Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Saponins as cytotoxic agents: a review

Podolak, I., Galanty, A., & Sobolewska, D. (2010) · Phytochemistry Reviews · doi:10.1007/s11101-010-9183-z · Cited by 718

Extraction and quantification of saponins: A review

Cheok, C. Y., Salman, H. A. K., & Sulaiman, R. (2014) · Food Research International · doi:10.1016/j.foodres.2014.01.057 · Cited by 450

Anticancer steroids: linking natural and semi-synthetic compounds

Salvador, J. A. R., Carvalho, J. F. S., Neves, M. A. C., Silvestre, S., Leitão, A. J., Silva, M. M., & Melo, M. L. S. E. (2012) · Natural Product Reports · doi:10.1039/c2np20082a · Cited by 283

Antioxidative and Hypolipidemic Effects of Diosgenin, a Steroidal Saponin of Yam (Dioscorea spp.), on High-Cholesterol Fed Rats

Son, I. S., Kim, J. H., Sohn, H. Y., Son, K. H., Kim, J., & Kwon, C.-S. (2007) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.70472 · Cited by 243

Diosgenin, a Steroidal Saponin, Inhibits Migration and Invasion of Human Prostate Cancer PC-3 Cells by Reducing Matrix Metalloproteinases Expression

Chen, P.-S., Shih, Y., Huang, H.-C., & Cheng, H.-W. (2011) · PLoS ONE · doi:10.1371/journal.pone.0020164 · Cited by 225

Antifungal Activity of C-27 Steroidal Saponins

Yang, C.-R., Zhang, Y., Jacob, M. R., Khan, S. I., Zhang, Y.-J., & Li, X.-C. (2006) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.50.5.1710-1714.2006 · Cited by 221

High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery

Attimarad, M., Ahmed, K. M., Al‐Dhubiab, B. E., & Sreeharsha, N. (2011) · Pharmaceutical Methods · doi:10.4103/2229-4708.84436 · Cited by 211

Cancer Chemopreventive and Therapeutic Effects of Diosgenin, a Food Saponin

Raju, J., & Mehta, R. (2009) · Nutrition and Cancer · doi:10.1080/01635580802357352 · Cited by 210

Diosgenin, a steroidal saponin, inhibits STAT3 signaling pathway leading to suppression of proliferation and chemosensitization of human hepatocellular carcinoma cells

Li, F., Fernandez, P. P., Rajendran, P., Hui, K. M., & Sethi, G. (2010) · Cancer Letters · doi:10.1016/j.canlet.2009.12.003 · Cited by 207

Cancer cell signaling pathways targeted by spice-derived nutraceuticals

Sung, B., Prasad, S., Yadav, V. R., & Aggarwal, B. B. (2012) · Nutrition and Cancer · doi:10.1080/01635581.2012.630551 · Cited by 204

Advances in the pharmacological activities and mechanisms of diosgenin

Chen, Y., Tang, Y., Yu, S., Han, Y., Kou, J., Liu, B., & Yu, B. (2015) · Chinese Journal of Natural Medicines · doi:10.1016/s1875-5364(15)30053-4 · Cited by 192

Oxidative stress in HIV demented patients and protection ex vivo with novel antioxidants

Turchan-Cholewo, J. T., Pocernich, C. B., Gairola, C. G., Chauhan, A., Schifitto, G., Butterfield, D. A., Buch, S. J., Narayan, O., Sinai, A. P., Geiger, J. D., et al. (2003) · Neurology · doi:10.1212/01.wnl.0000042048.85204.3d · Cited by 186

Diosgenin induces cell cycle arrest and apoptosis in human leukemia K562 cells with the disruption of Ca2+ homeostasis

Liu, M., Wang, Z., Ju, Y., Wong, R. N., & Wu, Q. (2004) · Cancer Chemotherapy and Pharmacology · doi:10.1007/s00280-004-0849-3 · Cited by 181

Diosgenin targets Akt‐mediated prosurvival signaling in human breast cancer cells

Srinivasan, S., Koduru, S. V., Kumar, R., Venguswamy, G., Kyprianou, N., & Damodaran, C. (2009) · International Journal of Cancer · doi:10.1002/ijc.24419 · Cited by 159

Antineoplastic and Apoptotic Potential of Traditional Medicines Thymoquinone and Diosgenin in Squamous Cell Carcinoma

Das, S., Dey, K. K., Dey, G., Pal, I., Majumder, A., Choudhury, S. M., Kundu, S. C., & Mandal, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0046641 · Cited by 158

Different contribution of apoptosis to the antiproliferative effects of diosgenin and other plant steroids, hecogenin and tigogenin, on human 1547 osteosarcoma cells

Corbière, C., Liagre, B., Bianchi, A., Bordji, K., Dauça, M., Netter, P., & Beneytout, J. (2003) · International Journal of Oncology · doi:10.3892/ijo.22.4.899 · Cited by 153

Diosgenin from Dioscorea bulbifera: Novel Hit for Treatment of Type II Diabetes Mellitus with Inhibitory Activity against α-Amylase and α-Glucosidase

Ghosh, S., More, P. A., Derle, A., Patil, A. B., Markad, P. R., Asok, A., Kumbhar, N., Shaikh, M. L., Boppana, R., Shinde, V., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0106039 · Cited by 151

Steroidal saponins from Smilax china and their anti-inflammatory activities

Shao, B., Guo, H., Cui, Y., Ye, M., Han, J., & Guo, D. (2006) · Phytochemistry · doi:10.1016/j.phytochem.2006.10.026 · Cited by 150

Diosgenin present in fenugreek improves glucose metabolism by promoting adipocyte differentiation and inhibiting inflammation in adipose tissues

Uemura, T., Hirai, S., Mizoguchi, N., Goto, T., Lee, J., Taketani, K., Nakano, Y., Shono, J., Hoshino, S., Tsuge, N., et al. (2010) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200900609 · Cited by 150

Diosgenin, 4-Hydroxyisoleucine, and Fiber from Fenugreek: Mechanisms of Actions and Potential Effects on Metabolic Syndrome

Fuller, S. E., & Stephens, J. M. (2015) · Advances in Nutrition · doi:10.3945/an.114.007807 · Cited by 147

A review on pharmacological and analytical aspects of diosgenin: a concise report

Patel, K., Gadewar, M. M., Tahilyani, V., & Patel, D. K. (2012) · Natural Products and Bioprospecting · doi:10.1007/s13659-012-0014-3 · Cited by 144

Diosgenin, the Main Aglycon of Fenugreek, Inhibits LXRα Activity in HepG2 Cells and Decreases Plasma and Hepatic Triglycerides in Obese Diabetic Mice

Uemura, T., Goto, T., Kang, M., Mizoguchi, N., Hirai, S., Lee, J.-Y., Nakano, Y., Shono, J., Hoshino, S., Taketani, K., et al. (2010) · Journal of Nutrition · doi:10.3945/jn.110.125591 · Cited by 143

Diosgenin induces death receptor-5 through activation of p38 pathway and promotes TRAIL-induced apoptosis in colon cancer cells

Lepage, C., Leger, D. Y., Bertrand, J., Martin, F., Beneytout, J., & Liagre, B. (2010) · Cancer Letters · doi:10.1016/j.canlet.2010.12.003 · Cited by 141

Anti-thrombosis effect of diosgenin extract from Dioscorea zingiberensis C.H. Wright in vitro and in vivo

Gong, G., Yuan, Q., & Huang, W. Q. (2010) · Phytomedicine · doi:10.1016/j.phymed.2010.08.015 · Cited by 128

Diosgenin inhibits macrophage-derived inflammatory mediators through downregulation of CK2, JNK, NF-κB and AP-1 activation

Jung, D., Park, H., Byun, H.-E., Park, Y.-M., Kim, T., Kim, B.-O., Um, S.-H., & Pyo, S. (2010) · International Immunopharmacology · doi:10.1016/j.intimp.2010.06.004 · Cited by 119

Antifungal Steroid Saponins fromDioscorea cayenensis

Sautour, M., Mitaine‐Offer, A., Miyamoto, T., Dongmo, A. B., & Lacaille‐Dubois, M. (2004) · Planta Medica · doi:10.1055/s-2004-815467 · Cited by 116

Efficacy of natural diosgenin on cardiovascular risk, insulin secretion, and beta cells in streptozotocin (STZ)-induced diabetic rats

Kalailingam, P., Kannaian, B., Eevera, T., & Kaliaperumal, R. (2014) · Phytomedicine · doi:10.1016/j.phymed.2014.04.005 · Cited by 112

NMR spectroscopy of steroidal sapogenins and steroidal saponins: An update

Agrawal, P. K., Jain, D. C., & Pathak, A. K. (1995) · Magnetic Resonance in Chemistry · doi:10.1002/mrc.1260331202 · Cited by 111

Diosgenin Functionalized Iron Oxide Nanoparticles as Novel Nanomaterial Against Breast Cancer

Ghosh, S., More, P. A., Derle, A., Kitture, R., Kale, T., Gorain, M., Avasthi, A., Markad, P. R., Kundu, G. C., Kale, S. N., et al. (2015) · Journal of Nanoscience and Nanotechnology · doi:10.1166/jnn.2015.11704 · Cited by 111

Diosgenin inhibits the migration of human breast cancer MDA-MB-231 cells by suppressing Vav2 activity

He, Z., Chen, H., Li, G., Zhu, H., Gao, Y., Zhang, L., & Sun, J. (2014) · Phytomedicine · doi:10.1016/j.phymed.2014.02.002 · Cited by 106

Diosgenin induces ROS-dependent autophagy and cytotoxicity via mTOR signaling pathway in chronic myeloid leukemia cells

Jiang, S., Fan, J., Wang, Q., Ju, D., Feng, M., Li, J., Guan, Z.-B., An, D., Wang, X., & Li, Y. (2016) · Phytomedicine · doi:10.1016/j.phymed.2016.01.010 · Cited by 101

Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS

Yi, T., Fan, L., Chen, H., Zhu, G., Hau‐Man, S., Tang, Y., Chu, C., Chu, C., Zhao, Z., & Chen, H. (2014) · BMC Biochemistry · doi:10.1186/1471-2091-15-19 · Cited by 97

Inhibition of TNF-α-induced adhesion molecule expression by diosgenin in mouse vascular smooth muscle cells via downregulation of the MAPK, Akt and NF-κB signaling pathways

Choi, K.-W., Park, H., Jung, D., Kim, T., Park, Y.-M., Kim, B.-O., Sohn, E., Moon, E., Um, S. H., Rhee, D., et al. (2010) · Vascular Pharmacology · doi:10.1016/j.vph.2010.09.007 · Cited by 88

Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway

Kim, D. S., Jeon, B. K., Lee, Y. E., Woo, W.-H., & Mun, Y. J. (2012) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2012/981675 · Cited by 88

Diosgenin inhibits atherosclerosis via suppressing the MiR-19b-induced downregulation of ATP-binding cassette transporter A1

Lv, Y.-C., Yang, J., Yao, F., Xie, W., Tang, Y., Ouyang, X.-P., He, P., Tan, Y.-L., Li, L., Zhang, M., et al. (2015) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2015.02.044 · Cited by 85

UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

Tang, Y., Pang, Y., He, X.-C., Zhang, Y., Zhang, J., Zhao, Z., Yi, T., & Chen, H. (2015) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2015.02.017 · Cited by 85

Microdetermination of Diosgenin from Fenugreek (Trigonella foenum-graecum) Seeds

Taylor, W. G., Elder, J. L., Chang, P. R., & Richards, K. W. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000467t · Cited by 85

Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm

Selim, S., & Jaouni, S. K. A. (2015) · BMC Complementary and Alternative Medicine · doi:10.1186/s12906-015-0836-8 · Cited by 83

Effect of liquid crystals with cyclodextrin on the bioavailability of a poorly water-soluble compound, diosgenin, after its oral administration to rats

Okawara, M., Hashimoto, F., Todo, H., Sugibayashi, K., & Tokudome, Y. (2014) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2014.06.032 · Cited by 75

Diosgenin ameliorates palmitate-induced endothelial dysfunction and insulin resistance via blocking IKKβ and IRS-1 pathways

Liu, K., Zhao, W., Gao, X., Huang, F., Kou, J., & Liu, B. (2012) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2012.06.012 · Cited by 72

Analysis of Steroidal Sapogenins from Amber Fenugreek (Trigonella foenum-graecum) by Capillary Gas Chromatography and Combined Gas Chromatography/Mass Spectrometry

Taylor, W. G., Zaman, M. S., Mir, Z., Mir, P. S., Acharya, S. N., Mears, G. J., & Elder, J. L. (1997) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf960200n · Cited by 72

Synthesis of novel anticancer agents through opening of spiroacetal ring of diosgenin

Hamid, A. A., Hasanain, M., Singh, A., Bhukya, B., Omprakash, Vasudev, P. G., Sarkar, J., Chanda, D., Khan, F., Aiyelaagbe, O. O., et al. (2014) · Steroids · doi:10.1016/j.steroids.2014.05.025 · Cited by 72

Role of oxidative stress, inflammation, nitric oxide and transforming growth factor-beta in the protective effect of diosgenin in monocrotaline-induced pulmonary hypertension in rats

Ahmed, L. A., Obaid, A. A. Z., Zaki, H. F., & Agha, A. M. (2014) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2014.07.026 · Cited by 68

Diosgenin induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma cells

Li, Y., Wang, X., Cheng, S., Du, J., Deng, Z., Zhang, Y., LIU, Q., Gao, J., Cheng, B., & Ling, C. (2014) · Oncology Reports · doi:10.3892/or.2014.3629 · Cited by 68

Cytotoxicity and apoptosis-inducing effect of steroidal saponins from Dioscorea zingiberensis Wright against cancer cells

Tong, Q., He, Y., Zhao, Q., Qing, Y., Huang, W. Q., & Wu, X. (2012) · Steroids · doi:10.1016/j.steroids.2012.04.019 · Cited by 66

Diosgenin, a Plant-Derived Sapogenin, Exhibits Antiviral Activity in Vitro against Hepatitis C Virus

Wang, Y.-J., Pan, K., Hsieh, T.-C., Chang, T., Lin, W., & Hsu, J. T.-A. (2011) · Journal of Natural Products · doi:10.1021/np100578u · Cited by 66

Diosgenin Inhibits hTERT Gene Expression in the A549 Lung Cancer Cell Line

Mohammad, R. Y., Ghareghomi, S., Gholamreza, H., Mobasseri, M., & Rasmi, Y. (2013) · Asian Pacific Journal of Cancer Prevention · doi:10.7314/apjcp.2013.14.11.6945 · Cited by 61

Diosgenin-induced cognitive enhancement in normal mice is mediated by 1,25D3-MARRS

Tohda, C., Lee, Y., Goto, Y., & Nemere, I. (2013) · Scientific Reports · doi:10.1038/srep03395 · Cited by 60

Diosgenin regulates adipokine expression in perivascular adipose tissue and ameliorates endothelial dysfunction via regulation of AMPK

Chen, Y., Xu, X., Zhang, Y., Liu, K., Huang, F., Liu, B., & Kou, J. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2015.07.005 · Cited by 59

Characterization of in Vitro ADME Properties of Diosgenin and Dioscin from Dioscorea villosa

Manda, V. K., Avula, B., Ali, Z., Wong, Y.-H., Smillie, T. J., Khan, I. A., & Khan, S. I. (2013) · Planta Medica · doi:10.1055/s-0033-1350699 · Cited by 59

Diosgenin Attenuates Allergen-Induced Intestinal Inflammation and IgE Production in a Murine Model of Food Allergy

Huang, C., Ku, C.-Y., & Jan, T. (2009) · Planta Medica · doi:10.1055/s-0029-1185578 · Cited by 59

Fenugreek extract diosgenin and pure diosgenin inhibit the hTERT gene expression in A549 lung cancer cell line

Rahmati‐Yamchi, M., Ghareghomi, S., Gholamreza, H., Milani, M., Aghazadeh, M. H., & Daroushnejad, H. (2014) · Molecular Biology Reports · doi:10.1007/s11033-014-3505-y · Cited by 58

Variation in Diosgenin Levels among 10 Accessions of Fenugreek Seeds Produced in Western Canada

Taylor, W. G., Zulyniak, H. J., Richards, K. W., Acharya, S. N., Bittman, S., & Elder, J. L. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020486y · Cited by 57

Modulatory Effects of Diosgenin on Attenuating the Key Enzymes Activities of Carbohydrate Metabolism and Glycogen Content in Streptozotocin-Induced Diabetic Rats

Saravanan, G., Ponmurugan, P., Deepa, M. A., & Senthilkumar, B. (2014) · Canadian Journal of Diabetes · doi:10.1016/j.jcjd.2014.02.004 · Cited by 57

Diosgenin induces cell cycle arrest and apoptosis in HEL cells with increase in intracellular calcium level, activation of cPLA2 and COX-2 overexpression

Leger, D. Y., Liagre, B., Corbière, C., Cook‐Moreau, J., & Beneytout, J. (2004) · International Journal of Oncology · doi:10.3892/ijo.25.3.555 · Cited by 56

High-Dose Diosgenin Reduces Bone Loss in Ovariectomized Rats via Attenuation of the RANKL/OPG Ratio

Zhang, Z., Song, C., Fu, X., Liu, M., Li, Y., Pan, J., Liu, H., Wang, S., Xiang, L., Xiao, G. G., et al. (2014) · International Journal of Molecular Sciences · doi:10.3390/ijms150917130 · Cited by 56

Diosgenin prevents bone loss on retinoic acid-induced osteoporosis in rats

Zhao, S., Niu, F., Xu, C.-Y., Liu, Y., Ye, L., Bi, G.-B., Chen, L., Tian, G., & Nie, T.-H. (2015) · Irish Journal of Medical Science (1971 -) · doi:10.1007/s11845-015-1309-2 · Cited by 54

Diosgenin dose-dependent apoptosis and differentiation induction in human erythroleukemia cell line and sedimentation field-flow fractionation monitoring

Leger, D. Y., Liagre, B., Cardot, P. J. P., Beneytout, J., & Battu, S. (2004) · Analytical Biochemistry · doi:10.1016/j.ab.2004.09.008 · Cited by 54

Renoprotective effect of diosgenin in streptozotocin induced diabetic rats

Kanchan, D. M., Somani, G. S., Peshattiwar, V., Kaikini, A., & Sathaye, S. (2016) · Pharmacological Reports · doi:10.1016/j.pharep.2015.10.011 · Cited by 53

Anti-thrombosis effect of diosgenyl saponins in vitro and in vivo

Zhang, R., Huang, B., Du, D., Guo, X., Xin, G., Xing, Z., Liang, Y. K., Chen, Y., Chen, Q., He, Y., et al. (2013) · Steroids · doi:10.1016/j.steroids.2013.07.003 · Cited by 49

A more ecological and efficient approach for producing diosgenin from Dioscorea zingiberensis tubers via pressurized biphase acid hydrolysis

Yang, H., Yin, H., Shen, Y., Xia, G., Zhang, B., Wu, X., Cai, B., & Tam, J. P. (2016) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2016.05.030 · Cited by 49

Diosgenin attenuates inflammatory response induced by myocardial reperfusion injury: role of mitochondrial ATP-sensitive potassium channels

Ebrahimi, H., Badalzadeh, R., Mohammadi, M., & Yousefi, B. (2014) · Journal of Physiology and Biochemistry · doi:10.1007/s13105-014-0320-9 · Cited by 46

Development and validation of a sensitive and rapid non-aqueous LC–ESI-MS/MS method for measurement of diosgenin in the plasma of normal and hyperlipidemic rats: A comparative study

Xu, L., Liu, Y., Wang, T., Qi, Y., Han, X., Xu, Y., Peng, J., & Tang, X. (2009) · Journal of Chromatography B · doi:10.1016/j.jchromb.2009.03.039 · Cited by 45

Simultaneous analysis of diosgenin and sarsasapogenin in Asparagus officinalis byproduct by thin‐layer chromatography

Wang, L., Wang, X., Yuan, X., & Zhao, B. (2010) · Phytochemical Analysis · doi:10.1002/pca.1244 · Cited by 45

Anti-Proliferation and Anti-Invasion Effects of Diosgenin on Gastric Cancer BGC-823 Cells with HIF-1α shRNAs

Mao, Z.-J., Tang, Q., Zhang, C., Qin, Z., Pang, B., Wei, P.-K., Liu, B., & Chou, Y.-N. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13056521 · Cited by 45

Chemical fingerprint analysis and quantitative determination of steroidal compounds from Dioscorea villosa, Dioscorea species and dietary supplements using UHPLC‐ELSD

Avula, B., Wang, Y., Ali, Z., Smillie, T. J., & Khan, I. A. (2013) · Biomedical Chromatography · doi:10.1002/bmc.3019 · Cited by 44

Diosgenin Mitigates Streptozotocin Diabetes-induced Vascular Dysfunction of the Rat Aorta

Roghani, F., Roghani, M., & Baluchnejadmojarad, T. (2015) · Journal of Cardiovascular Pharmacology · doi:10.1097/fjc.0000000000000308 · Cited by 43

PPAR’s and Diosgenin a chemico biological insight in NIDDM

Sangeetha, M. K., Mal, N. S., Atmaja, K., Sali, V. K., & Vasanthi, H. R. (2013) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2013.08.014 · Cited by 43

Diosgenin, a plant steroid, induces apoptosis in COX-2 deficient K562 cells with activation of the p38 MAP kinase signalling and inhibition of NF-κB binding

Liagre, B., Bertrand, J., Leger, D. Y., & Beneytout, J. (2005) · International Journal of Molecular Medicine · doi:10.3892/ijmm.16.6.1095 · Cited by 42

Preparation of steroidal hormones with an emphasis on transformations of phytosterols and cholesterol - a review

Jasem, Y. A., Khan, M., Taha, A., & Thiemann, T. (2014) · Mediterranean Journal of Chemistry · doi:10.13171/mjc.3.2.2014.18.04.15 · Cited by 41

Microwave-assisted extraction and a new determination method for total steroid saponins from Dioscorea zingiberensis C.H. Wright

Ren, Y., Chen, Y., Hu, B., Wu, H., Lai, F., & Li, X. (2015) · Steroids · doi:10.1016/j.steroids.2015.09.008 · Cited by 40

A Validated Quantitative Thin-Layer Chromatographic Method for Estimation of Diosgenin in Various Plant Samples, Extract, and Market Formulation

Trivedi, P. D., Pundarikakshudu, K., Rathnam, S., & Shah, K. (2007) · Journal of AOAC International · doi:10.1093/jaoac/90.2.358 · Cited by 40

The effects of diosgenin in the Regulation of renal proximal tubular fibrosis

Wang, W., Liu, S., Chang, W., Shiue, Y., Hsieh, P., Hung, T., Hung, C., Hung, I. Y., Chen, M.-F., & Yang, Y. (2014) · Experimental Cell Research · doi:10.1016/j.yexcr.2014.01.028 · Cited by 39

Solasodine and diosgenin: 1H and 13C assignments by two‐dimensional NMR spectroscopy

Puri, R., Wong, T. C., & Puri, R. K. (1993) · Magnetic Resonance in Chemistry · doi:10.1002/mrc.1260310313 · Cited by 36

Diosgenin relieves goiter via the inhibition of thyrocyte proliferation in a mouse model of Graves' disease

Cai, H., Wang, Z., Zhang, H., Wang, F., Yu, C., Zhang, F., Gao, L., Zhang, J., & Zhao, J. (2013) · Acta Pharmacologica Sinica · doi:10.1038/aps.2013.133 · Cited by 36

Anti-arrhythmic effect of diosgenin in reperfusion-induced myocardial injury in a rat model: activation of nitric oxide system and mitochondrial KATP channel

Badalzadeh, R., Yousefi, B., Majidinia, M., & Ebrahimi, H. (2014) · The Journal of Physiological Sciences · doi:10.1007/s12576-014-0333-8 · Cited by 35

Synthesis, characterization and biological studies of diosgenyl analogues

Huang, B., Du, D., Zhang, R., Wu, X., Xing, Z., He, Y., & Huang, W. Q. (2012) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2012.10.086 · Cited by 35

Diosgenin inhibits IL-1β-induced expression of inflammatory mediators in human osteoarthritis chondrocytes.

Wang, L., Ma, T., Zheng, Y., Lv, S., Yu, L., & Liu, S. (2015) · PubMed Central · Cited by 35

Sequential release of drugs from hollow manganese ferrite nanocarriers for breast cancer therapy

Kumar, B. N. P., Puvvada, N., Rajput, S., Sarkar, S., Das, S. K., Emdad, L., Sarkar, D., Venkatesan, P., Pal, I., Dey, G., et al. (2014) · Journal of Materials Chemistry B · doi:10.1039/c4tb01098a · Cited by 33

Qualitative and Quantitative Analysis of Microbial Transformation of Steroidal Saponins in Dioscorea zingiberensis

Qi, S., Dong, Y., Zhao, Y., & Xiu, Z. (2009) · Chromatographia · doi:10.1365/s10337-009-0978-2 · Cited by 32

Metabolite profiling of saponins in Balanites aegyptiaca plant tissues using LC (RI)-ESI/MS and MALDI-TOF/MS

Chapagain, B. P., & Wiesman, Z. (2008) · Metabolomics · doi:10.1007/s11306-008-0129-z · Cited by 31

Diosgenin quantification by HPLC in a Dioscorea polygonoides tuber collection from colombian flora

Niño, J., Jiménez, D. A., Mosquera, O. M., & Correa-Navarro, Y. M. (2007) · Journal of the Brazilian Chemical Society · doi:10.1590/s0103-50532007000500030 · Cited by 30

Metabolism of diosgenin-derived saponins: implications for hepatogenous photosensitization diseases in ruminants

Meagher, L. P., Smith, B. L., & Wilkins, A. L. (2001) · Animal Feed Science and Technology · doi:10.1016/s0377-8401(01)00223-1 · Cited by 29

Antimicrobial and inhibition on heat-induced protein denaturation of constituents isolated fromPolygonatumverticillatumrhizomes

Khan, H. Z., Saeed, M., Rauf, A., Khan, M. A., & Muhammad, N. (2015) · Natural Product Research · doi:10.1080/14786419.2014.996150 · Cited by 29

Quantitative determination of diosgenin in Dioscorea zingiberensis cell cultures by microplate-spectrophotometry and high-performance liquid chromatography

Li, P. (2012) · African Journal of Pharmacy and Pharmacology · doi:10.5897/ajpp11.440 · Cited by 29

Fenugreek seed extract and its phytocompounds- trigonelline and diosgenin arbitrate their hepatoprotective effects through attenuation of endoplasmic reticulum stress and oxidative stress in type 2 diabetic rats

Tharaheswari, M., Nakkala, J. R., Jeepipalli, S. P. K., Raja, K., Chandra, V. K., Mohan, V. K., & Sadras, S. R. (2014) · European Food Research and Technology · doi:10.1007/s00217-014-2322-9 · Cited by 28

Isolation and characterization of diosgenin from in vitro cultured tissues of Helicteres isora L

Deshpande, H. A., & Bhalsing, S. R. (2013) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-013-0205-3 · Cited by 28

Influence of plant matrix on microwave‐assisted extraction process. The case of diosgenin extracted from fenugreek (Trigonella foenum‐graecum L.)

Kaufmann, B., Rudaz, S. L., Cherkaoui, S., Veuthey, J., & Christen, P. (2006) · Phytochemical Analysis · doi:10.1002/pca.954 · Cited by 28

Preventive effect of a novel diosgenin derivative on arterial and venous thrombosis in vivo

Zheng, H., Wei, Z., Xin, G., Ji, C., Wen, L., Xia, Q., Niu, H., & Huang, W. Q. (2016) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2016.05.032 · Cited by 28

Diosgenin-induced protection against myocardial ischaemia-reperfusion injury is mediated by mitochondrial K ATP channels in a rat model

Badalzadeh, R., Yousefi, B., Tajaddini, A., & Ahmadian, N. (2014) · Perfusion · doi:10.1177/0267659114566064 · Cited by 27

Diosgenin Modulates Vascular Smooth Muscle Cell Function by Regulating Cell Viability, Migration, and Calcium Homeostasis

Esfandiarei, M., Lam, J. T. N., Yazdi, S. A., Kariminia, A., Navarro‐Dorado, J., Kuzeljevic, B., Syyong, H. T., Hu, K., & van Breemen, C. (2010) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.110.172684 · Cited by 27

Development and Validation of HPTLC Method for Simultaneous Estimation of Diosgenin and Quercetin in Fenugreek Seeds (Trigonella foenum-graceum)

Laıla, O., Murtaza, I., Abdin, M. Z., Ahmad, S., Ganai, N. A., & Jehangir, M. (2014) · ISRN Chromatography · doi:10.1155/2014/583047 · Cited by 26

Effect of β-Cyclodextrin Derivatives on the Diosgenin Absorption in Caco-2 Cell Monolayer and Rats

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Effects of total saponins from Rhizoma Dioscoreae Nipponicae on expression of vascular endothelial growth factor and angiopoietin-2 and Tie-2 receptors in the synovium of rats with rheumatoid arthritis

Guo, Y., Xing, E., Liang, X., Song, H., & Dong, W. (2016) · Journal of the Chinese Medical Association · doi:10.1016/j.jcma.2015.10.012 · Cited by 26

Diosgenin inhibits tumor necrosis factor-induced tissue factor activity and expression in THP-1 cells via down-regulation of the NF-κB, Akt, and MAPK signaling pathways

Yang, H., Lei, Y. U. E., Jiang, W., Liu, Q., Kou, J., & Yu, B. (2013) · Chinese Journal of Natural Medicines · doi:10.1016/s1875-5364(13)60070-9 · Cited by 25

Newer Developments in HPLC Impacting Pharmaceutical Analysis : A Brief Review

Guillarme, D., & Dong, M. W. (2013) · Archive ouverte UNIGE (University of Geneva) · Cited by 24

Direct Biotransformation of Dioscin into Diosgenin in Rhizome of Dioscorea zingiberensis by Penicillium dioscin

Dong, J., Lei, C., Lu, D., & Wang, Y. (2014) · Indian Journal of Microbiology · doi:10.1007/s12088-014-0507-3 · Cited by 24

Diosgenin effectively suppresses skin inflammation induced by phthalic anhydride in IL-4/Luc/CNS-1 transgenic mice

Kim, J. E., Go, J., Koh, E. K., Song, S. H., Sung, J. E., Lee, H. A., Kim, D. S., Son, H. J., Lee, H. S., Lee, C. Y., et al. (2016) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2015.1135040 · Cited by 24

Simultaneous determination of furostanol and spirostanol glycosides in Taiwanese yam (Dioscorea spp.) cultivars by high performance liquid chromatography

Yang, D.-J., Lu, T., & Hwang, L. S. (2020) · Journal of Food and Drug Analysis · doi:10.38212/2224-6614.2675 · Cited by 24

Diosgenin prevents hepatic oxidative stress, lipid peroxidation and molecular alterations in chronic renal failure rats

Balamurugan, E., Manivannan, J., Sivasubramanian, J., & Arunagiri, P. (2013) · International journal of Nutrition Pharmacology Neurological Diseases · doi:10.4103/2231-0738.114870 · Cited by 22

Rapid quantitative analysis of diosgenin in the tubers of Dioscorea zingiberensis C.H. Wright by coupling cellulose enzymolysis and two-phase acid hydrolysis in tandem with HPLC-UV

Yang, H., Chen, B., Wang, X.-B., Chue, P., Shen, Y., Xia, G.-H., & Jia, X. (2013) · Natural Product Research · doi:10.1080/14786419.2013.790030 · Cited by 22

Diosgenin quantification, characterisation and chemical composition in a tuber collection of D ioscorea spp. in the state of J alisco, M exico

Contreras-Pacheco, M. L., Santacruz‐Ruvalcaba, F., García-Fajardo, J. A., José de Jesús Sánchez, G., Ruíz-López, M. A., Estarrón‐Espinosa, M., & Castro‐Castro, A. (2013) · International Journal of Food Science & Technology · doi:10.1111/ijfs.12193 · Cited by 22

Mitochondrial ATP-sensitive K+ channels mediate the antioxidative influence of diosgenin on myocardial reperfusion injury in rat hearts

Badalzadeh, R., Yavari, R., & Chalabiani, D. (2015) · General Physiology and Biophysics · doi:10.4149/gpb_2015009 · Cited by 22

A self-assembled nanoparticle platform based on poly(ethylene glycol)–diosgenin conjugates for co-delivery of anticancer drugs

Li, C., Dai, L., Liu, K., Deng, L., Pei, T., & Lei, J. (2015) · RSC Advances · doi:10.1039/c5ra15375a · Cited by 21

Efficient protocol for purification of diosgenin and two fatty acids from Rhizoma dioscoreae by SFE coupled with high‐speed counter‐current chromatography and evaporative light scattering detection

Xu, Y., Han, X., Dong, D., Xu, L., Qi, Y., Peng, J., & Zhan, L. (2008) · Journal of Separation Science · doi:10.1002/jssc.200800295 · Cited by 21

Determination of saponins in the kernel cake of Balanites aegyptiaca by HPLC‐ESI/MS

Chapagain, B. P., & Wiesman, Z. (2007) · Phytochemical Analysis · doi:10.1002/pca.990 · Cited by 21

LC–MSn characterization of steroidal saponins in Helleborus niger L. roots and their conversion products during fermentation

Duckstein, S. M., & Stintzing, F. C. (2014) · Steroids · doi:10.1016/j.steroids.2014.09.011 · Cited by 20

Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways

Lin, Y., Jia, R., Liu, Y., Gao, Y., Zeng, X., Kou, J., & Yu, B. (2014) · Free Radical Research · doi:10.3109/10715762.2014.966705 · Cited by 17

Steroidal saponin diosgenin from Dioscorea bulbifera protects cardiac cells from hypoxia -reoxygenation injury through modulation of pro -survival and pro -death molecules

Vasanthi, H. R., Jayachandran, K. S. P., & Gurusamy, N. (2016) · Pharmacognosy Magazine · doi:10.4103/0973-1296.176114 · Cited by 17

Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay

Li, J., Yang, D., Yu, K., He, J. L., & Zhang, Y. (2010) · Planta Medica · doi:10.1055/s-0030-1250054 · Cited by 14

Comparison between high performance liquid chromatography and high performance thin layer chromatography determination of Diosgenin from fenugreek seeds

Nagore, D. H., Patil, P., & Kuber, V. V. (2012) · International Journal of Green Pharmacy · doi:10.4103/0973-8258.108239 · Cited by 14

Quantitative determination of sarsasapogenin in rat plasma using liquid chromatography-tandem mass spectrometry

Yang, B., Liu, Z., Hu, J. Y., Lai, X., & Xia, P. (2016) · Journal of Chromatography B · doi:10.1016/j.jchromb.2016.04.020 · Cited by 13

Development and validation of a liquid chromatography/tandem mass spectrometry assay for the quantification of methyl protodioscin in rat plasma: application to a pharmacokinetic study

Cao, X.-Z., Yao, Z., Chen, H., Dai, Y., Sun, P., Ye, W., & Yao, X. (2007) · Biomedical Chromatography · doi:10.1002/bmc.948 · Cited by 12

Improving Bone Microarchitecture in Aging with Diosgenin Treatment: A Study in Senescence-Accelerated OXYS Rats

Tikhonova, M. A. (2015) · The Chinese Journal of Physiology · doi:10.4077/cjp.2015.bad325 · Cited by 12

Effect of Modified Enzymatic Catalysis on the Extraction of Diosgenin from Dioscorea zingiberensis C. H. Wright

Zhang, Y. Q., Liang, J. H., Fu, E. H., & Li, B. (2007) · Chemical Engineering & Technology · doi:10.1002/ceat.200700234 · Cited by 10

A sensitive indirect competitive enzyme-linked immunosorbent assay for the detection of sarsasapogenin in rat plasma

Wang, J., Liu, J.-H., Guo, X., Zhang, J., & Yu, B. (2010) · Acta Pharmacologica Sinica · doi:10.1038/aps.2010.85 · Cited by 9

The Effect of Crude Diosgenin Extract from Purple and Yellow Greater Yams (Dioscorea alata L.) on the Lipid Profile of Dyslipidemia Rats

Harijono, H., Estiasih, T., Ariestiningsih, A. D., & Wardani, N. (2016) · Emirates Journal of Food and Agriculture · doi:10.9755/ejfa.2016-01-086 · Cited by 9

Purification and characterization of a glycosidase with hydrolyzing multi-3-O-glycosides of spirostanol saponin activity from Gibberella intermedia

Li, H., Wang, X., Ma, Y., Yang, N., Zhang, X., Xu, Z., & Jinsong, S. (2016) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/j.molcatb.2016.03.003 · Cited by 8

Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extract (FSA)

Ahmed Kaid, N. A., Norbaiyah, M. B., Imad, M. A., & Norazian, M. H. (2016) · IIUM Medical Journal Malaysia · doi:10.31436/imjm.v15i1.410 · Cited by 8

ANTI-AMEBIC ACTIVITY OF DIOSGENIN ON NAEGLERIA FOWLERI TROPHOZOITES.

Rabablert, J., Tiewcharoen, S., Auewarakul, P., Atithep, T., Lumlerdkij, N., Vejaratpimol, R., & Junnu, V. (2015) · PubMed · Cited by 6

Development of a new certified reference material of diosgenin using mass balance approach and Coulometric titration method

Gong, N., Zhang, B., Hu, F., Du, H., Du, G., Gao, Z., & Lü, Y. (2014) · Steroids · doi:10.1016/j.steroids.2014.08.019 · Cited by 5

An Efficient Purification Method for Quantitative Determinations of Protodioscin, Dioscin and Diosgenin in Plasma of Fenugreek-Fed Mice

Taketani, K., Hoshino, S., Uemura, T., Goto, T., Takahashi, N., Tsuge, N., & Kawada, T. (2015) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.61.465 · Cited by 5

Quantitative Estimation of Piperine and Diosgenin in Ayurvedic formulation

Parameswaran, S. (2014) · Journal of Pharmaceutical Research International · doi:10.9734/bjpr/2014/8310 · Cited by 5

Supramolecular prodrug micelles based on the complementary multiple hydrogen bonds as drug delivery platform for thrombosis therapy

Li, W., Wang, H., Wei, Z., Ning, J., Ma, L., Zheng, H., Niu, H., & Huang, W. Q. (2016) · RSC Advances · doi:10.1039/c6ra11110f · Cited by 5

The Dioscorea Genus (Yam)—An Appraisal of Nutritional and Therapeutic Potentials

Obidiegwu, J. E., Lyons, J. B., & Chilaka, C. A. (2020) · Foods · doi:10.3390/foods9091304 · Cited by 288

Green synthesis and characterization of zinc oxide nanoparticle using insulin plant ( Costus pictus D. Don ) and investigation of its antimicrobial as well as anticancer activities

Suresh, J., Pradheesh, G., Alexramani, V., Sundrarajan, M., & Hong, S. I. (2018) · Advances in Natural Sciences Nanoscience and Nanotechnology · doi:10.1088/2043-6254/aaa6f1 · Cited by 254

Pro-Apoptotic and Anti-Cancer Properties of Diosgenin: A Comprehensive and Critical Review

Sethi, G., Shanmugam, M. K., Warrier, S. R., Mérarchi, M., Arfuso, F., Kumar, A. P., & Bishayee, A. (2018) · Nutrients · doi:10.3390/nu10050645 · Cited by 254

Repeated evolution of cytochrome P450-mediated spiroketal steroid biosynthesis in plants

Christ, B., Xu, C., Xu, M., Li, F., Wada, N., Mitchell, A. J., Han, X., Wen, M., Fujita, M., & Weng, J. (2019) · Nature Communications · doi:10.1038/s41467-019-11286-7 · Cited by 204

Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery: Insights, Avenues, and Challenges

Singh, A. K., Singh, D. K., Kharwar, R. N., White, J. F., & Gond, S. K. (2021) · Microorganisms · doi:10.3390/microorganisms9010197 · Cited by 198

Development and Validation of Sensitive HPLC-UV-DAD Method for Determination of Diosgenin in Plants of Species of Dioscorea Genus

da Silva, L. R. G., Cheibub, A. M. D. S. S., Valverde, A. L., & Netto, A. D. P. (2019) · Revista Virtual de Química · doi:10.21577/1984-6835.20190090

DIOSGENIN: AN INTEGRATED REVIEW OF CHEMICAL RESEARCH, ANALYTICAL TECHNIQUES, AI APPLICATIONS, AND SAFETY: A Comprehensive Review

Meghana, D., Bhavana, A., Kasthuri, B., Nikhila, A., Yaswanth, A., Chowdary, D. Y. A., & Madhuri, M. V. B. (2026) · Zenodo (CERN European Organization for Nuclear Research) · doi:10.5281/zenodo.23011253

Estimation of Diosgenin by HPLC in a Dioscorea zingiberensis Collection from Different Local Markets in Middle and South Iraq

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Development and Validation of an HPLC Method for Diosgenin, A Bioactive Compound in the Rhizomes of Chamaecostus cuspidatus

Saliya, H., Madam, J., Patel, H. D., Lakhani, M., Patel, V., Shah, L., Vyas, V., & Shah, M. B. (2025) · Pharmacognosy Research · doi:10.5530/pres.20252199 · Cited by 1

Method Development for Quantitative Determination of Diosgenin from the Seeds of Fenugreek, Trigonella foenum-graecum L.

Sukhanov, A. E., Ставрианиди, А. Н., Кубасова, Е. Д., Panasyuk, A. S., & Buyuklinskaya, O. V. (2020) · Drug development & registration · doi:10.33380/2305-2066-2020-9-3-150-156