Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol
Abstract
We evaluated the role of estradiol and progesterone in allergic lung inflammation. Rats were ovariectomized (Ovx) and, 7 days later, were sensitized with ovalbumin (OA) and challenged after 2 wk with inhaled OA; experiments were performed 1 day thereafter. Ovx-allergic rats showed reduced cell recruitment into the bronchoalveolar lavage (BAL) fluid relative to sham-Ovx allergic rats, as was observed in intact allergic rats treated with ICI-182,780. Estradiol increased the number of cells in the BAL of Ovx-allergic rats, whereas progesterone induced an additional reduction. Cells of BAL and bone marrow (BM) of Ovx-allergic rats released elevated amounts of IL-10 and reduced IL-1β and TNF-α. BM cells of Ovx-allergic rats released increased amounts of IL-10 and lower amounts of IL-4. Estradiol treatment of Ovx-allergic rats decreased the release of IL-10 but increased that of IL-4 by BM cells. Estradiol also caused an increased release of IL-1β and TNF-α by BAL cells. Progesterone significantly increased the release of IL-10, IL-1β, and TNF-α by BAL cells and augmented that of IL-4 by BM cells. Degranulation of bronchial mast cells from Ovx rats was reduced after in vitro challenge, an effect reverted by estradiol but not by progesterone. We suggest that the serum estradiol-to-progesterone ratio might drive cellular recruitment, modulating the pulmonary allergy and profile of release of anti-inflammatory or inflammatory cytokines. The existence of such dual hormonal effects suggests that the hormone therapy of asthmatic postmenopausal women and of those suffering of premenstrual asthma should take into account the possibility of worsening the pulmonary conditions.
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A relationship between female hormones, mast cell-derived mediators, and development of asthma and other allergic and inflammatory diseases has been suggested.
“In this regard, a relationship between female hormones, MC-derived mediators and development of asthma and other allergic and inflammatory diseases has been suggested [4, 16, 19].”
From Gender-Related Effects of Sex Steroids on Histamine Release and Fc<i>ε</i>RI Expression in Rat Peritoneal Mast Cells (2015) · cites this paper as [16]
Sex hormones have crucial effects on mast cell behavior in physiological and several pathological situations.
“Strong data in the last years reinforced the idea that sex hormones have crucial effects on MCs behavior, not only in physiological conditions, but also in several pathological situations [15–18].”
From Gender-Related Effects of Sex Steroids on Histamine Release and Fc<i>ε</i>RI Expression in Rat Peritoneal Mast Cells (2015) · cites this paper as [16]
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- Authors
- de Oliveira, A. P. L., Domingos, H. V., Cavriani, G., Damazo, A. S., dos Santos Franco, A. L., Oliani, S. M., Oliveira-Filho, R. M., Vargaftig, B. B., & de Lima, W. T.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2007-09
- Volume
- 293
- Issue
- 3
- Pages
- C1120-C1128
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2143526955
- Cited by
- 79
- References
- 54
- Citations by year
- 2025: 3 · 2024: 1 · 2022: 2 · 2020: 2 · 2019: 4 · 2018: 5 · 2017: 8 · 2016: 2 · 2015: 6 · 2014: 4 · 2013: 7 · 2012: 6
- FWCI
- 4.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion)
- UNESP Institutional Repository (São Paulo State University) (submittedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- allergic lung inflammation, progesterone, estradiol, estradiol/progesterone ratio, ovariectomy, ovalbumin sensitization, bronchoalveolar lavage, bone marrow cells, cytokine production, IL-10, IL-4, IL-1β, TNF-α, mast cell degranulation, cell recruitment, asthma, postmenopausal women, premenstrual asthma, fulvestrant
- MeSH terms
- Animals, Asthma, Bone Marrow Cells, Bronchoalveolar Lavage Fluid, Disease Models, Animal, Estradiol, Female, Hypersensitivity, Leukocyte Count, Mast Cells, Ovalbumin, Ovariectomy, Pneumonia, Progesterone, Tumor Necrosis Factor-alpha, Cell Degranulation, Interleukin-4, Cytokines, Interleukin-10, Rats, Wistar, Rats, Interleukin-1beta
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