Cited by 3
Lipophorin transport of hydrocarbon during early vitellogenesis in the silkworm, Bombyx mori
Pratheep, T., Krishnan, M., & Muthu, R. (2021) · Journal of Asia-Pacific Entomology · doi:10.1016/j.aspen.2021.02.010 · Cited by 3
Application of an association of yeast and lactic acid bacteria to bioencapsulate carotenoids in Daphnia magna (Straus, 1820)
Vasina, L., Kraievska, I., Khudyi, O., et al. (2020) · Fisheries & Aquatic Life · doi:10.2478/aopf-2020-0027 · Cited by 3
Impact of Culture Media Composition, Nutrients Stress and Gamma Radiation on Biomass and Lipid of the Green Microalga, Dictyochloropsis splendida as a Potential Feedstock for Biodiesel Production
Shanab, S. M. M., Abo-State, M. A. M., & Ali, H. E. A. (2021) · Baghdad Science Journal · doi:10.21123/bsj.2022.19.1.0043 · Cited by 3
The Use of Natural Fiber-Rich Food Product Is Safe and Reduces Aberrant Crypt Foci in a Pre-Clinical Model
do Amaral, L. A., de Almeida, T. D. S. F., de Souza, G. H. O., Baranoski, A., Maris, R. S., Bittencourt, F. F., Rafacho, B. P. M., Monreal, A. C. D., Kassuya, C. A. L., Antoniolli‐Silva, A. C. M. B., et al. (2021) · Nutrients · doi:10.3390/nu13082708 · Cited by 3
“Total lipids were determined by the method of Bligh & Dyer [32].”
Method
Acute response of refeeding in tambaqui submitted to long-term fasting
dos SANTOS, R. F., da SILVA, S. É. M., BRANDÃO, M. F., et al. (2021) · Revista Brasileira de Saúde e Produção Animal · doi:10.1590/s1519-99402122232021 · Cited by 3

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks