TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
Open accessAbstract
TBX3 is a member of the T‐box transcription factor family and is involved in the core pluripotency network. Despite this role in the pluripotency network, its contribution to the reprogramming process during the generation of human induced pluripotent stem cells remains elusive. In this respect, we performed reprogramming experiments applying TBX3 knockdown in human fibroblasts and keratinocytes. Knockdown of TBX3 in both somatic cell types decreased the reprogramming efficiencies in comparison to control cells but with unchanged reprogramming kinetics. The resulting iPSCs were indistinguishable from control cells and displayed a normal in vitro differentiation capacity by generating cells of all three germ layers comparable to the controls.
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TBX3 is critically involved in pluripotency maintenance and lineage-specific exit from the pluripotency circuitry in mouse embryonic stem cells.
“Finally, TBX3 is not only critically involved in pluripotency maintenance but also in lineage-specific exit from the pluripotency circuitry in mouse embryonic stem cells [11–17].”
From TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration (2023) · cites this paper as [12]
The Introduction reports that TBX3 helps maintain the stem-cell state and that its depletion leads to differentiation of murine pluripotent stem cells. It also describes roles in mesendodermal and endodermal differentiation, but does not explicitly establish lineage-specific exit from pluripotency in mouse embryonic stem cells. The paper’s own experiments concern human cells. (LLM, conf=0.78)
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Self-citationTBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration
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“Thus, we employed an inducible knockdown of TBX3 by an shRNA in a previously reported iPSC line [12] during in vitro pancreatic progenitor differentiation [58–61].”
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Self-citationMelzer, M. K., Schirge, S., Gout, J., Arnold, F., Srinivasan, D., Burtscher, I., Allgöwer, C., Mulaw, M., Zengerling, F., Güneş, Ç., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26576156
Self-citationMelzer, M. K., Schirge, S., Gout, J., Arnold, F., Srinivasan, D., Burtscher, I., Allgöwer, C., Mulaw, M., Zengerling, F., Güneş, Ç., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26576162
Self-citationMelzer, M. K., Schirge, S., Gout, J., Arnold, F., Srinivasan, D., Burtscher, I., Allgöwer, C., Mulaw, M., Zengerling, F., Güneş, Ç., et al. (2024) · Figshare · doi:10.6084/m9.figshare.26576159
Self-citationLiao, Y., Yang, J., Kuo, H., Chen, Y., Lin, Y., Sachdev, S., Kim, D., Roberts, R. M., Yuan, Y., & Ezashi, T. (2026) · FEBS Journal · doi:10.1111/febs.70733
- Authors
- Klingenstein, M., Raab, S., Achberger, K., Kleger, A., Liebau, S., & Linta, L.
- Affiliations
- University of Tübingen (DE)
- Universität Ulm (DE)
- Corresponding author
- Kleger, Alexander
- Liebau, Stefan
- Linta, Leonhard
- ORCID
- Venue
- Stem Cells International
- Publisher
- Wiley
- Published
- 2015-11-30
- Volume
- 2016
- Issue
- 1
- Pages
- 6759343-6759343
- ISSN
- 1687-966X (ISSN-L)
- 1687-9678
- PMC
- PMC4677243
- OpenAlex
- W2233224688
- Cited by
- 11
- References
- 20
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 4
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cells International (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Deutsche Forschungsgemeinschaft (BO1718/4-1, 2011_A200, 2248-0-0)
- Forschungskern SyStaR (BO1718/4-1, 2011_A200, 2248-0-0)
- Böhringer Ingelheim Ulm (BO1718/4-1, 2011_A200, 2248-0-0)
- Else-Kröner-Fresenius-Stiftung (BO1718/4-1, 2011_A200, 2248-0-0)
- Baden-Württemberg Stiftung (BO1718/4-1, 2011_A200, 2248-0-0)
- Faculty of Medicine Tübingen (BO1718/4-1, 2011_A200, 2248-0-0)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- TBX3, induced pluripotent stem cells, somatic cell reprogramming, T-box transcription factor, pluripotency network, fibroblasts, keratinocytes, iPSC differentiation, gene knockdown
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