Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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[NO TITLE AVAILABLE]
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Anziani, P., Becker, J., Mignon, C., Arnaud-Barbe, N., Courtois, V., Izac, M., Pizzato, R., Abi‐Ghanem, J., Tran, V.-D., Sarafian, M. H., et al. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1036386 · Cited by 1
“Lipids were extracted using Bligh and Dyer liquid extraction method (Bligh and Dyer, 2011; Sündermann et al., 2016).”
Method
Sorption of Cd(II), Cr(VI), Ni(II), and Pb(II) onto unmodified sludge from a pond treating woolen processing industry wastewater
Faleschini, M., Giarratano, E., & Gil, M. N. (2023) · Water Air & Soil Pollution · doi:10.1007/s11270-023-06166-2 · Cited by 1
“The following biomolecules concentrations were measured: proteins (Lowry et al., 1951), carbohydrates (Gerchakov & Hatcher, 1972), and lipids (Bligh & Dyer, 1959).”
Method
Seasonal dynamics of the total lipid content in the body of the anchovy Engraulis encrasicolus (Linnaeus, 1758) in 2017-2020
Voykina, A., Bugaev, L. A., Tsybul'skaya, M. A., et al. (2023) · E3S Web of Conferences · doi:10.1051/e3sconf/202337101054 · Cited by 1
Exogenous tetracosahexaenoic acid modifies the fatty acid composition of human primary T lymphocytes and Jurkat T cell leukemia cells contingent on cell type
Irvine, N. A., West, A. L., von Gerichten, J., Miles, E. A., Lillycrop, K. A., Calder, P. C., Fielding, B. A., & Burdge, G. C. (2023) · Lipids · doi:10.1002/lipd.12372
“Cell lipids were extracted with chloroform/methanol (2:1, vol/vol; Bligh & Dyer, 1959), dried, dissolved in toluene and converted to fatty acid methyl esters (FAMEs) by incubation with acidified methanol containing 2% (vol/vol) sulfuric acid at 50 (cid”
Influence of harvest method on proximate composition, texture profile and protein oxidation of beef from grain-fed Nellore cattle
Salim, A. P. A. D. A., Suman, S. P., Viana, F. M., et al. (2023) · Acta Scientiarum Animal Sciences · doi:10.4025/actascianimsci.v45i1.59110 · Cited by 1
Retinyl Ester Analysis by Orbitrap Mass Spectrometry
Jansen, J. W. A., Haaker, M. W., Zaal, E. A., & Helms, J. B. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3207-9_5 · Cited by 2
Cultivation of Chlorella vulgaris in aquaculture wastewater as alternative nutrient source and better treatment process
Usha, S. Z., Rahman, M. R., Sarker, J., Hasan, S. J., Sultana, R., Nayma, Z., MUKTA, F. A., & Khatoon, H. (2021) · doi:10.60015/bjvas/v09i1a6 · Cited by 1
“…Shidmadzu) at the wavelength of 750 nm. Based on Marsh and Weinstein (1966) the lipid analysis was performed with the sulphuric acid-charring method, following the carbonization method using tripalmitin as the standard after extracting lipids according to the method of Bligh and Dyer (1959).”
A Biochemical Approach for Assessment of the Diversity of Symbiotic Dinoflagellates
Zhukova, N. V. (2003) · Russian Journal of Marine Biology · doi:10.1023/a:1026365728891 · Cited by 1
Whole Algal Biomass In situ Transesterification to Fatty Acid Methyl Esters as Biofuel Feedstocks
Laurens, L. M. L. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-407909-0.00022-5 · Cited by 1
Optimisation of biogas production from a microbial perspective
Hobbs, P. J., Ward, A. J., Rao, R. S., et al. (2008) · Rothamsted Repository (Rothamsted Repository) · Cited by 1
Comparison of the Physicochemical Properties of Meat and Viscera of Dried Abalone (Haliotis discus hannai) Prepared using Different Drying Methods
박정욱, 이영재, 박인배, et al. (2009) · Korean Journal of Food Preservation · Cited by 1
Phospholipase D and Its Role in Mast Cells
Cissel, D. S., Fraundorfer, P. F., & Beaven, M. A. (1999) · Springer eBooks · doi:10.1007/978-1-4612-2154-8_16 · Cited by 1
Photobioreactor technologies for high-throughput microalgae cultivation
Ojo, E. (2015) · UCL Discovery (University College London) · Cited by 1
The ecology of contaminant exposure in Uca pugnax (Smith):
Haroski, D. M. (2008) · Rutgers University Community Repository (Rutgers University) · doi:10.7282/t3d50n9f · Cited by 1
Localization of an alkyl-acetyl-glycerol-CDP-choline : cholinephosphotransferase activity in submitochondrial fractions of Tetrahymena pyriformis
Tellis, C. C., Pantazi, D., Ioachim, E. E., et al. (2003) · European Journal of Cell Biology · doi:10.1078/0171-9335-00343 · Cited by 1
The Role of Platelet-Activating Factor (PAF) in the Development of Chronic Subdural Hematoma
Hirashima, Y., Kato, R., Ohmori, T., et al. (1993) · Springer eBooks · doi:10.1007/978-4-431-68231-8_10 · Cited by 1
Rapid determination of selected meat components using near infrared reflectance spectroscopy
Lee, G.-H. D. (1984) · doi:10.31274/rtd-180813-6845 · Cited by 1
Evidence for Catabolic Defect of Triglyceride in Experimental Nephrosis
Komuro, F., Hirano, T., Furukawa, S., et al. (1990) · The Journal of Japan Atherosclerosis Society · doi:10.5551/jat1973.18.2_217 · Cited by 1
Phosphorus cycling in organic systems
Parham, R. (2014) · University Library - University of Saskatchewan (University of Saskatchewan) · Cited by 1
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Molochkina, E. M., Ozerova, I. B., & Burlakova, E. B. (1998) · Advances in behavioral biology · doi:10.1007/978-1-4615-5337-3_26 · Cited by 1
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Cleary, J. S. (2006) · Summit (Simon Fraser University) · Cited by 1
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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