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Cited by 243Muscle metabolism and nutritionFatty Acid Research and HealthAdipose Tissue and Metabolism

A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle

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Cameron-Smith, David, Burke, Louise M, Angus, Damien J, Tunstall, Rebecca J, Cox, Gregory R, Bonen, Arend, Hawley, John A, Hargreaves, Mark·2003·The American Journal of Clinical Nutrition
DOI 10.1093/ajcn/77.2.313
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Abstract

Background Dietary fatty acids may be important in regulating gene expression. However, little is known about the effect of changes in dietary fatty acids on gene regulation in human skeletal muscle.Objective The objective was to determine the effect of altered dietary fat intake on the expression of genes encoding proteins necessary for fatty acid transport and beta-oxidation in skeletal muscle.Design Fourteen well-trained male cyclists and triathletes with a mean (+/- SE) age of 26.9 +/- 1.7 y, weight of 73.7 +/- 1.7 kg, and peak oxygen uptake of 67.0 +/- 1.3 mL x kg(-1) x min(-1) consumed either a high-fat diet (HFat: > 65% of energy as lipids) or an isoenergetic high-carbohydrate diet (HCho: 70-75% of energy as carbohydrate) for 5 d in a crossover design. On day 1 (baseline) and again after 5 d of dietary intervention, resting muscle and blood samples were taken. Muscle samples were analyzed for gene expression [fatty acid translocase (FAT/CD36), plasma membrane fatty acid binding protein (FABPpm), carnitine palmitoyltransferase I (CPT I), beta-hydroxyacyl-CoA dehydrogenase (beta-HAD), and uncoupling protein 3 (UCP3)] and concentrations of the proteins FAT/CD36 and FABPpm.Results The gene expression of FAT/CD36 and beta -HAD and the gene abundance of FAT/CD36 were greater after the HFat than after the HCho diet (P < 0.05). Messenger RNA expression of FABPpm, CPT I, and UCP-3 did not change significantly with either diet.Conclusions A rapid and marked capacity for changes in dietary fatty acid availability to modulate the expression of mRNA-encoding proteins is necessary for fatty acid transport and oxidative metabolism. This finding is evidence of nutrient-gene interactions in human skeletal muscle.

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Crossref · 10.1093/ajcn/77.2.313

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  • CD36 has an important role in long-chain fatty acid uptake into skeletal muscle in rodents and humans, and its concentration and activity increase after exercise.

    “There is abundant evidence which indicates that CD36 plays an important role in LCFA uptake into skeletal muscle in rodents and human and its concentration and activity are increased following exercise [89–96].”

    From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [94]

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Authors
Cameron-Smith, D., Burke, L. M., Angus, D. J., Tunstall, R. J., Cox, G. R., Bonen, A., Hawley, J. A., & Hargreaves, M.
Affiliations
Deakin University (AU)
ORCID
Venue
The American Journal of Clinical Nutrition
Publisher
Elsevier BV
Published
2003-02
Volume
77
Issue
2
Pages
313-318
ISSN
0002-9165 (ISSN-L)
DOI
10.1093/ajcn/77.2.313
OpenAlex
W2125613801
Cited by
243
References
47
Citations by year
2026: 3 · 2025: 8 · 2024: 4 · 2023: 3 · 2022: 9 · 2021: 12 · 2020: 11 · 2019: 10 · 2018: 12 · 2017: 6 · 2016: 9 · 2015: 12 · 2014: 9 · 2013: 7 · 2012: 6
FWCI
6.32
Type
journal article
Language
en
Open access
Licence
Locations
  • American Journal of Clinical Nutrition (publishedVersion)
  • PubMed (publishedVersion)
  • RMIT Research Repository (RMIT University Library) (submittedVersion)
  • Deakin Research Online (Deakin University) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
high-fat diet, skeletal muscle, fatty acid uptake, FAT/CD36, carnitine palmitoyltransferase 1, beta-hydroxyacyl-CoA dehydrogenase, UCP3, fatty acid beta-oxidation, gene expression, dietary fatty acids, gene-nutrient interactions, lipid metabolism, trained cyclists
MeSH terms
Uncoupling Protein 3, Fatty Acid-Binding Protein 7, Adult, Bicycling, Carnitine O-Palmitoyltransferase, Carrier Proteins, Dietary Carbohydrates, Dietary Fats, Fatty Acids, Gene Expression Regulation, Humans, Ion Channels, Lipids, Male, Membrane Glycoproteins, Neoplasm Proteins, Oxidation-Reduction, RNA, Messenger, 3-Hydroxyacyl CoA Dehydrogenases, Exercise, Up-Regulation, Muscle, Skeletal, Cross-Over Studies, CD36 Antigens, Mitochondrial Proteins, Tumor Suppressor Proteins, Organic Anion Transporters, Lipid Metabolism, Fatty Acid-Binding Proteins

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