CoChat
Sign Up
Cited by 40Muscle metabolism and nutritionCardiovascular and exercise physiologyAdipose Tissue and Metabolism

Regulation of plasma long-chain fatty acid oxidation in relation to uptake in human skeletal muscle during exercise

Roepstorff, Carsten, Vistisen, Bodil, Roepstorff, Kirstine, Kiens, Bente·2004·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00001.2004
Open this paper in CoChat — free account

Abstract

In the present study, we investigated possible sites of regulation of long-chain fatty acid (LCFA) oxidation in contracting human skeletal muscle. Leg plasma LCFA kinetics were determined in eight healthy men during bicycling (60 min, 65% peak oxygen uptake) with either high (H-FOX) or low (L-FOX) leg fat oxidation (H-FOX: 1,098 ± 140; L-FOX: 494 ± 84 μmol FA/min, P < 0.001), which was achieved by manipulating preexercise muscle glycogen (H-FOX: 197 ± 21; L-FOX: 504 ± 25 mmol/kg dry wt, P < 0.001). Several blood metabolites and hormones were kept nearly similar between trials by allocating a preexercise meal and infusing glucose intravenously during exercise. During exercise, leg plasma LCFA fractional extraction was identical between trials (H-FOX: 17.8 ± 1.6; L-FOX: 18.2 ± 1.8%, not significant), suggesting similar LCFA transport capacity in muscle. On the contrary, leg plasma LCFA oxidation was 99% higher in H-FOX than in L-FOX (421 ± 47 vs. 212 ± 37 μmol/min, P < 0.001). Probably due to the slightly higher ( P < 0.01) plasma LCFA concentration in H-FOX than in L-FOX, leg plasma LCFA uptake was nonsignificantly ( P = 0.17) higher (25%) in H-FOX than in L-FOX, yet the fraction of plasma LCFA uptake oxidized was 61% higher ( P < 0.05) in H-FOX than in L-FOX. Accordingly, the muscle content of several lipid-binding proteins did not differ significantly between trials, although fatty acid translocase/CD36 and caveolin-1 were elevated ( P < 0.05) by the high-intensity exercise and dietary manipulation allocated on the day before the experimental trial. The present data suggest that, in contracting human skeletal muscle with different fat oxidation rates achieved by manipulating preexercise glycogen content, transsarcolemmal transport is not limiting plasma LCFA oxidation. Rather, the latter seems to be limited by intracellular regulatory mechanisms.

Integrity signals

Registry

Crossref · 10.1152/ajpendo.00001.2004

Cites

52 of 52 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • When fat oxidation is decreased and CD36 levels are increased, muscle uptake of long-chain fatty acids increases, resulting in increased muscle triglyceride synthesis and increased intramyocellular lipid content.

    “During conditions of decreased fat oxidation and increased CD36 levels, muscle uptake of LCFA increased, resulting in increased muscle triglyceride synthesis and increased IMCL content [97, 98].”

    From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [98]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Roepstorff, C., Vistisen, B., Roepstorff, K., & Kiens, B.
Affiliations
Copenhagen Muscle Research Centre (DK)
ORCID
Kiens, Bente
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2004-10
Volume
287
Issue
4
Pages
E696-E705
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00001.2004
OpenAlex
W2119950488
Cited by
40
References
52
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 2
FWCI
1.27
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
long-chain fatty acid oxidation, skeletal muscle, physical activity, muscle glycogen, fatty acid uptake, CD36, caveolin-1, high-intensity exercise
MeSH terms
Adult, Carrier Proteins, Diet, Eating, Fatty Acids, Glucose, Glycogen, Humans, Infusions, Intravenous, Leg, Male, Muscle Contraction, Oxidation-Reduction, Sarcolemma, Blotting, Western, Exercise, Respiratory Mechanics, Muscle, Skeletal, CD36 Antigens, Caveolins, Organic Anion Transporters, Fatty Acid-Binding Proteins, Caveolin 1, Caveolin 3

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review