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Cited by 657

The DOI matches its Crossref record; 78 of the 78 references it cites were found in a registry or an index.

USE OF SURFACE PLASMON RESONANCE TO PROBE THE EQUILIBRIUM AND DYNAMIC ASPECTS OF INTERACTIONS BETWEEN BIOLOGICAL MACROMOLECULES

Schuck, Peter·1997·Annual Review of Biophysics and Biomolecular Structure

Abstract

▪ Abstract Surface plasmon resonance biosensors have become increasingly popular for the qualitative and quantitative characterization of the specific binding of a mobile reactant to a binding partner immobilized on the sensor surface. This article reviews the use of this new technique to measure the binding affinities and the kinetic constants of reversible interactions between biological macromolecules. Immobilization techniques, the most commonly employed experimental strategies, and various analytical approaches are summarized. In recent years, several sources of potential artifacts have been identified: immobilization of the binding partner, steric hindrance of binding to adjacent binding sites at the sensor surface, and finite rate of mass transport of the mobile reactant to the sensor surface. Described here is the influence of these artifacts on the measured binding kinetics and equilibria, together with suggested control experiments.

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Crossref · 10.1146/annurev.biophys.26.1.541

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Metadata

Authors
Schuck, P.
Venue
Annual Review of Biophysics and Biomolecular Structure
Published
1997-06
Volume
26
Issue
1
Pages
541-566
ISSN
  • 1056-8700 (ISSN-L)
  • 1545-4266
OpenAlex
W2145572269
Cited by
657
References
78
FWCI
11.92
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: USE OF SURFACE PLASMON RESONANCE TO PROBE THE EQUILIBRIUM AND DYNAMIC ASPECTS OF INTERACTIONS BETWEEN BIOLOGICAL MACROMOLECULES. https://app.cochat.ai/papers/10.1146/annurev.biophys.26.1.541
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Schuck, P. (1997). USE OF SURFACE PLASMON RESONANCE TO PROBE THE EQUILIBRIUM AND DYNAMIC ASPECTS OF INTERACTIONS BETWEEN BIOLOGICAL MACROMOLECULES. Annual Review of Biophysics and Biomolecular Structure. https://doi.org/10.1146/annurev.biophys.26.1.541

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