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Higher production of IL-8 in visceral vs. subcutaneous adipose tissue. Implication of nonadipose cells in adipose tissue
Abstract
IL-8 is released from human adipose tissue. Circulating IL-8 is increased in obese compared with lean subjects and is associated with measures of insulin resistance, development of atherosclerosis, and cardiovascular disease. We studied 1) the production and release of IL-8 in vitro from paired samples of subcutaneous (SAT) and visceral (VAT) adipose tissue and 2) the production of IL-8 from whole adipose tissue, isolated adipocytes, and nonfat cells of adipose tissue. IL-8 release from VAT was fourfold higher than from SAT ( P < 0.05), and IL-8 mRNA was twofold higher in VAT compared with SAT ( P < 0.01). Dexamethasone (50 nM) attenuated IL-8 production by 50% ( P < 0.05), and IL-1β (2 μg/l) increased IL-8 production up to 15-fold ( P < 0.001). IL-8 release from whole SAT explants correlated with body mass index (BMI; r = 0.78; P < 0.001), as did IL-8 release from nonfat cells ( r = 0.79; P < 0.001). However, no correlation was found between IL-8 release from the fraction of isolated adipocytes and BMI ( r = 0.01). In conclusion, we demonstrated an increased release of IL-8 from VAT compared with SAT. Furthermore, our data suggest that the observed elevation in circulating levels of IL-8 in obese subjects is due primarily to the release of IL-8 from nonfat cells from adipose tissue. The high levels of IL-8 release from human adipose tissue and accumulation of this tissue in obese subjects may account for some of the increase in circulating IL-8 observed in obesity.
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“L. salivarius UCC118 (10 10 CFU/d for 21 d) did not affect serum IL-1a, IL-1b, IL-4, sIL-2R, sIL-6R, TNF-a or IFN-g concentrations in healthy adults aged 20 -65 years (829) .”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [829]
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Metadata
- Authors
- Bruun, J. M., Lihn, A. S., Madan, A. K., Pedersen, S. B., Schiøtt, K. M., Fain, J. N., & Richelsen, B.
- Affiliations
- Aarhus University (DK)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2004-01
- Volume
- 286
- Issue
- 1
- Pages
- E8-E13
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2125302795
- Cited by
- 215
- References
- 31
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 9 · 2023: 7 · 2022: 3 · 2021: 7 · 2020: 9 · 2019: 8 · 2018: 7 · 2017: 12 · 2016: 6 · 2015: 7 · 2014: 11 · 2013: 12 · 2012: 14
- FWCI
- 9.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Sundhed og Sygdom, Det Frie Forskningsråd
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Keywords
- interleukin-8, visceral adipose tissue, subcutaneous adipose tissue, obesity, adipocytes, IL-8 mRNA expression, dexamethasone, IL-1β, body mass index, insulin resistance
- MeSH terms
- Adipose Tissue, Adult, Aerosols, Cell Differentiation, Cells, Cultured, Connective Tissue, Female, Fusarium, Humans, Middle Aged, Obesity, Morbid, RNA, Messenger, Stem Cells, Viscera, Body Mass Index, Interleukin-8, Stromal Cells, Adipocytes, Subcutaneous Tissue, Depsipeptides, In Vitro Techniques
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