Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Özgür, M. E., Erdoğan, S., Aydemir, S., et al. (2022) · Pakistan Journal of Zoology · doi:10.17582/journal.pjz/20211112131153 · Cited by 1
Western-style diet does not negatively affect the healthy aging benefits of lifelong restrictive feeding
Reijne, A. C., Talarovičová, A., Coolen, A., Bruggink, J. E., Čiapaitė, J., Bleeker, A., Groen, A. K., Reijngoud, D., Bakker, B. M., & van Dijk, G. (2022) · Nutrition and Healthy Aging · doi:10.3233/nha-220163 · Cited by 2
“Lipids were extracted according to Bligh and Dyer [36].”
A novel deuterium‐based model for measurement of exogenous surfactant using deuterium‐depleted water
Simonato, M., Ricci, F., Catozzi, C., et al. (2022) · Pediatric Pulmonology · doi:10.1002/ppul.26104 · Cited by 1
Production and quality assessment of biodiesel obtained by thermal process from wet microalgae biomass of Choricystis minor var. minor
Menezes, R. S., Soares, A. T., Castro, J. C. T., Alves, M. I. R., Lopes, R. G., Cruz, Y. R., Monteiro, M., Carliz, R. G., Aranda, D. A. G., Dérner, R. B., et al. (2022) · Biofuels · doi:10.1080/17597269.2022.2106641 · Cited by 1
“method of Bligh and Dyer (1959) modified [27] and applied to wet microalgal biomass of Choricistis minor var.”
Method
Lipid biomarker profiling of adult Brugia malayi using mass spectrometry detection
Niyomploy, P., Mangmee, S., Tipthara, P., Saeung, A., Reamtong, O., & Sangvanich, P. (2022) · 3 Biotech · doi:10.1007/s13205-022-03296-y · Cited by 1
“Lipid composition was extracted from adult males and females of fresh B. malayi according to a modified method from Bligh-Dyer extraction (Bligh and Dyer 1959).”
MethodResultInfluential
Dry biomass of Aspergillus niger in commercial diets for juveniles of Nile tilapia (Oreochromis niloticus) in the intermediate growth phase (15 to 70g)
Picoli, F., Michielin, T., Sgnaulin, T., et al. (2022) · Research Society and Development · doi:10.33448/rsd-v11i3.26362 · Cited by 1
Production and use of wastewater-cultured Tetraselmis suecica for rearing Chanos chanos: an assessment on the advantages of chemical flocculate algal pastes on the growth, survival, and proximate composition of milkfish larvae
Kumar, S. D., Devi, K. N., Krishnaveni, N., Gowthami, A., Gunabal, S., Sathiyaraj, G., Sinduja, S., Sridhar, P., Santhanam, P., Perumal, P., et al. (2022) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-022-03227-0 · Cited by 1
“The fatty acid contents of alga were done by adopting the protocols of Bligh and Dyer [37] and AOAC [38].”
The role of oxidative stress in hemorrhagic stroke and restorative effects of Mexidol
Воронина, Т. А., Крайнева, В. А., Золотов, Н. Н., Олеговна, К. С., & Valdman, E. A. (2022) · S S Korsakov Journal of Neurology and Psychiatry · doi:10.17116/jnevro202212208265 · Cited by 1
A simple method for fish silage production: Using yoghurt as inoculum
Özkütük, A. S., & Özyurt, G. (2022) · Ege Journal of Fisheries and Aquatic Sciences · doi:10.12714/egejfas.39.3.11 · Cited by 1
Elucidating Hepatic Lipidosis in Stray Cats Through Serum Biochemistry, Liver Histopathology and Liver RNA Expression of PPAR-δ and PPAR-γ
Salleh, F. H., Goh, Y. M., Lau, S. F., Rani, P. A. M. A., Radzi, R., Mazlan, M., Alashraf, A. R., Goh, S. H., Rahman, S. A., Mohidin, T. B. M., et al. (2022) · Sains Malaysiana · doi:10.17576/jsm-2022-5107-01 · Cited by 1
“Malnutrition also affects creatinine production as a product of muscle catabolism (Delanaye et al. 2017) presenting drastic plasma creatinine decline in patients suffering anorexia nervosa (Boag et al. 1985).”
Background
Effect of colchicine on physiological and biochemical properties of Rhodococcus qingshengii
Markova, Y. A., Belovezhets, L. A., Nurminsky, V. N., et al. (2022) · Vavilov Journal of Genetics and Breeding · doi:10.18699/vjgb-22-69 · Cited by 1
Breeding stage and tissue isotopic consistency suggests colony-level flexibility in niche breadth of an Arctic marine bird
Parkinson, K. J. L., Hennin, H. L., Gilchrist, H. G., Hobson, K. A., Hussey, N. E., & Love, O. P. (2022) · Oecologia · doi:10.1007/s00442-022-05267-9 · Cited by 1
“Since plasma is often high in lipids we then lipid extracted all plasma samples using a 2:1 choloroform:methanol solution (Bligh and Dyer 1959).”
Method
BENZOATE GROUP ATTACHMENT TO TEMPO PROVIDES ENHANCED DISCRIMINATION OF LIPOSOMES FABRICATED USING HUMAN LUNG NORMAL AND CARCINOMA CELLS
Gasymov, O. K., Bakhishova, M., Gasanova, R., et al. (2022) · Russian Journal of Biological Physics and Chemisrty · doi:10.29039/rusjbpc.2022.0512 · Cited by 1
Lack of Low-affinity Phosphate Transporter Pho91 Alters Lipid Metabolism in Yeast Saccharomyces cerevisiae
Mani, S., & Nachiappan, V. (2022) · Indian Journal of Science and Technology · doi:10.17485/ijst/v15i43.1264 · Cited by 1
Development of New Processed Products from Egyptian Red Sea-Costal Spider Conch (Lambis lambis) and Their Biochemical and Microbiological Evaluation
Amin, H. F., Hamza, N. E., & Ahmed, O. M. (2022) · Journal of Aquatic Food Product Technology · doi:10.1080/10498850.2022.2157689 · Cited by 1
“The fat content was estimated according to Bligh and Dyer (1959) using chloroform: methanol (2:1 v/ v) solvent mixture.”
Method
THE INFLUENCE OF LIGHT INTENSITY ON GROWTH AND BIOCHEMICAL CONSTITUENTS OF SPIRUUNA
RafiquI, I. M., Hassan, A., Sulebele, G., et al. (2002) · Khulna University Studies · doi:10.53808/kus.2002.4.2.0332-l · Cited by 1
Major Phospholipids of Selected Dairy Products as Determined by the HPLC-UVvis and 31P-NMR Methods
Diamante, L. M. (2022) · Eurasian Journal of Biological and Chemical Sciences · doi:10.46239/ejbcs.1141216 · Cited by 1
Algal blooms -a source of oil for biodiesel
Meghana, S., & Murthy, S. M. (2022) · SAARC Journal of Agriculture · doi:10.3329/sja.v20i2.63587 · Cited by 1
“The dried algal biomass was powdered and total lipids were extracted from the powder using Bligh and Dyer (1959) method.”
Method
Response of the cellular components to environmental factors indicates limiting factors of Microcystis in Lake Taihu
Tian, Y., Deng, J., & Li, M. (2022) · Aquatic Ecology · doi:10.1007/s10452-022-09997-z · Cited by 1
“Microcystis fatty acid concentrations determination Extraction was carried out using the methanol-chloroform method (Bligh and Dyer 1959).”
Method
Biosynthesis and Transfer of α-Elostearic Acid In Vivo in Momordica charantia L. Developing Seeds and In Vitro in Microsomal Fractions of These Seeds
Banaś, A., Jasieniecka‐Gazarkiewicz, K., & Klińska, S. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010848 · Cited by 1

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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