Cited by 227Probiotics and Fermented FoodsImmune Response and InflammationReproductive tract infections research

Probiotic Lactobacillus Strains Stimulate the Inflammatory Response and Activate Human Macrophages

Open access
Rocha-Ramírez, L. M., Pérez-Solano, R. A., Castañón-Alonso, S. L., Moreno Guerrero, S. S., Ramírez Pacheco, A., García Garibay, M., Eslava, C.·2017·Journal of Immunology Research

Abstract

Lactobacilli have been shown to promote health functions. In this study, we analyzed the mechanism by which four different strains of probiotics affected innate immunity, such as regulation of ROS, cytokines, phagocytosis, bactericidal activity, signaling by NF- κ B pp65, and TLR2 activation. The production of ROS was dependent on the concentration and species of Lactobacillus . The results obtained from the tested strains ( Lactobacillus rhamnosus GG, L. rhamnosus KLSD, L. helveticus IMAU70129, and L. casei IMAU60214) showed that strains induced early proinflammatory cytokines such as IL-8,TNF- α , IL-12p70, and IL-6. However, IL-1 β expression was induced only by L. helveticus and L. casei strains (after 24 h stimulation). Phagocytosis and bactericidal activity of macrophages against various pathogens, such as S. aureus , S. typhimurium , and E. coli , were increased by pretreatment with Lactobacillus . The nuclear translocation NF- κ B pp65 and TLR2-dependent signaling were also increased by treatment with the probiotics. Taken together, the experiments demonstrate that probiotic strains of Lactobacillus exert early immunostimulatory effects that may be directly linked to the initial inflammation of the response of human macrophages.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1155/2017/4607491

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. [12]Zhang Y. Zhang L. Du M. Antimicrobial activity against Shigellasonnei and probiotic properties of wild lactobacillus from fermented food Microbiological Research 2011 167 1 27 32 21466951

Cited by

Sorted by citations

Authors
Rocha-Ramírez, L. M., Pérez-Solano, R. A., Castañón-Alonso, S. L., Moreno Guerrero, S. S., Ramírez Pacheco, A., García Garibay, M., & Eslava, C.
Affiliations
  • Hospital Infantil de México Federico Gómez (MX)
  • Departamento de Hemato-Oncología, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, 06720 Delegación Cuauhtémoc, MEX, Mexico
  • Departamento de Biotecnología, Unidad Iztapalapa and Departamento de Ciencias de la Alimentación, Unidad Lerma, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco No. 186, Col Vicentina, 09340 Mexico City, Mexico
Corresponding author
Rocha-Ramírez, L. M.
Venue
Journal of Immunology Research
Publisher
Wiley
Published
2017
Volume
2017
Pages
1-14
ISSN
  • 2314-7156 (ISSN-L)
  • 2314-8861
OpenAlex
W2729267312
Cited by
227
References
43
Citations by year
2026: 14 · 2025: 23 · 2024: 25 · 2023: 47 · 2022: 29 · 2021: 42 · 2020: 25 · 2019: 9 · 2018: 10 · 2017: 1
FWCI
13.85
Type
journal article
Language
en
Open access
Locations
  • Journal of Immunology Research (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Fondos Federales México (HIM/2011/007 SSA.929, HIM/2014/013 SSA.1120)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Keywords
probiotics, Lactobacillus, macrophages, innate immunity, inflammation, reactive oxygen species, inflammatory cytokines, phagocytosis, bactericidal activity, NF-κB signaling, TLR2, Lactobacillus rhamnosus, Lactobacillus helveticus, Lactobacillus casei, Lactobacillus rhamnosus GG, Staphylococcus aureus, Salmonella typhimurium, Escherichia coli
MeSH terms
Cells, Cultured, Humans, Immunity, Innate, Inflammation, Lactobacillus, Macrophage Activation, Phagocytosis, Tumor Necrosis Factor-alpha, Signal Transduction, Interleukin-6, Cytokines, Interleukin-8, NF-kappa B, Interleukin-12, Probiotics, Lacticaseibacillus rhamnosus

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review