The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Abstract
Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“in addition to gravimetry measurements [14,15], a large panel of chromatography strategies have been developed, like thin-layer chromatography (TLC), high-pressure liquid chromatography (HPLC) and gas chromatography (GC) coupled with mass spectrometry (MS) [16].”
From The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae (2015) · cites this paper as [14]
Citations
Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citationBonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18
Research works printed but not linked: 1
- BANCROFT W., 1895, Phys. Iiev., 3, 1
Cited by
Sorted by citations
Items 44,501–44,600 of 48,847
Diversity analysis of papaya ('Carica papaya' L.) genotypes related to seed quality
Mengarda, L. H. G., Lopes, J. C., Zanotti, R. F., et al. (2015) · Australian Journal of Crop Science · Cited by 1
Zakeri, M., & Minabi, K. (2013) · Journal of the Persian Gulf (Marine Science) · Cited by 1
(2014) · Journal of Coastal Life Medicine · doi:10.12980/jclm.2.2014b1282 · Cited by 1
Amanlou, H., Maheri‐Sis, N., Bassiri, S., et al. (2012) · Cited by 1
Biofuel Production from Microalgae for Energy Applications
Basniwal, R. K., & Kaushik, P. (2014) · Cited by 1
Michel, D. G., Emmanuel, M. J., Excellent, M., et al. (2014) · Advance Journal of Food Science and Technology · doi:10.19026/ajfst.6.52 · Cited by 1
Development of Microbial Characterization Techniques for Low- Permeability Sedimentary Rocks
Slater, G. F., Moser, D. P., & Lollar, B. S. (2013) · Cited by 1
Palanisami, S., Viswanathan, T. V., Dudenhoeffer, N., Lee, K., & Nam, P. K.-S. (2013) · Cited by 1
Liu, Z. G., Wang, C. D., Hu, J. L., et al. (2015) · The Journal of Animal and Plant Sciences · Cited by 1
da Silva, T. L., & dos Reis, A. J. D. (2012) · Cellular origin and life in extreme habitats · doi:10.1007/978-94-007-5110-1_14 · Cited by 1
Tumor necrosis factor-alpha is a potential target for the neuroprotector Dimebon
Alessenko, A. V., Бачурин, С. О., Владимировна, Г. С., Karatasso, Y. O., Shevtsova, E. F., & Shingarova, L. N. (2015) · Biochemistry (Moscow) Supplement Series B Biomedical Chemistry · doi:10.1134/s199075081502002x · Cited by 1
Ghasemi, M., Atakishiyeva, Y., & Asadi, A. (2012) · Molecular biology research communications/Molecular biology research communications · doi:10.22099/mbrc.2012.240 · Cited by 1
Multivariate study of Nile tilapia byproducts enriched with omega-3 and dried with different methods
Zanqui, A. B., de Souza, A. H. P., Gohara, A. K., et al. (2016) · Food Science and Technology · doi:10.1590/1678-457x.6796 · Cited by 1
Growth and metabolic parameters of red tilapia reared in floating net cages in a small reservoir
Carneiro, P. C. F., Urbinati, E. C., & Roviero, D. P. (2000) · Cited by 1
The Effects of N(4-Methylphenyl)diphenimide on Lipid Metabolism of Sprague Dawley Rats
Hall, I. H., Wong, O. T., Simlot, R., et al. (1992) · Pharmaceutical Research · doi:10.1023/a:1015817720101 · Cited by 1
Sawyer, K. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/4k5k-t587 · Cited by 1
From strain to extraction - A review to produce biodiesel from microalgae in Bangladesh
Ishmam, S., Wasel-Ur-Rahman, Karim, S. B., Salehin, S., & Islam, A. K. M. S. (2016) · International Conference on the Developments in Renewable Energy Technology (ICDRET) · doi:10.1109/icdret.2016.7421484 · Cited by 1
EFFECT OF POST-HATCH FEED DEPRIVATION ON FATTY ACID COMPOSITION OF BROILER MEAT
Sugiharto, S., Isroli, I., Yudiarti, T., et al. (2015) · Journal of the Indonesian Tropical Animal Agriculture · doi:10.14710/jitaa.40.4.234-237 · Cited by 1
Itaya, Y., & Tokudome, Y. (2016) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.03.061 · Cited by 1
Characterization of flour obtained from waste of ca ssava minimally processed
Reis, I. A. O., de Souza, J. F., Rebouças, K. H., et al. (2011) · Cited by 1
de Crespi, M. P. L., Coll, J. F. C., Gomes, A. V. D. C., et al. (2008) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 1
Fish Oil Supplement Research Remains Murky
Moyer, M. W. (2012) · Nature · doi:10.1038/nature.2012.11484 · Cited by 1
Dias, V. A. D., Curi, R. A., Pereira, G. L., et al. (2016) · Pesquisa Agropecuária Brasileira · doi:10.1590/s0100-204x2016000200009 · Cited by 1
Physico-chemical and microbiological characteristics of dried Waragashi
le, P. T. F., Clement, A., Ayaba, N. A., et al. (2014) · African Journal of Food Science · doi:10.5897/ajfs2013.1103 · Cited by 1
Okamoto, M., & SAITO, H. (2011) · NIPPON SUISAN GAKKAISHI · doi:10.2331/suisan.77.1083 · Cited by 1
kandarajan, T. M., Eswar, A., Anbarasu, R., et al. (2014) · IOSR Journal of Environmental Science Toxicology and Food Technology · doi:10.9790/2402-08513240 · Cited by 1
Li, K. F., McNulty, B. A., Tierney, A. M., et al. (2013) · Proceedings of The Nutrition Society · doi:10.1017/s0029665113001766 · Cited by 1
Sugawara, S., Akahane, T., Fukui, K., & Tsukamoto, I. (2016) · Nippon Eiyo Shokuryo Gakkaishi · doi:10.4327/jsnfs.69.11 · Cited by 1
de Doctorado, T., & Tomac, A. (2013) · Cited by 1
Kumar, A., & Kumar, P. A. (2015) · Cited by 1
Changes in Composition of Vacuolar Membrane Lipid Fatty Acids Under Osmotic Stress
Озолина, Н. В., Нестеркина, И. С., Дударева, Л. В., et al. (2016) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 1
Huli, O., Suseno, S. H., & Santoso, J. (2014) · Cited by 1
Civera‐Cerecedo, R., Galicia, A., Nolasco‐Soria, H., et al. (2010) · Avances en Nutrición Acuicola · Cited by 1
Kt, A., Abdullaev, G. R., & Ulugbek, M. (2016) · American Journal of Biochemistry · Cited by 1
Abbes, I., Rihouey, C., Hardouin, J., et al. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2016.04.017 · Cited by 1
Wang, Y. (2016) · Science Discovery · doi:10.11648/j.sd.20160402.21 · Cited by 1
Nakamura, T., Hall, L., & Murphy, R. C. (1999) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-4793-8_79 · Cited by 1
Morishige, J., Tanaka, T., & Satouchi, K. (2012) · Methods in molecular biology · doi:10.1007/978-1-61779-800-9_4 · Cited by 1
The Change in Plasma PAF Activity before and after Delivery
Si, Y., Xia, H., Xiong, Z., et al. (1997) · Advances in experimental medicine and biology · doi:10.1007/978-1-4899-1813-0_83 · Cited by 1
Glasgow, W. C., & Eling, T. E. (1997) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-5325-0_68 · Cited by 1
Lipase and Phospholipase Inhibitors: Design and Applications
Venable, M. E. (2012) · Methods in molecular biology · doi:10.1007/978-1-61779-600-5_9 · Cited by 1
Alonso‐Fernández, A., & Saborido‐Rey, F. (2011) · Cited by 1
Introduction to tools and techniques for ceramide-centered research.
Kitatani, K., & Luberto, C. (2010) · PubMed · Cited by 1
Uemura, S., Sugiyama, K., Inoue, Y., Hamada, S., Katsuki, R., Maeda, Y., & Nakashima, M. (2012) · PubMed · Cited by 1
30. Total trans fatty acid in spreadable cheese
Barra, P. M. C., Barra, M. M., & de Oliveira, M. A. L. (2014) · Wageningen Academic Publishers eBooks · doi:10.3920/978-90-8686-766-0.030 · Cited by 1
Strain Selection for Biodiesel Production
Karthikeyan, S. (2013) · CRC Press eBooks · doi:10.1201/b14920-4 · Cited by 1
Practical identification of individual lipid species in lipid extracts of biological samples
Christie, W. W., & Han, X. (2012) · Elsevier eBooks · doi:10.1533/9780857097866.339 · Cited by 1
Komilus, C. F., Koshio, S., Ishikawa, M., et al. (2008) · Aquaculture Science (Japan) · doi:10.11233/aquaculturesci.56.303 · Cited by 1
Heterotrophic Algal-Biodiesel Production: Challenges and Opportunities
Cooney, M. J., & Guay, C. K. H. (2010) · American Society of Civil Engineers eBooks · doi:10.1061/9780784410899.ch15 · Cited by 1
Role of thiamine and its moieties in growth rate of diatom sp.
Jagannathan, N., & Prathap, P. (2011) · International Journal of Pharma and Bio Sciences · Cited by 1
Protocols on Lipid Extraction from Wet Algal Biomass
Naghdi, F. G., & Schenk, P. M. (2015) · Springer protocols handbooks/Springer protocols · doi:10.1007/8623_2015_131 · Cited by 1
Isolation and Analysis of Membrane Phospholipids as Biomarkers
Hachicho, N., & Heipieper, H. J. (2014) · Springer protocols handbooks/Springer protocols · doi:10.1007/8623_2014_17 · Cited by 1
Ayten, G., & Dikel, S. (2002) · Cited by 1
El-Gama, A. A. E., Fakhry, E. M., & Maghraby, D. M. E. (2015) · Journal of Coastal Life Medicine · doi:10.12980/jclm.3.2015j5-110 · Cited by 1
Hirayama, A., & Soga, T. (2016) · Wiley-VCH eBooks · doi:10.1002/9783527693801.ch10 · Cited by 1
Effects of Cooking on the Fatty Acid Content and Fatty Acid Composition of Pork
Sugiyama, S., & Ishinaga, M. (1999) · Journal of home economics · doi:10.11428/jhej1987.50.1119 · Cited by 1
Šafranko, Ž. M., Balog, T., Musa, M., Bujak, I. T., & Sobočanec, S. (2016) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-016-2802-2 · Cited by 1
Yokoyama, J. (2002) · Okayama Igakkai Zasshi (Journal of Okayama Medical Association) · doi:10.4044/joma1947.114.2_145 · Cited by 1
Utility of Corn Meal as Protein Source for Ayu Diet
Nakagawa, H., Furuhashi, M., Umino, T., et al. (2004) · Aquaculture Science · doi:10.11233/aquaculturesci1953.52.45 · Cited by 1
Icelandic blue mussels - A valuable high quality product
Desnica, N., Jensen, S., Þórarinsdóttir, G. G., et al. (2011) · Cited by 1
Obtention and Evaluation of Lyophilized Pulp Powder of Different Cultivars of Avocado
Kwiatkowski, Â., Oliveira, D. M., Marques, D. R., et al. (2014) · Zenodo (CERN European Organization for Nuclear Research) · doi:10.5281/zenodo.3348818 · Cited by 1
Borie, F., & Rubio, R. (2003) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 1
Chromatographic investigation of sugars and lipids from Tuber oligospermum and Terfezia arenaria
Harki, E. H., & Ghanmi, M. (2014) · Cited by 1
Borg, M. H., Svensson, M., Povlsen, J. V., Schmidt, E. B., Aalkjær, C., Christensen, J. H., & Ivarsen, P. (2016) · BMC Nephrology · doi:10.1186/s12882-016-0393-5 · Cited by 1
He, L., Lou, S., Lin, X., Qian, X., Xie, S., & Wang, Z. (2016) · Botanica Marina · doi:10.1515/bot-2016-0024 · Cited by 1
de Oliveira, E. A., Sampaio, A. A. M., Henrique, W., et al. (2015) · Boletim de Indústria Animal · doi:10.17523/bia.v72n3p241 · Cited by 1
Chaud, S. G., Sgarbieri, V. C., Vicente, E., da SILVA, N., Alves, A. B., Alvaro, J., & Mattos, R. D. (2007) · Cited by 1
Esther, M., Echevarría, R., Flores, G. C., et al. (2013) · Cited by 1
R&D on Microalgae for Sustainable Energy at TISTR
Mahakhant, A., Chansawang, N., Kunyalung, W., et al. (2010) · Cited by 1
Golvardzadeh, R., & Ardakani, S. A. Y. (2016) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 1
Munizzi, J. S. (2013) · Journal of International Crisis and Risk Communication Research · Cited by 1
Susilowati, R., Pratitis, A., & Januar, H. I. (2017) · SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology · doi:10.15578/squalen.v11i2.238 · Cited by 1
Fariña, A. C., Hirabara, S. M., Saín, J., González, M. A., Curi, R., & Bernal, C. (2017) · European Journal of Nutrition · doi:10.1007/s00394-017-1413-3 · Cited by 1
Membrane Lipids as Indicators for Viable Bacterial Communities Inhabiting Petroleum Systems
Gruner, A., Mangelsdorf, K., Vieth‐Hillebrand, A., Horsfield, B., van der Kraan, G. M., Köhler, T., Janka, C., Morris, B. E. L., & Wilkes, H. (2017) · Microbial Ecology · doi:10.1007/s00248-017-0954-6 · Cited by 1
Follicular fluid lipidomics reveals lipid alterations by LH addition during IVF cycles
da Costa, L. D. V. T., Cordeiro, F. B., Rochetti, R. C., Murgu, M., Zylbersztejn, D. S., Cedenho, A. P., Turco, E. G. L., & Fraietta, R. (2017) · Metabolomics · doi:10.1007/s11306-017-1207-x · Cited by 1
RESTRICTIONS TO USE OVEN IN DETERMINING WATER CONTENT FOR BRAZIL NUTS
Carneiro, J. D. S., Nogueira, R. M., Pires, E. M., et al. (2017) · Nativa · doi:10.31413/nativa.v5i1.4053 · Cited by 1
Park, S. Y. (2014) · OhioLink ETD Center (Ohio Library and Information Network) · Cited by 1
Oluba, O. M., & Josiah, S. J. (2014) · Current research. Cardiology · doi:10.4172/2368-0512.1000011 · Cited by 1
Caracterização físico-química de pães do tipo francês, bolacha e de cachorro quente
Martini, N. O., Escobar, T. D., & Kaminski, T. A. (2016) · REVISTA DO INSTITUTO ADOLFO LUTZ · doi:10.53393/rial.2016.v75.33528 · Cited by 1
Incorporation and Bioconversion of Omega-3 Fatty Acids for Obtention of Enriched Fish
Santos, O. O., Carbonera, F., Vágula, J. M., Maruyama, S. A., & Visentainer, J. V. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-811413-1.00011-5 · Cited by 1
Bioinformatics Pertinent to Lipid Analysis in Biological Samples
Ma, J., Arbelo, U., Guerra, Y., et al. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-6996-8_13 · Cited by 1
Ochrem, A., Zapletal, P., Żychlińska‐Buczek, J., et al. (2017) · Czech Journal of Food Sciences · doi:10.17221/314/2016-cjfs · Cited by 1
dos Santos, F. L., de Azeredo, V. B., Sergio, A., et al. (2007) · Cited by 1
Reddy, A. M., Elavarasan, K., Bhandary, M. H., et al. (2017) · International Journal of Current Microbiology and Applied Sciences · doi:10.20546/ijcmas.2017.608.344 · Cited by 1
Direct Methanolysis of Oleaginous Yeast Biomass (Pseudozyma parantarctica) to Microbial Biodiesel
Areesirisuk, A., Chiu, C.-H., Yen, T. B., et al. (2017) · Key engineering materials · doi:10.4028/www.scientific.net/kem.753.259 · Cited by 1
Yamauchi, N., & Tanoue, R. (2017) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2017.1373588 · Cited by 1
Characterization of a Urochloa humidicola methanol extract as a phytogenic additive for ruminants
de Freitas, R. S. X., Nepomuceno, D. D. D., Modesto, E. C., et al. (2017) · Bioscience Journal · doi:10.14393/bj-v33n6a2017-34688 · Cited by 1
Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification
Wychen, S. V., & Laurens, L. M. L. (2017) · Methods in molecular biology · doi:10.1007/7651_2017_107 · Cited by 3
Iman, R. H. S., Yuanita, I., Mutia, R., et al. (2012) · Institutional Repositories DataBase (IRDB) · Cited by 1
Yoon, H., In-Tae, H. A. M., Kim, J., et al. (2017) · Journal of Fisheries and Marine Sciences Education · doi:10.13000/jfmse.2017.29.6.1945 · Cited by 1
Dietary Sucrose and CD36 Receptor in Non-Alcoholic Fatty Liver Disease (NAFLD)
Alexander Aguilera, A. (2017) · Journal of Liver Research Disorders & Therapy · doi:10.15406/jlrdt.2017.03.00066 · Cited by 1
", E. D. H., Barca, S., do Con", E., et al. (2016) · Fishes in Mediterranean Environments · doi:10.29094/fishmed.2016.004 · Cited by 1
Upendar, G., Thakurta, S. G., Chakrabarty, J., et al. (2018) · International Conference on Green Energy and Applications (ICGEA) · doi:10.1109/icgea.2018.8356289 · Cited by 1
LIPID STRUCTURE OF LIVER MICROSOMAL MEMBRANES, LIMITATIONS OF A MODEL
STIER, A., Kühnle, W., & Rösen, R. (1977) · Elsevier eBooks · doi:10.1016/b978-0-08-021523-5.50007-9 · Cited by 1
Santhanam, P., Jeyaraj, N., Jothiraj, K., et al. (2018) · Springer eBooks · doi:10.1007/978-981-10-7953-5_11 · Cited by 1
Lake, Y., Brhane, Y., Nigatu, M., et al. (2018) · International Research Journal of Pharmacy · doi:10.7897/2230-8407.09696 · Cited by 1
Новгородцева, Т., Novgorodtseva, T. P., Ходосова, К. К., et al. (2018) · Bulletin physiology and pathology of respiration · doi:10.12737/article_5b975490da7d45.64596344 · Cited by 1
Soualem, Z., Brixi, N. K., Beghdad, C., & Belarbi, M. (2018) · Phytothérapie · doi:10.3166/phyto-2018-0013 · Cited by 1
Ouerghemmi, I., Farhat, M. B., Harbeoui, H., et al. (2018) · Herba Polonica · doi:10.2478/hepo-2018-0015 · Cited by 1
Metadata
- Authors
- Bligh, E. G., & Dyer, W. J.
- Venue
- Canadian Journal of Biochemistry and Physiology
- Publisher
- Canadian Science Publishing
- Published
- 1959-08-01
- Volume
- 37
- Issue
- 8
- Pages
- 911-917
- ISSN
- 0576-5544 (ISSN-L)
- OpenAlex
- W2152311269
- Cited by
- 54,751
- References
- 5
- Citations by year
- 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
- FWCI
- 8.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Canadian Journal of Biochemistry and Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
- Keywords
- lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
- MeSH terms
- Fishes, Lipids
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review