Sexually dimorphic expression of myosin heavy chains in the adult mouse masseter
Abstract
Little is known regarding the role of androgenic hormones in the maintenance of myosin heavy chain (MHC) composition of rodent masticatory muscles. Because the masseter is the principal jaw closer in rodents, we felt it was important to characterize the influence of androgenic hormones on the MHC composition of the masseter. To determine the extent of sexual dimorphism in the phenotype of masseter muscle fibers of adult (10-mo-old) C57 mice, we stained tissue sections with antibodies specific to type IIa and IIb MHC isoforms. Females contain twice as many fibers containing the IIa MHC as males, and males contain twice as many fibers containing the IIb MHC as females. There is a modest amount of regionalization of MHC phenotypes in the mouse masseter. The rostral portions of the masseter are composed mostly of type IIa fibers, whereas the midsuperficial and caudal regions contain mostly type IIb fibers. Using immunoblots, we showed that castration results in an increase in the expression of type IIa MHC fibers in males. Ovariectomy has no effect on the fiber type composition in females. We conclude that testosterone plays a role in the maintenance of MHC expression in the adult male mouse masseter.
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The mouse masseter is composed of high numbers of IIX fibers in both sexes; however, there are threefold fewer IIB fibers and more IIA fibers in females compared with males.
“The mouse masseter is composed of high numbers of IIX fibers in both sexes; however, there are threefold fewer IIB fibers and more IIA fibers in females compared with males (27).”
From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [27]
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Motor Units and Muscle Receptors
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- Authors
- Eason, J. M., Schwartz, G. A., Pavlath, G. K., & English, A. W.
- Affiliations
- Emory University (US)
- Departments of Cell Biology and
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 2000-07-01
- Volume
- 89
- Issue
- 1
- Pages
- 251-258
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2120756380
- Cited by
- 97
- References
- 33
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 6 · 2023: 6 · 2022: 5 · 2021: 1 · 2020: 2 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01de011536-07)
- National Institute of Dental and Craniofacial Research (DE11536)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- myosin heavy chain, masseter muscle, sexual dimorphism, androgens, testosterone, masticatory muscles, type IIA fibers, type IIb muscle fibers, muscle fiber composition, castration, ovariectomy, C57 mice
- MeSH terms
- Age Factors, Animals, Antibody Specificity, Female, Immunohistochemistry, Male, Masseter Muscle, Mice, Inbred C57BL, Orchiectomy, Ovariectomy, Sex Characteristics, Blotting, Western, Myosin Heavy Chains, Mice
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