The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
AMPK signaling in contracting human skeletal muscle: acetyl-CoA carboxylase and NO synthase phosphorylation
Open accessAbstract
AMP-activated protein kinase (AMPK) is a metabolic stress-sensing protein kinase responsible for coordinating metabolism and energy demand. In rodents, exercise accelerates fatty acid metabolism, enhances glucose uptake, and stimulates nitric oxide (NO) production in skeletal muscle. AMPK phosphorylates and inhibits acetyl-coenzyme A (CoA) carboxylase (ACC) and enhances GLUT-4 translocation. It has been reported that human skeletal muscle malonyl-CoA levels do not change in response to exercise, suggesting that other mechanisms besides inhibition of ACC may be operating to accelerate fatty acid oxidation. Here, we show that a 30-s bicycle sprint exercise increases the activity of the human skeletal muscle AMPK-α1 and -α2 isoforms approximately two- to threefold and the phosphorylation of ACC at Ser 79(AMPK phosphorylation site) ∼8.5-fold. Under these conditions, there is also an ∼5.5-fold increase in phosphorylation of neuronal NO synthase-μ (nNOSμ) at Ser 1451. These observations support the concept that inhibition of ACC is an important component in stimulating fatty acid oxidation in response to exercise and that there is coordinated regulation of nNOSμ to protect the muscle from ischemia/metabolic stress.
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Cites
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Cited by
Physiological adaptations to low-volume, high-intensity interval training in health and disease
Gibala, M. J., Little, J. P., MacDonald, M. J., & Hawley, J. A. (2012) · The Journal of Physiology · doi:10.1113/jphysiol.2011.224725 · Cited by 1,744
Steinberg, G. R., & Kemp, B. E. (2009) · Physiological Reviews · doi:10.1152/physrev.00011.2008 · Cited by 1,637
Self-citationExercise, GLUT4, and skeletal muscle glucose uptake
Richter, E. A., & Hargreaves, M. (2013) · Physiological Reviews · doi:10.1152/physrev.00038.2012 · Cited by 1,407
Skeletal muscle energy metabolism during exercise
Hargreaves, M., & Spriet, L. L. (2020) · Nature Metabolism · doi:10.1038/s42255-020-0251-4 · Cited by 1,408
Carey, A. L., Steinberg, G. R., Macaulay, S. L., Thomas, W. G., Holmes, A. G., Ramm, G., Prelovsek, O., Hohnen-Behrens, C., Watt, M. J., James, D. E., et al. (2006) · Diabetes · doi:10.2337/db05-1404 · Cited by 898
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Metadata
- Authors
- Chen, Z.-P., McConell, G. K., Michell, B. J., Snow, R. J., Canny, B. J., & Kemp, B. E.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2000-11-01
- Volume
- 279
- Issue
- 5
- Pages
- E1202-E1206
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W1820085648
- Cited by
- 342
- References
- 31
- FWCI
- 5.07
- Type
- journal article
- Language
- en
- Open access
- green
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