Cited by 94Birth, Development, and HealthCardiovascular Function and Risk FactorsGestational Diabetes Research and Management

Maternal high-fat diet impairs cardiac function in offspring of diabetic pregnancy through metabolic stress and mitochondrial dysfunction

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Mdaki, Kennedy S., Larsen, Tricia D., Wachal, Angela L., Schimelpfenig, Michelle D., Weaver, Lucinda J., Dooyema, Samuel D. R., Louwagie, Eli J., Baack, Michelle L.·2016·American Journal of Physiology-Heart and Circulatory Physiology

Abstract

Offspring of diabetic pregnancies are at risk of cardiovascular disease at birth and throughout life, purportedly through fuel-mediated influences on the developing heart. Preventative measures focus on glycemic control, but the contribution of additional offenders, including lipids, is not understood. Cellular bioenergetics can be influenced by both diabetes and hyperlipidemia and play a pivotal role in the pathophysiology of adult cardiovascular disease. This study investigated whether a maternal high-fat diet, independently or additively with diabetes, could impair fuel metabolism, mitochondrial function, and cardiac physiology in the developing offspring's heart. Sprague-Dawley rats fed a control or high-fat diet were administered placebo or streptozotocin to induce diabetes during pregnancy and then delivered offspring from four groups: control, diabetes exposed, diet exposed, and combination exposed. Cardiac function, cellular bioenergetics (mitochondrial stress test, glycolytic stress test, and palmitate oxidation assay), lipid peroxidation, mitochondrial histology, and copy number were determined. Diabetes-exposed offspring had impaired glycolytic and respiratory capacity and a reduced proton leak. High-fat diet-exposed offspring had increased mitochondrial copy number, increased lipid peroxidation, and evidence of mitochondrial dysfunction. Combination-exposed pups were most severely affected and demonstrated cardiac lipid droplet accumulation and diastolic/systolic cardiac dysfunction that mimics that of adult diabetic cardiomyopathy. This study is the first to demonstrate that a maternal high-fat diet impairs cardiac function in offspring of diabetic pregnancies through metabolic stress and serves as a critical step in understanding the role of cellular bioenergetics in developmentally programmed cardiac disease.

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Crossref · 10.1152/ajpheart.00795.2015

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Authors
Mdaki, K. S., Larsen, T. D., Wachal, A. L., Schimelpfenig, M. D., Weaver, L. J., Dooyema, S. D. R., Louwagie, E. J., & Baack, M. L.
Affiliations
  • Sanford Research (US)
  • University of South Dakota (US)
  • Augustana University (US)
Corresponding author
Baack, Michelle L.
Venue
American Journal of Physiology-Heart and Circulatory Physiology
Published
2016-03-15
Volume
310
Issue
6
Pages
H681-H692
ISSN
  • 0363-6135 (ISSN-L)
  • 1522-1539
OpenAlex
W2299303320
Cited by
94
References
64
Citations by year
2026: 3 · 2025: 6 · 2024: 12 · 2023: 12 · 2022: 17 · 2021: 14 · 2020: 7 · 2019: 4 · 2018: 9 · 2017: 7 · 2016: 3
FWCI
8.92
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Heart and Circulatory Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • NIH-NICHD (K08HD078504, R25HD072596)
  • NIH-NIGMS (P20GM103548, P20GM103620-01A1)
  • NSF-REU (DBI-1262744)
  • NSF-EPSCOR (1355423)
  • Sanford School of Medicine - University of South Dakota (Faculty Grant)
  • Sanford - South Dakota State University Research Collaborative Grant
Topics
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
  • Health Sciences › Medicine › Obstetrics and Gynecology › Gestational Diabetes Research and Management
Keywords
maternal high-fat diet, diabetes in pregnancy, mitochondrial dysfunction, metabolic stress, cellular bioenergetics, glycolytic capacity, mitochondrial DNA copy number, lipid peroxidation, palmitate oxidation, diastolic dysfunction, diabetic cardiomyopathy, streptozotocin-induced diabetes, hyperlipidemia
MeSH terms
Animals, Animals, Newborn, Diabetes Mellitus, Experimental, DNA, Mitochondrial, Echocardiography, Female, Glycolysis, Heart, Mitochondria, Heart, Pregnancy, Stress, Physiological, Lipid Peroxidation, Diabetes, Gestational, Rats, Sprague-Dawley, Cell Respiration, Myocytes, Cardiac, Rats, Diet, High-Fat

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