Cited by 103Exercise and Physiological ResponsesGenomics, phytochemicals, and oxidative stressMuscle Physiology and Disorders

Sulforaphane treatment protects skeletal muscle against damage induced by exhaustive exercise in rats

Malaguti, Marco, Angeloni, Cristina, Garatachea, Nuria, Baldini, Marta, Leoncini, Emanuela, Collado, Pilar S., Teti, Gabriella, Falconi, Mirella +2 more·2009·Journal of Applied Physiology

Abstract

Sulforaphane (SF), one of the most important isothiocyanates in the human diet, present in cruciferous vegetables, is known to have chemopreventive activities in different tissues. No data are available on its effects in the prevention of skeletal muscle damage. In this study, we investigated the potential protective effects of SF treatment on muscle damage and oxidative stress induced by an acute bout of exhaustive exercise in rats. Male Wistar rats were treated with SF (25 mg/kg body wt ip) for 3 days before undergoing an acute exhaustive exercise protocol in a treadmill (+7% slope and 24 m/min). Acute exercise resulted in a significant increase in plasma lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) activities. It also resulted in a significant increase in thiobarbituric acid-reactive substances, in a significant decrease in tissue total antioxidant capacity, and in a significant decrease in NAD(P)H:quinone oxidoreductase 1 (NQO1) expression and activity in vastus lateralis muscle. SF treatment significantly increased muscle NQO1, glutathione- S-transferase, and glutathione reductase expression and activity, with no effect on glutathione peroxidase and thioredoxin reductase. The observed SF-induced upregulation of phase II enzymes was accompanied by a significant increase in nuclear erythroid 2 p45-related factor 2 expression and correlated with a significant increase in total antioxidant capacity and a decrease in plasma LDH and CPK activities. Our data demonstrate that SF acts as an indirect antioxidant in skeletal muscle and could play a critical role in the modulation of the muscle redox environment, leading to the prevention of exhaustive exercise-induced muscle damage.

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Crossref · 10.1152/japplphysiol.00293.2009

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Authors
Malaguti, M., Angeloni, C., Garatachea, N., Baldini, M., Leoncini, E., Collado, P. S., Teti, G., Falconi, M., Gonzalez-Gallego, J., & Hrelia, S.
Affiliations
  • University of Bologna (IT)
  • Universidad de León (ES)
  • Institute of Biomedicine, University of León, León, Spain; and
Corresponding author
Hrelia, Silvana
Venue
Journal of Applied Physiology
Published
2009-10
Volume
107
Issue
4
Pages
1028-1036
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
OpenAlex
W2171906474
Cited by
103
References
64
Citations by year
2026: 7 · 2025: 5 · 2024: 7 · 2023: 5 · 2022: 5 · 2021: 7 · 2020: 12 · 2019: 4 · 2018: 7 · 2017: 8 · 2016: 8 · 2015: 8 · 2014: 5 · 2013: 6 · 2012: 4
FWCI
3.05
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
  • Unicam Scientific Publications (University of Camerino) (submittedVersion)
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
sulforaphane, isothiocyanates, skeletal muscle injury, exhaustive exercise, oxidative stress, NQO1, phase II enzymes, antioxidant capacity, Nrf2, glutathione S-transferase, glutathione reductase, creatine kinase, lactate dehydrogenase, chemoprevention
MeSH terms
Animals, Antioxidants, Creatine Kinase, Physical Exertion, Glutathione Peroxidase, Glutathione Reductase, Glutathione Transferase, L-Lactate Dehydrogenase, Male, Muscular Diseases, Oxidation-Reduction, Sulfoxides, Thiocyanates, Thioredoxin-Disulfide Reductase, NAD(P)H Dehydrogenase (Quinone), Rats, Wistar, Thiobarbituric Acid Reactive Substances, Isothiocyanates, Oxidative Stress, NF-E2-Related Factor 2, Rats, Quadriceps Muscle

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