The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy
Open accessAbstract
Cell‐based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self‐renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC‐based regenerative therapies. A number of complex inter‐ and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O 2 concentration (20%) in contrast to their niche where they usually reside in 2–9% O 2. Notably, O 2 plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia‐inducible factor‐1 (HIF‐1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O 2) and hypoxia (2–9% O 2) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC‐based regenerative therapies.
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The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis
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Extension of Life-Span by Introduction of Telomerase into Normal Human Cells
Bodnar, A. G., Ouellette, M., Frolkis, M., Holt, S. E., Chiu, C.-P., Morin, G. B., Harley, C. B., Shay, J. W., Lichtsteiner, S., & Wright, W. E. (1998) · Science · doi:10.1126/science.279.5349.349 · Cited by 4,975
HIFα Targeted for VHL-Mediated Destruction by Proline Hydroxylation: Implications for O 2 Sensing
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Human mesenchymal stem cells modulate allogeneic immune cell responses
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A perivascular origin for mesenchymal stem cells in multiple human organs
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BackgroundMesenchymal stem cells as trophic mediators
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Hypoxia requires Notch signaling to maintain the undifferentiated cell state
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Cited by
Mesenchymal stem cell secretome: toward cell-free therapeutic strategies in regenerative medicine
Vizoso, F., Eiro, N., Cid, S., Schneider, J., & Perez-Fernandez, R. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18091852 · Cited by 1,363
Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies
Noronha, N. D. C., Mizukami, A., Caliári-Oliveira, C., Cominal, J. G., Rocha, J. L. M., Covas, D. T., Swiech, K., & Malmegrim, K. C. R. (2019) · Stem Cell Research & Therapy · doi:10.1186/s13287-019-1224-y · Cited by 623
Concise Review: MSC Adhesion Cascade—Insights into Homing and Transendothelial Migration
Nitzsche, F., Müller, C., Lukomska, B., Jolkkonen, J., Deten, A., & Boltze, J. (2017) · Stem Cells · doi:10.1002/stem.2614 · Cited by 382
Yoon, D. S., Choi, Y., & Lee, J. W. (2016) · Cell Death & Disease · doi:10.1038/cddis.2016.3 · Cited by 105
Metadata
- Authors
- Haque, N., Rahman, M. T., Abu Kasim, N. H., & Alabsi, A. M.
- Venue
- The Scientific World Journal
- Publisher
- Wiley
- Published
- 2013-01
- Volume
- 2013
- Issue
- 1
- Pages
- 632972-632972
- ISSN
- 1537-744X (ISSN-L)
- 2356-6140
- OpenAlex
- W2169664936
- Cited by
- 230
- References
- 18
- FWCI
- 7.84
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
