Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse
Open accessAbstract
A number of calcium buffers were examined for their ability to reduce evoked transmitter release when injected into the presynaptic terminal of the squid giant synapse. Injection of EGTA was virtually ineffective at reducing transmitter release, even at estimated intracellular concentrations up to 80 mM. Conversely, the buffer 1,2-bis(2- aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA), which has an equilibrium affinity for calcium similar to that of EGTA at pH 7.2, produced a substantial reduction in transmitter release when injected presynaptically. This effect of BAPTA was reversible, presumably because the buffer diffused out of the terminal and into uninjected regions of the presynaptic axon. BAPTA derivatives with estimated intracellular calcium dissociation constants (Kd) ranging from 0.18 to 4.9 microM were effective at reducing transmitter release at similar estimated concentrations. A BAPTA derivative with an estimated intracellular Kd of 31 mM was less effective. BAPTA did not affect presynaptic action potentials or calcium spikes in ways that could explain its ability to reduce transmitter release. The relative effects of presynaptic injections of BAPTA and derivatives are consistent with the calcium-buffering capability of these compounds if the presynaptic calcium transient that triggers release is hundreds of microM or larger. The superior potency of BAPTA compared to EGTA apparently results from the faster calcium-binding kinetics of BAPTA and suggests that the calcium-binding molecule that triggers release binds calcium in considerably less than 200 microsec and is located very close to calcium channels.
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Biophysical measurements report the coupling distance between Ca2+ channels and release sensors, but the physical correlate of this measure remains undefined.
“Furthermore, biophysical measurements report the coupling distance between Ca2+ channels and release sensors [7,8] but the physical correlate of this measure remains undefined.”
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Research works printed but not linked: 1
- Augustine GJ, Buchanan J, Charlton MP, Osses LR, Smith SJ (1989) Fingering the trigger for neurotransmitter secretion: studies on the calcium channels of squid giant presynaptic terminals. In: Secretion and its control (Oxford GS, Armstrong CM, eds), pp 203-223. New York: Rockefeller UP.
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“Furthermore, biophysical measurements report the coupling distance between Ca 2+ channels and release sensors [7,8] but the physical correlate of this measure remains undefined.”
- Authors
- Adler, E., Augustine, G., Duffy, S., & Charlton, M.
- Affiliations
- University of Toronto (CA)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1991-06-01
- Volume
- 11
- Issue
- 6
- Pages
- 1496-1507
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6575403
- OpenAlex
- W1520756199
- Cited by
- 624
- References
- 1
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 17 · 2023: 12 · 2022: 13 · 2021: 6 · 2020: 9 · 2019: 15 · 2018: 8 · 2017: 7 · 2016: 9 · 2015: 19 · 2014: 18 · 2013: 14 · 2012: 18
- FWCI
- 11.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Toronto
- National Institutes of Health
- Medical Research Council
- National Institute of Neurological Disorders and Stroke (NS21624, R01NS021624)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- calcium chelators, BAPTA, EGTA, neurotransmitter release, squid giant synapse, presynaptic terminals, calcium buffering, calcium binding kinetics, calcium channels, calcium spikes
- MeSH terms
- Animals, Buffers, Calcium, Chelating Agents, Edetic Acid, Egtazic Acid, Iontophoresis, Kinetics, Synapses, Neurotransmitter Agents, Decapodiformes
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