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Cited by 349Ion channel regulation and functionIon Channels and ReceptorsCalcium signaling and nucleotide metabolism

The DOI matches its Crossref record; 424 of the 426 references it cites were found in a registry or an index.

Calcium Signaling and Exocytosis in Adrenal Chromaffin Cells

García, Antonio G., García-De-Diego, Antonio M., Gandía, Luis, Borges, Ricardo, García-Sancho, Javier·2006·Physiological Reviews
DOI 10.1152/physrev.00039.2005
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Abstract

At a given cytosolic domain of a chromaffin cell, the rate and amplitude of the Ca2+ concentration ([Ca2+]c) depends on at least four efficient regulatory systems: 1) plasmalemmal calcium channels, 2) endoplasmic reticulum, 3) mitochondria, and 4) chromaffin vesicles. Different mammalian species express different levels of the L, N, P/Q, and R subtypes of high-voltage-activated calcium channels; in bovine and humans, P/Q channels predominate, whereas in felines and murine species, L-type channels predominate. The calcium channels in chromaffin cells are regulated by G proteins coupled to purinergic and opiate receptors, as well as by voltage and the local changes of [Ca2+]c. Chromaffin cells have been particularly useful in studying calcium channel current autoregulation by materials coreleased with catecholamines, such as ATP and opiates. Depending on the preparation (cultured cells, adrenal slices) and the stimulation pattern (action potentials, depolarizing pulses, high K+, acetylcholine), the role of each calcium channel in controlling catecholamine release can change drastically. Targeted aequorin and confocal microscopy shows that Ca2+ entry through calcium channels can refill the endoplasmic reticulum (ER) to nearly millimolar concentrations, and causes the release of Ca2+ (CICR). Depending on its degree of filling, the ER may act as a sink or source of Ca2+ that modulates catecholamine release. Targeted aequorins with different Ca2+ affinities show that mitochondria undergo surprisingly rapid millimolar Ca2+ transients, upon stimulation of chromaffin cells with ACh, high K+, or caffeine. Physiological stimuli generate [Ca2+]c microdomains in which the local subplasmalemmal [Ca2+]c rises abruptly from 0.1 to approximately 50 microM, triggering CICR, mitochondrial Ca2+ uptake, and exocytosis at nearby secretory active sites. The fact that protonophores abolish mitochondrial Ca2+ uptake, and increase catecholamine release three- to fivefold, support the earlier observation. This increase is probably due to acceleration of vesicle transport from a reserve pool to a ready-release vesicle pool; this transport might be controlled by Ca2+ redistribution to the cytoskeleton, through CICR, and/or mitochondrial Ca2+ release. We propose that chromaffin cells have developed functional triads that are formed by calcium channels, the ER, and the mitochondria and locally control the [Ca2+]c that regulate the early and late steps of exocytosis.

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Research works printed but not linked: 2

  1. 10.1016/S0166-2236(97)87401-2 · DOI
  2. Hoffman BBand Taylor P.Neurotransmission. In:Goodman’s and Gilman’s: The Pharmacological Basis of Therapeutics(10th ed.), edited by Hardman JG, Limbird LE, and Goodman Gilman A. New York: McGraw-Hill, 2001, p. 115–153.

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Metadata

Authors
García, A. G., García-De-Diego, A. M., Gandía, L., Borges, R., & García-Sancho, J.
ORCID
Borges, Ricardo
Venue
Physiological Reviews
Publisher
American Physiological Society
Published
2006-10
Volume
86
Issue
4
Pages
1093-1131
ISSN
  • 0031-9333 (ISSN-L)
  • 1522-1210
DOI
10.1152/physrev.00039.2005
OpenAlex
W2137229430
Cited by
349
References
426
Citations by year
2026: 6 · 2025: 9 · 2024: 14 · 2023: 10 · 2022: 22 · 2021: 9 · 2020: 15 · 2019: 13 · 2018: 16 · 2017: 22 · 2016: 11 · 2015: 12 · 2014: 18 · 2013: 19 · 2012: 23
FWCI
1.91
Type
journal article
Language
en
Open access
closed
Locations
  • Physiological Reviews (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
calcium signaling, exocytosis, catecholamine release, calcium channels, P/Q-type calcium channels, L-type calcium channels, calcium-induced calcium release, endoplasmic reticulum, mitochondrial calcium uptake, chromaffin granules, opioid receptors, purinergic receptors, G proteins, aequorin, vesicle trafficking, cytoskeleton, adrenal medulla, confocal microscopy
MeSH terms
Adrenal Glands, Animals, Exocytosis, Humans, Chromaffin Cells, Calcium Signaling

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