Effect of weight loss on whole body and cellular lipid metabolism in severely obese humans
Abstract
We evaluated the effect of diet-induced weight loss on whole body and cellular lipid metabolism in persons with severe upper body obesity in two study protocols. In protocol 1, palmitate and glycerol rates of appearance (Ra) in plasma were determined during basal conditions in seven subjects [initial body mass index (BMI) = 41.3 +/- 2.2 kg/m2] before and after 20.4 +/- 3.0 kg weight loss. Total glycerol and palmitate Ra decreased from 231.0 +/- 19.4 and 166.2 +/- 16.6 mumol/min, respectively, before weight loss to 162.7 +/- 9.5 and 105.0 +/- 9.7 mumol/min, respectively, after weight loss (P < 0.01). However, glycerol and palmitate Ra expressed per kilogram fat mass were similar both before and after weight loss. In protocol 2, subcutaneous abdominal adipose tissue was obtained before and after 14.4 +/- 2.1 kg weight loss in five subjects (initial BMI = 41.6 +/- 2.6 kg/m2). Weight loss caused a 38 +/- 8% decrease in adipocyte hormone-sensitive lipase concentration (P < 0.05) but was not associated with any consistent changes in the concentrations of GTP-dependent regulatory proteins, Gi1 alpha, Gi2 alpha, and G3 alpha. We conclude that diet-induced weight loss ameliorates the increase in basal lipolytic rates in persons with severe upper body obesity. These alterations are associated with changes in cellular hormone-sensitive lipase but not GTP-dependent regulatory protein concentrations.
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Weight loss decreases the rate of release of fatty acids from adipose tissue.
“Weight loss decreases visceral fat mass, 26 intramyocellular fat, 27 intrahepatic fat, 28 fat-cell size, 29 and the rate of release of fatty acids from adipose tissue.30”
From Absence of an Effect of Liposuction on Insulin Action and Risk Factors for Coronary Heart Disease (2004) · cites this paper as [30]
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- Authors
- Klein, S. B., Luu, K., Gasic, S., & Green, A.
- Affiliations
- Washington University in St. Louis (US)
- ORCID
- Samuel B. Klein
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 1996-05-01
- Volume
- 270
- Issue
- 5
- Pages
- E739-E745
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W117206723
- Cited by
- 41
- References
- 0
- Citations by year
- 2018: 3 · 2015: 1 · 2014: 6 · 2013: 2 · 2012: 2
- FWCI
- 0.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dk049989-04)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-49989)
- National Center for Research Resources (RR.00036)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- diet-induced weight loss, morbid obesity, upper body obesity, adipose tissue lipolysis, hormone-sensitive lipase, subcutaneous abdominal fat, fat mass
- MeSH terms
- Adult, Sterol Esterase, Cytoplasm, Female, Hormones, Humans, Kinetics, L-Lactate Dehydrogenase, Male, Obesity, Morbid, Weight Loss, GTP-Binding Proteins, Lipid Metabolism
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