The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
A guide to Ussing chamber studies of mouse intestine
Open accessAbstract
The Ussing chamber provides a physiological system to measure the transport of ions, nutrients, and drugs across various epithelial tissues. One of the most studied epithelia is the intestine, which has provided several landmark discoveries regarding the mechanisms of ion transport processes. Adaptation of this method to mouse intestine adds the dimension of investigating genetic loss or gain of function as a means to identify proteins or processes affecting transepithelial transport. In this review, the principles underlying the use of Ussing chambers are outlined including limitations and advantages of the technique. With an emphasis on mouse intestinal preparations, the review covers chamber design, commercial equipment sources, tissue preparation, step-by-step instruction for operation, troubleshooting, and examples of interpretation difficulties. Specialized uses of the Ussing chamber such as the pH stat technique to measure transepithelial bicarbonate secretion and isotopic flux methods to measure net secretion or absorption of substrates are discussed in detail, and examples are given for the adaptation of Ussing chamber principles to other measurement systems. The purpose of the review is to provide a practical guide for investigators who are new to the Ussing chamber method.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Ionic channels of excitable membranes
(1985) · General Pharmacology: The Vascular System · doi:10.1016/0306-3623(85)90172-7 · Cited by 6,982
Physiology of the Gastrointestinal Tract
(2006) · Elsevier eBooks · doi:10.1016/b978-0-12-088394-3.x5000-4 · Cited by 4,597
Webb, A. K. (2006) · Cited by 4,369
[6] Correction for liquid junction potentials in patch clamp experiments
Neher, E. (1992) · Methods in Enzymology · doi:10.1016/0076-6879(92)07008-c · Cited by 1,132
An Animal Model for Cystic Fibrosis Made by Gene Targeting
Snouwaert, J. N., Brigman, K. K., Latour, A. M., Malouf, N. N., Boucher, R. C., Smithies, O., & Koller, B. H. (1992) · Science · doi:10.1126/science.257.5073.1083 · Cited by 918
Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger
Schultheis, P. J., Clarke, L. L., Meneton, P., Miller, M. L., Soleimani, M., Gawenis, L. R., Riddle, T. M., Duffy, J. J., Doetschman, T., Wang, T., et al. (1998) · Nature Genetics · doi:10.1038/969 · Cited by 869
Self-citationActive sugar transport in health and disease
Wright, E. M., Hirayama, B. A., & Loo, D. F. (2007) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.2006.01746.x · Cited by 627
Ion Transport in Isolated Rabbit Ileum
Schultz, S. G., & Zalusky, R. (1964) · The Journal of General Physiology · doi:10.1085/jgp.47.3.567 · Cited by 558
Ion Transport in Isolated Rabbit Ileum
Schultz, S. G., & Zalusky, R. (1964) · The Journal of General Physiology · doi:10.1085/jgp.47.6.1043 · Cited by 513
Ionic Conductances of Extracellular Shunt Pathway in Rabbit Ileum
Frizzell, R. A., & Schultz, S. G. (1972) · The Journal of General Physiology · doi:10.1085/jgp.59.3.318 · Cited by 415
Cited by
Intestinal permeability – a new target for disease prevention and therapy
Bischoff, S. C., Barbara, G., Buurman, W., Ockhuizen, T., Schulzke, J.-D., Serino, M., Tilg, H., Watson, A., & Wells, J. M. (2014) · BMC Gastroenterology · doi:10.1186/s12876-014-0189-7 · Cited by 1,755
The intestinal barrier: a fundamental role in health and disease
Vancamelbeke, M., & Vermeire, S. (2017) · Expert Review of Gastroenterology & Hepatology · doi:10.1080/17474124.2017.1343143 · Cited by 1,287
Carbonell‐Capella, J. M., Buniowska, M., Barba, F. J., Esteve, M. J., & Frígola, A. (2014) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12049 · Cited by 656
Orally Absorbed Cyclic Peptides
Nielsen, D. S., Shepherd, N. E., Xu, W., Lucke, A. J., Stoermer, M. J., & Fairlie, D. P. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00838 · Cited by 445
Boyd, B. J., Bergström, C. A. S., Vinarov, Z., Kuentz, M., Brouwers, J., Augustijns, P., Brandl, M., Bernkop-Schnürch, A., Shrestha, N., Préat, V., et al. (2019) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2019.104967 · Cited by 367
Metadata
- Authors
- Clarke, L. L.
- Venue
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Publisher
- American Physiological Society
- Published
- 2009-06
- Volume
- 296
- Issue
- 6
- Pages
- G1151-G1166
- ISSN
- 0193-1857 (ISSN-L)
- 1522-1547
- OpenAlex
- W2157757382
- Cited by
- 419
- References
- 48
- FWCI
- 3.72
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A guide to Ussing chamber studies of mouse intestine. https://app.cochat.ai/papers/10.1152/ajpgi.90649.2008
- The paper
- Clarke, L. L. (2009). A guide to Ussing chamber studies of mouse intestine. American Journal of Physiology-Gastrointestinal and Liver Physiology. https://doi.org/10.1152/ajpgi.90649.2008
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
