The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress
Abstract
Endothelial progenitor cells (EPCs), circulating in peripheral blood, migrate toward target tissue, differentiate, and contribute to the formation of new vessels. In this study, we report that shear stress generated by blood flow or tissue fluid flow can accelerate the proliferation, differentiation, and capillary-like tube formation of EPCs. When EPCs cultured from human peripheral blood were subjected to laminar shear stress, the cells elongated and oriented their long axes in the direction of flow. The cell density of the EPCs exposed to shear stress was higher, and a larger percentage of these cells were in the G 2-M phase of the cell cycle, compared with EPCs cultured under static conditions. Shear stress markedly increased the EPC expression of two vascular endothelial growth factor receptors, kinase insert domain-containing receptor and fms-like tyrosine kinase-1, and an intercellular adhesion molecule, vascular endothelial-cadherin, at both the protein and mRNA levels. Assays for tube formation in the collagen gels showed that the shear-stressed EPCs formed tubelike structures and developed an extensive tubular network significantly faster than the static controls. These findings suggest that EPCs are sensitive to shear stress and that their vasculogenic activities may be modulated by shear stress.
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Isolation of Putative Progenitor Endothelial Cells for Angiogenesis
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Self-citationFlow-mediated endothelial mechanotransduction
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Self-citationEndothelial Progenitor Cells: Characterization and Role in Vascular Biology
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Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization
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Buchanan, C. F., Verbridge, S. S., Vlachos, P. P., & Rylander, M. N. (2014) · Cell Adhesion & Migration · doi:10.4161/19336918.2014.970001 · Cited by 205
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VeDepo, M. C., Detamore, M. S., Hopkins, R. A., & Converse, G. L. (2017) · Journal of Tissue Engineering · doi:10.1177/2041731417726327 · Cited by 117
Construction of sinusoid-scale microvessels in perfusion culture of a decellularized liver
Watanabe, M., Yano, K., Okawa, K., Yamashita, T., Tajima, K., Sawada, K., Yagi, H., Kitagawa, Y., Tanishita, K., & Sudo, R. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.12.042 · Cited by 38
Clonal Expansion in Cardiovascular Pathology
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Metadata
- Authors
- Yamamoto, K., Takahashi, T., Asahara, T., Ohura, N., Sokabe, T., Kamiya, A., & Ando, J.
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 2003-11
- Volume
- 95
- Issue
- 5
- Pages
- 2081-2088
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2138900954
- Cited by
- 316
- References
- 46
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yamamoto, K., Takahashi, T., Asahara, T., Ohura, N., Sokabe, T., Kamiya, A., & Ando, J. (2003). Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress. Journal of Applied Physiology. https://doi.org/10.1152/japplphysiol.00232.2003
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