Bone as an ion exchange system: evidence for a link between mechanotransduction and metabolic needs
Open accessAbstract
To detect whether the mutual interaction occurring between the osteocytes-bone lining cells system (OBLCS) and the bone extracellular fluid (BECF) is affected by load through a modification of the BECF-extracellular fluid (ECF; systemic extracellular fluid) gradient, mice metatarsal bones immersed in ECF were subjected ex vivo to a 2-min cyclic axial load of different amplitudes and frequencies. The electric (ionic) currents at the bone surface were measured by a vibrating probe after having exposed BECF to ECF through a transcortical hole. The application of different loads and different frequencies increased the ionic current in a dose-dependent manner. The postload current density subsequently decayed following an exponential pattern. Postload increment's amplitude and decay were dependent on bone viability. Dummy and static loads did not induce current density modifications. Because BECF is perturbed by loading, it is conceivable that OBLCS tends to restore BECF preload conditions by controlling ion fluxes at the bone-plasma interface to fulfill metabolic needs. Because the electric current reflects the integrated activity of OBLCS, its evaluation in transgenic mice engineered to possess genetic lesions in channels or matrix constituents could be helpful in the characterization of the mechanical and metabolic functions of bone.
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- Authors
- Rubinacci, A., Covini, M., Bisogni, C., Villa, I., Galli, M., Palumbo, C., Ferretti, M., Muglia, M. A., & Marotti, G.
- Affiliations
- IRCCS Ospedale San Raffaele (IT)
- Politecnico di Milano (IT)
- University of Modena and Reggio Emilia (IT)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2002-04-01
- Volume
- 282
- Issue
- 4
- Pages
- E851-E864
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2135293844
- Cited by
- 32
- References
- 42
- Citations by year
- 2026: 2 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- IRIS UNIMORE (University of Modena and Reggio Emilia) (submittedVersion, cc-by)
- Virtual Community of Pathological Anatomy (University of Castilla La Mancha) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- mechanotransduction, osteocytes, bone lining cells, extracellular fluid, ionic currents, bone viability, bone metabolism, metatarsals, transgenic mice
- MeSH terms
- Animals, Biomechanical Phenomena, Bone and Bones, Electric Conductivity, Energy Transfer, Extracellular Space, Mathematics, Mice, Transgenic, Microscopy, Electron, Microscopy, Electron, Scanning, Osteocytes, Weaning, Signal Transduction, Mice
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