Cited by 97Muscle Physiology and DisordersHormonal and reproductive studiesEstrogen and related hormone effects

Sexually dimorphic expression of myosin heavy chains in the adult mouse masseter

Eason, Jane M., Schwartz, Gail A., Pavlath, Grace K., English, Arthur W.·2000·Journal of Applied Physiology

Abstract

Little is known regarding the role of androgenic hormones in the maintenance of myosin heavy chain (MHC) composition of rodent masticatory muscles. Because the masseter is the principal jaw closer in rodents, we felt it was important to characterize the influence of androgenic hormones on the MHC composition of the masseter. To determine the extent of sexual dimorphism in the phenotype of masseter muscle fibers of adult (10-mo-old) C57 mice, we stained tissue sections with antibodies specific to type IIa and IIb MHC isoforms. Females contain twice as many fibers containing the IIa MHC as males, and males contain twice as many fibers containing the IIb MHC as females. There is a modest amount of regionalization of MHC phenotypes in the mouse masseter. The rostral portions of the masseter are composed mostly of type IIa fibers, whereas the midsuperficial and caudal regions contain mostly type IIb fibers. Using immunoblots, we showed that castration results in an increase in the expression of type IIa MHC fibers in males. Ovariectomy has no effect on the fiber type composition in females. We conclude that testosterone plays a role in the maintenance of MHC expression in the adult male mouse masseter.

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Crossref · 10.1152/jappl.2000.89.1.251

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  • The mouse masseter is composed of high numbers of IIX fibers in both sexes; however, there are threefold fewer IIB fibers and more IIA fibers in females compared with males.

    “The mouse masseter is composed of high numbers of IIX fibers in both sexes; however, there are threefold fewer IIB fibers and more IIA fibers in females compared with males (27).”

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Authors
Eason, J. M., Schwartz, G. A., Pavlath, G. K., & English, A. W.
Affiliations
  • Emory University (US)
  • Departments of Cell Biology and
Venue
Journal of Applied Physiology
Published
2000-07-01
Volume
89
Issue
1
Pages
251-258
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
OpenAlex
W2120756380
Cited by
97
References
33
Citations by year
2026: 1 · 2025: 2 · 2024: 6 · 2023: 6 · 2022: 5 · 2021: 1 · 2020: 2 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 3
FWCI
1.57
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01de011536-07)
  • National Institute of Dental and Craniofacial Research (DE11536)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
myosin heavy chain, masseter muscle, sexual dimorphism, androgens, testosterone, masticatory muscles, type IIA fibers, type IIb muscle fibers, muscle fiber composition, castration, ovariectomy, C57 mice
MeSH terms
Age Factors, Animals, Antibody Specificity, Female, Immunohistochemistry, Male, Masseter Muscle, Mice, Inbred C57BL, Orchiectomy, Ovariectomy, Sex Characteristics, Blotting, Western, Myosin Heavy Chains, Mice

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