Cited by 79Asthma and respiratory diseasesAllergic Rhinitis and SensitizationEstrogen and related hormone effects

Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol

de Oliveira, Ana Paula Ligeiro, Domingos, Helori Vanni, Cavriani, Gabriela, Damazo, Amilcar Sabino, dos Santos Franco, Adriana Lino, Oliani, Sonia Maria, Oliveira-Filho, Ricardo Martins, Vargaftig, Bernardo Boris +1 more·2007·American Journal of Physiology-Cell Physiology

Abstract

We evaluated the role of estradiol and progesterone in allergic lung inflammation. Rats were ovariectomized (Ovx) and, 7 days later, were sensitized with ovalbumin (OA) and challenged after 2 wk with inhaled OA; experiments were performed 1 day thereafter. Ovx-allergic rats showed reduced cell recruitment into the bronchoalveolar lavage (BAL) fluid relative to sham-Ovx allergic rats, as was observed in intact allergic rats treated with ICI-182,780. Estradiol increased the number of cells in the BAL of Ovx-allergic rats, whereas progesterone induced an additional reduction. Cells of BAL and bone marrow (BM) of Ovx-allergic rats released elevated amounts of IL-10 and reduced IL-1β and TNF-α. BM cells of Ovx-allergic rats released increased amounts of IL-10 and lower amounts of IL-4. Estradiol treatment of Ovx-allergic rats decreased the release of IL-10 but increased that of IL-4 by BM cells. Estradiol also caused an increased release of IL-1β and TNF-α by BAL cells. Progesterone significantly increased the release of IL-10, IL-1β, and TNF-α by BAL cells and augmented that of IL-4 by BM cells. Degranulation of bronchial mast cells from Ovx rats was reduced after in vitro challenge, an effect reverted by estradiol but not by progesterone. We suggest that the serum estradiol-to-progesterone ratio might drive cellular recruitment, modulating the pulmonary allergy and profile of release of anti-inflammatory or inflammatory cytokines. The existence of such dual hormonal effects suggests that the hormone therapy of asthmatic postmenopausal women and of those suffering of premenstrual asthma should take into account the possibility of worsening the pulmonary conditions.

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Crossref · 10.1152/ajpcell.00286.2006

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Authors
de Oliveira, A. P. L., Domingos, H. V., Cavriani, G., Damazo, A. S., dos Santos Franco, A. L., Oliani, S. M., Oliveira-Filho, R. M., Vargaftig, B. B., & de Lima, W. T.
Venue
American Journal of Physiology-Cell Physiology
Published
2007-09
Volume
293
Issue
3
Pages
C1120-C1128
ISSN
  • 0363-6143 (ISSN-L)
  • 1522-1563
OpenAlex
W2143526955
Cited by
79
References
54
Citations by year
2025: 3 · 2024: 1 · 2022: 2 · 2020: 2 · 2019: 4 · 2018: 5 · 2017: 8 · 2016: 2 · 2015: 6 · 2014: 4 · 2013: 7 · 2012: 6
FWCI
4.68
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Cell Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion)
  • UNESP Institutional Repository (São Paulo State University) (submittedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
  • Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
allergic lung inflammation, progesterone, estradiol, estradiol/progesterone ratio, ovariectomy, ovalbumin sensitization, bronchoalveolar lavage, bone marrow cells, cytokine production, IL-10, IL-4, IL-1β, TNF-α, mast cell degranulation, cell recruitment, asthma, postmenopausal women, premenstrual asthma, fulvestrant
MeSH terms
Animals, Asthma, Bone Marrow Cells, Bronchoalveolar Lavage Fluid, Disease Models, Animal, Estradiol, Female, Hypersensitivity, Leukocyte Count, Mast Cells, Ovalbumin, Ovariectomy, Pneumonia, Progesterone, Tumor Necrosis Factor-alpha, Cell Degranulation, Interleukin-4, Cytokines, Interleukin-10, Rats, Wistar, Rats, Interleukin-1beta

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