Ultrastructural Localization of Intraneuronal Aβ-Peptide in Alzheimer Disease Brains
Abstract
Autopsied brain tissue from Alzheimer's disease patients and old non-demented controls was studied after immunocytochemistry with the 4G8 monoclonal antibody that recognizes amyloid-beta peptides. Intraneuronal 4G8-positive reaction product was detected in all of the studied brains. The same brain regions in the Alzheimer's disease samples consistently showed both more immunopositive neurons and more stained reaction product per neuron than those from the non-demented brains. Ultrastructurally, the immunopositive reaction product accumulated in clusters of cytoplasmic elements that had a lipofuscin-like appearance, showed a fibrogranular content and were also closely apposed to lipid droplets located either on their periphery or within them. The most strongly 4G8-immunopositive elements had diffuse limits with their fibrogranular content free in the cytoplasm, whereas elements either without or showing only light immunoreaction had a limiting membrane. All immunopositive neurons displayed a similar pattern of clumping heterochromatin. The hypothetical neurotoxic role of intraneuronal amyloid-beta peptides free in the cytoplasm is discussed as is the possible relationship between the amyloid-beta peptides recognized by the 4G8 antibody and the lipid droplets which would presumably contain esterified cholesterol.
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Aβ staining could colocalize with or nonspecifically bind to lipofuscin.
“Aβ staining could colocalize with or nonspecifically bind to lipofuscin [33, 38]”
From Senescence-related impairment of autophagy induces toxic intraneuronal amyloid-β accumulation in a mouse model of amyloid pathology (2023) · cites this paper as [38]
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- Authors
- Gómez-Ramos, P., & Asunción Morán, M.
- Affiliations
- Department of Anatomy, Histology and Neuroscience, School of Medicine, Autónoma University of Madrid, Spain
- Venue
- Journal of Alzheimer's Disease
- Publisher
- SAGE Publications
- Published
- 2007-03-01
- Volume
- 11
- Issue
- 1
- Pages
- 53-59
- ISSN
- 1387-2877 (ISSN-L)
- 1875-8908
- OpenAlex
- W1597345104
- Cited by
- 76
- References
- 36
- Citations by year
- 2025: 3 · 2024: 7 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 7 · 2019: 2 · 2018: 3 · 2017: 6 · 2016: 7 · 2015: 4 · 2014: 3 · 2013: 1 · 2012: 6
- FWCI
- 3.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Alzheimer s Disease (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- intraneuronal amyloid-beta, Alzheimer's disease
- MeSH terms
- Aged, Aged, 80 and over, Alzheimer Disease, Brain, Inclusion Bodies, Cholesterol Esters, Cytoplasm, Female, Humans, Immunoenzyme Techniques, Lipofuscin, Male, Microscopy, Electron, Neurons, Reference Values, Ultrasonography, Amyloid beta-Peptides
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