CoChat
Sign Up
Cited by 65

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Triglyceride-rich lipoprotein lipolysis products increase blood-brain barrier transfer coefficient and induce astrocyte lipid droplets and cell stress

Lee, Linda L., Aung, Hnin H., Wilson, Dennis W., Anderson, Steven E., Rutledge, John C., Rutkowsky, Jennifer M.·2017·American Journal of Physiology-Cell Physiology
DOI 10.1152/ajpcell.00120.2016
Open this paper in CoChat — free account

Abstract

Elevation of blood triglycerides, primarily as triglyceride-rich lipoproteins (TGRL), has been linked to cerebrovascular inflammation, vascular dementia, and Alzheimer’s disease (AD). Brain microvascular endothelial cells and astrocytes, two cell components of the neurovascular unit, participate in controlling blood-brain barrier (BBB) permeability and regulating neurovascular unit homeostasis. Our studies showed that infusion of high physiological concentrations of TGRL lipolysis products (TGRL + lipoprotein lipase) activate and injure brain endothelial cells and transiently increase the BBB transfer coefficient ( K i = permeability × surface area/volume) in vivo. However, little is known about how blood lipids affect astrocyte lipid accumulation and inflammation. To address this, we first demonstrated TGRL lipolysis products increased lipid droplet formation in cultured normal human astrocytes. We then evaluated the transcriptional pathways activated in astrocytes by TGRL lipolysis products and found upregulated stress and inflammatory-related genes including activating transcription factor 3 (ATF3), macrophage inflammatory protein-3α (MIP-3α), growth differentiation factor-15 (GDF15), and prostaglandin-endoperoxide synthase 2 (COX2). TGRL lipolysis products also activated the JNK/cJUN/ATF3 pathway, induced endoplasmic reticulum stress protein C/EBP homologous protein (CHOP), and the NF-κB pathway, while increasing secretion of MIP-3α, GDF15, and IL-8. Thus our results demonstrate TGRL lipolysis products increase the BBB transfer coefficient ( K i), induce astrocyte lipid droplet formation, activate cell stress pathways, and induce secretion of inflammatory cytokines. Our observations are consistent with evidence for lipid-induced neurovascular injury and inflammation, and we, therefore, speculate that lipid-induced astrocyte injury could play a role in cognitive decline.

Overview

Integrity signals

Registry

Crossref · 10.1152/ajpcell.00120.2016

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 69

  1. 10.1038/nrn1824 · DOI · Paper page
  2. 10.1159/000213842 · DOI
  3. 10.1016/S0197-4580(00)00124-X · DOI · Paper page
  4. 10.1194/jlr.M061853 · DOI
  5. 10.1186/1752-0509-8-80 · DOI
  6. 10.1016/S0022-2275(20)40600-5 · DOI
  7. 10.1083/jcb.40.3.648 · DOI
  8. 10.1016/S0960-9822(01)00257-3 · DOI
  9. 10.1194/jlr.M600214-JLR200 · DOI
  10. 10.1016/j.neuroscience.2013.08.044 · DOI
  11. 10.1016/j.physbeh.2012.05.015 · DOI
  12. 10.1210/en.2004-1520 · DOI · Paper page
  13. 10.4049/jimmunol.0903475 · DOI
  14. 10.1152/ajpheart.00686.2006 · DOI
  15. 10.1038/sj.jcbfm.9600189 · DOI
  16. 10.1002/mrm.10524 · DOI
  17. 10.1111/j.1600-0609.1991.tb00564.x · DOI
  18. 10.1111/acel.12116 · DOI
  19. 10.3233/JAD-2007-11109 · DOI · Paper page
  20. 10.1074/jbc.M203989200 · DOI
  21. 10.2174/156720511796391836 · DOI
  22. 10.1111/j.1471-4159.2012.07660.x · DOI
  23. 10.3390/ijerph6041335 · DOI
  24. Hai T, 1999, Gene Expr, 7, 321
  25. 10.1111/j.1460-9568.2010.07379.x · DOI
  26. 10.1002/pro.5560031213 · DOI
  27. 10.1128/MCB.24.13.5721-5732.2004 · DOI
  28. 10.1161/STROKEAHA.107.497719 · DOI
  29. 10.1046/j.1365-2249.2002.01865.x · DOI
  30. 10.1002/cbin.10294 · DOI
  31. 10.1038/nprot.2008.211 · DOI · Paper page
  32. 10.1111/j.1356-9597.2004.00707.x · DOI
  33. 10.1371/journal.pone.0033208 · DOI
  34. 10.2967/jnumed.115.158998 · DOI
  35. 10.1016/j.bcp.2015.08.086 · DOI
  36. 10.1038/emm.2006.40 · DOI · Paper page
  37. 10.1016/j.bbamcr.2014.04.004 · DOI
  38. Liu HT, 2013, Chin Med J (Engl), 126, 1350, 10.3760/cma.j.issn.0366-6999.20121475 · DOI
  39. 10.1006/meth.2001.1262 · DOI · Paper page
  40. 10.1371/journal.pone.0072796 · DOI
  41. 10.1016/0005-2736(85)90179-8 · DOI · Paper page
  42. 10.3233/JAD-2012-120541 · DOI · Paper page
  43. 10.1016/j.neurobiolaging.2014.03.038 · DOI
  44. 10.1016/j.bbadis.2015.12.016 · DOI · Paper page
  45. 10.1002/mrm.25985 · DOI
  46. 10.1007/s00330-015-3749-5 · DOI
  47. 10.1016/j.brainres.2013.05.047 · DOI
  48. 10.1186/1742-2094-5-31 · DOI
  49. 10.1006/nbdi.2000.0304 · DOI · Paper page
  50. 10.4161/21688370.2014.978720 · DOI
  51. 10.1620/tjem.149.449 · DOI
  52. 10.1177/0192623310385254 · DOI
  53. 10.1111/j.1471-4159.2005.03175.x · DOI
  54. 10.1038/srep20975 · DOI
  55. 10.1159/000343211 · DOI
  56. 10.1111/j.1471-4159.2008.05415.x · DOI · Paper page
  57. 10.1128/MCB.19.11.7589 · DOI
  58. 10.1111/j.1460-9568.2005.04389.x · DOI
  59. 10.1016/j.bbrc.2015.05.034 · DOI
  60. 10.1194/jlr.M700505-JLR200 · DOI · Paper page
  61. 10.1152/ajpheart.01366.2007 · DOI
  62. 10.1523/JNEUROSCI.18-02-00751.1998 · DOI
  63. 10.1186/s12974-016-0673-9 · DOI
  64. 10.1016/j.pharep.2014.07.009 · DOI
  65. 10.1039/c4cp03260h · DOI
  66. 10.1186/1742-2094-8-150 · DOI
  67. 10.1097/00006123-199704000-00027 · DOI
  68. 10.1111/j.1471-4159.1987.tb03431.x · DOI
  69. 10.1016/0006-8993(85)90963-1 · DOI

Cited by

Sorted by citations

Metadata

Authors
Lee, L. L., Aung, H. H., Wilson, D. W., Anderson, S. E., Rutledge, J. C., & Rutkowsky, J. M.
Affiliations
  • Department of Internal Medicine, University of California, Davis, California;
  • Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, California; and
  • Department of Physiology and Membrane Biology, University of California, Davis, California
Venue
American Journal of Physiology-Cell Physiology
Publisher
American Physiological Society
Published
2017-04-01
Volume
312
Issue
4
Pages
C500-C516
ISSN
  • 0363-6143
  • 1522-1563
DOI
10.1152/ajpcell.00120.2016
Cited by
65
References
3
Language
en
Funders
  • HHS | NIH | National Institute on Aging (U.S. National Institute on Aging) (AG039094, AG045541)
  • Nora Eccels Harrison Endowed Chair in Diabetes Research Fund
  • HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (U24-DK092993-05S1, U24-DK092993)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review