Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria
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Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains,Lactococcus lactisSLT10,Lactobacillus plantarumC7, andLeuconostoc mesenteroidesB3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane. EPS recovery by ultrafiltration was higher than ethanol precipitation forLactococcus lactisSLT10 andLactobacillus plantarumC7. However, it was similar with both methods forLeuconostoc mesenteroidesB3. The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods. EPS isolated fromLactococcus lactisSLT10 are composed of glucose and mannose for EPS-ETOH against glucose, mannose, and rhamnose for EPS-UF. EPS extracted fromLactobacillus plantarumC7 andLeuconostoc mesenteroidesB3 showed similar composition (glucose and mannose) but different molar ratios. The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa. Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions. Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
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Membrane filtration techniques such as microfiltration, ultrafiltration and/or diafiltration have been used as a preceding step to EPS purification.
“As a preceding step to EPS purification, membrane filtration techniques such as microfiltration, ultrafiltration and/or diafiltration have also been used [50,58,70,101].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [50]
The Introduction lists microfiltration, ultrafiltration and diafiltration as procedures used for EPS purification. The Methods describe filtration before the purification step, but use ultrafiltration as a purification method itself; the paper therefore does not establish that all three techniques are used as preceding steps. (LLM, conf=0.85)
Experimental — accuracy not yet measuredEthanol is commonly used for precipitation of EPS, although isopropanol, acetone or a mixture of acetone and ethanol have also been used.
“For the following precipitation of EPS, ethanol is commonly used [27,42,43,50,55,58,99], although isopropanol [100], acetone [33] or a mixture of acetone and ethanol [101] have been also used.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [50]
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269 · see citing works
Raman and infrared spectroscopy of carbohydrates: A review
Wiercigroch, E., Szafraniec, E., Czamara, K., Pacia, M. Z., Majzner, K., Kochan, K., Kaczor, A., Barańśka, M., & Małek, K. (2017) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2017.05.045 · Cited by 1,078 · see citing works
Xu, F., Yu, J., Tesso, T., Dowell, F. E., & Wang, D. (2013) · Applied Energy · doi:10.1016/j.apenergy.2012.12.019 · Cited by 987 · see citing works
An overview of the functionality of exopolysaccharides produced by lactic acid bacteria
Ruas‐Madiedo, P., Hugenholtz, J., & Zoon, P. (2002) · International Dairy Journal · doi:10.1016/s0958-6946(01)00160-1 · Cited by 625 · see citing works
Exopolysaccharides from lactic acid bacteria: perspectives and challenges
Welman, A. D., & Maddox, I. S. (2003) · Trends in biotechnology · doi:10.1016/s0167-7799(03)00107-0 · Cited by 523 · see citing works
New Perspectives for Lactobacilli Exopolysaccharides
Badel, S., Bernardi, T., & Michaud, P. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.08.011 · Cited by 497 · see citing works
Exocellular polysaccharides produced by lactic acid bacteria
Cerning, J. (1990) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1990.tb04883.x · Cited by 470 · see citing works
Ruas-Madiedo, P., & de los Reyes-Gavilán, C. G. (2005) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(05)72750-8 · Cited by 456 · see citing works
Comte, S., Guibaud, G., & Baudu, M. (2005) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2005.06.016 · Cited by 444 · see citing works
Wang, Y., Li, C., Liu, P., Ahmed, Z., Xiao, P., & Bai, X. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.06.013 · Cited by 407 · see citing works
De Vuyst, L., De Vin, F., Vaningelgem, F., & Degeest, B. (2001) · International Dairy Journal · doi:10.1016/s0958-6946(01)00114-5 · Cited by 391 · see citing works
Antioxidant Activity of an Exopolysaccharide Isolated from Lactobacillus plantarum C88
Zhang, L., Liu, C., Li, D., Zhao, Y., Zhang, X., Zeng, X., Yang, Z., & Li, S. (2013) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2012.12.037 · Cited by 371 · see citing works
Kamerling, J. P., Gerwig, G. J., Vliegenthart, J. F. G., & Clamp, J. R. (1975) · Biochemical Journal · doi:10.1042/bj1510491 · Cited by 353 · see citing works
Wang, J., Zhao, X. Y., Tian, Z., Yang, Y., & Yang, Z. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.03.003 · Cited by 340 · see citing works
Melo, M. R. S. (2002) · Carbohydrate Polymers · doi:10.1016/s0144-8617(02)00006-1 · Cited by 315 · see citing works
Biosynthesis, characterisation, and design of bacterial exopolysaccharides from lactic acid bacteria
Laws, A. P., Gu, Y., & Marshall, V. M. (2001) · Biotechnology Advances · doi:10.1016/s0734-9750(01)00084-2 · Cited by 279 · see citing works
van Kranenburg, R., Marugg, J. D., van Swam, I. I., Willem, N. J., & de Vos, W. M. (1997) · Molecular Microbiology · doi:10.1046/j.1365-2958.1997.3521720.x · Cited by 262 · see citing works
Isolation and Characterization of Two Exopolysaccharides Produced by Lactobacillus plantarum EP56
Tallon, R., Bressollier, P., & Urdaci, M. C. (2003) · Research in Microbiology · doi:10.1016/j.resmic.2003.09.006 · Cited by 260 · see citing works
Zeng, W., Zhang, Z., Gao, H. X., Jia, L., & Chen, W. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.03.078 · Cited by 222 · see citing works
Li, C., Li, W., Chen, X., Feng, M., Rui, X., Jiang, M., & Dong, M. (2014) · LWT - Food Science and Technology · doi:10.1016/j.lwt.2014.02.025 · Cited by 219 · see citing works
Pham, P. L., Dupont, I., Roy, D., Lapointe, G., & Cerning, J. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2302-2310.2000 · Cited by 212 · see citing works
Antioxidant Activity of an Exopolysaccharide Purified from Lactococcus lactis subsp. lactis 12
Pan, D., & Mei, X. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.01.005 · Cited by 211 · see citing works
Liu, C., Lü, J., Lu, L., Liu, Y., Wang, F., & Xiao, M. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.01.151 · Cited by 211 · see citing works
Biosorption of Cu2+, Pb2+ and Cr6+ by a novel exopolysaccharide from Arthrobacter ps-5
Ye, S., Zhang, M., Hong, Y. H., Han, W., Xiao, S., Liu, Y., & Jihui, W. (2013) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2013.09.021 · Cited by 182 · see citing works
Characteristics and turnover of exopolymeric substances in a hypersaline microbial mat
Braissant, O., Decho, A. W., Przekop, K. M., Gallagher, K. L., Glunk, C., Dupraz, C., & Visscher, P. T. (2008) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2008.00614.x · Cited by 176 · see citing works
van Geel-Schutten, G. H., Faber, E. J., Smit, E., Bonting, K., Smith, M. R., ten Brink, B., Kamerling, J. P., Vliegenthart, J. F. G., & Dijkhuizen, L. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.7.3008-3014.1999 · Cited by 176 · see citing works
Gallaher, W. R., DiSimone, C., & Buchmeier, M. J. (2001) · BMC Microbiology · doi:10.1186/1471-2180-1-1 · Cited by 172 · see citing works
Chen, W., Wang, W., Zhang, H., & Huang, Q. (2011) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.08.029 · Cited by 171 · see citing works
Degeest, B., & Vuyst, L. D. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.7.2863-2870.1999 · Cited by 148 · see citing works
Antioxidant activities of an exopolysaccharide isolated and purified from marine Pseudomonas PF-6
Ye, S., Liu, F., Wang, J., Wang, H., & Zhang, M. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.08.057 · Cited by 138 · see citing works
Domínguez, L., Pastor, M. R., & Prats, D. (2010) · Desalination · doi:10.1016/j.desal.2010.05.054 · Cited by 137 · see citing works
Nakajima, H., Hirota, T., Toba, T., Itoh, T., & Adachi, S. (1992) · Carbohydrate Research · doi:10.1016/0008-6215(92)84110-e · Cited by 120 · see citing works
Analysis of the native structure of starch granules with small angle x-ray microfocus scattering
Waigh, T. A., Donald, A. M., Heidelbach, F., Riekel, C., & Gidley, M. J. (1999) · Biopolymers · doi:10.1002/(sici)1097-0282(199901)49:1 · Cited by 116 · see citing works
A new exopolysaccharide produced by marine Cyanothece sp. 113
Chi, Z., Su, C., & Lu, W. (2006) · Bioresource Technology · doi:10.1016/j.biortech.2006.05.001 · Cited by 112 · see citing works
Hanko, V. P., & Rohrer, J. S. (2000) · Analytical Biochemistry · doi:10.1006/abio.2000.4653 · Cited by 104 · see citing works
Grobben, G. J., van Casteren, W. H. M., Schols, H. A., Oosterveld, A., Sala, G., Smith, M. R., Sikkema, J., & de Bont, J. A. M. (1997) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051089 · Cited by 103 · see citing works
Analysis and production of two exopolysaccharides fromLactococcus lactissubsp.cremorisLC330
Marshall, V. M., Cowie, E. N., & Moreton, R. S. (1995) · Journal of Dairy Research · doi:10.1017/s0022029900031356 · Cited by 97 · see citing works
Structural analysis of the exopolysaccharides produced by Lactobacillus spp. G-77
Dueñas, M. T., Rodríguez-Carvajal, M. Á., Tejero‐Mateo, P., Espartero, J. L., Irastorza, A., & Serrano, A. M. G. (1998) · Carbohydrate Research · doi:10.1016/s0008-6215(98)00034-2 · Cited by 88 · see citing works
Looijesteijn, P. J., & Hugenholtz, J. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80198-4 · Cited by 87 · see citing works
van Kranenburg, R., van Swam, I. I., Marugg, J. D., Kleerebezem, M., & de Vos, W. M. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.1.338-340.1999 · Cited by 86 · see citing works
Guo, Y., Pan, D., Sun, Y., Xin, L., Li, H., & Zeng, X. (2013) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2013.06.024 · Cited by 83 · see citing works
Purification and characterization of a novel exopolysaccharides produced by Lactobacillus sp. Ca6
Trabelsi, I., Slima, S. B., Chaabane, H., & Salah, R. B. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2014.12.045 · Cited by 82 · see citing works
Yang, Z., Huttunen, E., Staaf, M., Widmalm, G., & Tenhu, H. J. (1999) · International Dairy Journal · doi:10.1016/s0958-6946(99)00133-8 · Cited by 81 · see citing works
Growth Associated Exopolysaccharide Expression in Lactococcus lactis subspecies cremoris Ropy352
Knoshaug, E. P., Ahlgren, J. A., & Trempy, J. E. (2000) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(00)74923-x · Cited by 79 · see citing works
d’Abzac, P., Bordas, F., Joussein, E., van Hullebusch, E. D., Lens, P. N. L., & Guibaud, G. (2012) · Environmental Science and Pollution Research · doi:10.1007/s11356-012-1401-3 · Cited by 68 · see citing works
van Casteren, W. H. M., Dijkema, C., Schols, H. A., BELDMAN, G., & Voragen, A. G. J. (1998) · Carbohydrate Polymers · doi:10.1016/s0144-8617(98)00044-7 · Cited by 67 · see citing works
Vijayendra, S. V. N., Palanivel, G., Mahadevamma, S., & Tharanathan, R. N. (2007) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2007.08.016 · Cited by 61 · see citing works
Marimuthu, P., Aldars‐García, L., Gil‐Ramírez, A., Ruiz‐Rodríguez, A., Marín, F. R., Reglero, G., & Soler‐Rivas, C. (2014) · Biotechnology Progress · doi:10.1002/btpr.1865 · Cited by 57 · see citing works
Preparative fractionation of polysaccharides by columns packed with regenerated cellulose gels
Zhang, L., Zhou, J., Yang, G., & Chen, J. (1998) · Journal of Chromatography A · doi:10.1016/s0021-9673(98)00475-0 · Cited by 56 · see citing works
Kenney, J. P. L., & Fein, J. B. (2011) · Chemical Geology · doi:10.1016/j.chemgeo.2011.04.011 · Cited by 54 · see citing works
New method for exopolysaccharide determination in culture broth using stirred ultrafiltration cells
Bergmaier, D., Lacroix, C., Macedo, M. G., & Champagne, C. P. (2001) · Applied Microbiology and Biotechnology · doi:10.1007/s002530100764 · Cited by 53 · see citing works
Bourven, I., Joussein, E., & Guibaud, G. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.04.058 · Cited by 50 · see citing works
Forde, A., & Fitzgerald, G. F. (2003) · Plasmid · doi:10.1016/s0147-619x(02)00156-7 · Cited by 46 · see citing works
Higashimura, M., Mulder-Bosman, B. W., Reich, R., Iwasaki, T., & Robijn, G. W. (2000) · Biopolymers · doi:10.1002/1097-0282(200008)54:2 · Cited by 36 · see citing works
Maâlej, H., Boisset, C., Hmidet, N., Buon, L., Heyraud, A., & Nasri, M. (2014) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2014.06.003 · Cited by 34 · see citing works
Levander, F., Svensson, M., & Rådström, P. (2001) · BMC Microbiology · doi:10.1186/1471-2180-1-23 · Cited by 32 · see citing works
The effect of heat stress on freeze-drying and conservation of Lactococcus
Ziadi, M., Touhami, Y., Achour, M., Thonart, P., & Hamdi, M. (2005) · Biochemical Engineering Journal · doi:10.1016/j.bej.2005.02.001 · Cited by 32 · see citing works
Self-citationMicrobiological analysis and screening of lactic acid bacteria from Tunisian date palm sap
Ziadi, M., hir, S. M., Kbaier, N., Hamdi, M., & Ferchichi, A. (2011) · African Journal of Microbiology Research · doi:10.5897/ajmr11.325 · Cited by 30 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 3
- [13]Marshall V. M. Cowie E. N. Moreton R. S. Analysis and Production of 2 Exopolysaccharides from Lactococcus-Lactis subsp. cremoris Lc330 Journal of Dairy Research 1995 62 621 628
- [44]Montreuil J. Bouquelet S. Debray H. Fournet B. Spik G. Strecker G. Chaplin M. F. Kennedy J. F. Glycoproteins Carbohydrate Analysis: A Practical Approach 1986 New York, NY, USA Oxford University press 143 204
- [50]Cote GL. Ahlgren J. Kirk-Othmer A. Microbial polysaccharides, in Encyclopedia of chemical technology 1995 4th New York, NY, USA John Wiley and Sons Inc
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Mora-Villalobos, J.-A., Montero‐Zamora, J., Barboza, N., Rojas-Garbanzo, C., Usaga, J., Redondo-Solano, M., Schroedter, L., Olszewska‐Widdrat, A., & López‐Gómez, J. P. (2020) · Fermentation · doi:10.3390/fermentation6010023 · Cited by 204 · see citing works
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194 · see citing works
“lactis SLT10 280–336 Modified MRS Sucrose Glucose, Mannose, Rhamnose [50]”
MethodInfluentialTiwari, S., Kavitake, D., Devi, P. B., & Shetty, P. H. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.05.140 · Cited by 159 · see citing works
Wu, J., Han, X., Ye, M., Li, Y., Wang, X., & Zhong, Q. (2022) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2022.2043822 · Cited by 106 · see citing works
Yadav, M. K., Song, J. H., Vasquez, R., Lee, J. S., Kim, I. H., & Kang, D. (2024) · Foods · doi:10.3390/foods13223687 · Cited by 87 · see citing works
Al Disi, Z. A., Zouari, N., Dittrich, M., Jaoua, S., Al-Kuwari, H. A. S., & Bontognali, T. R. R. (2019) · Marine Chemistry · doi:10.1016/j.marchem.2019.103693 · Cited by 61 · see citing works
Fuso, A., Bancalari, E., Castellone, V., Caligiani, A., Gatti, M., & Bottari, B. (2023) · Foods · doi:10.3390/foods12010215 · Cited by 57 · see citing works
Elmansy, E. A., El-Kady, E. M., Asker, M. S., Abdou, A. M., Abdallah, N. A., & Amer, S. K. (2022) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-022-03439-6 · Cited by 50 · see citing works
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Wang, L., Gu, Y., Zheng, X., Zhang, Y., Deng, K., Wu, T., & Cheng, H. S. (2021) · LWT · doi:10.1016/j.lwt.2021.111449 · Cited by 44 · see citing works
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Jha, Y., Yadav, K. A., & Mohamed, H. I. (2023) · Plant and Soil · doi:10.1007/s11104-023-06155-8 · Cited by 26 · see citing works
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Wang, B., Sun, X., Xu, M., Wang, F., Liu, W., & Wu, B. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1108120 · Cited by 18 · see citing works
Pandey, S., & Kannaujiya, V. K. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.135261 · Cited by 18 · see citing works
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Liu, Y., Zhou, Y. C., Bian, C., Li, H., Kang, Y., Gao, Y. W., Yao, P., & Zhang, C. (2024) · Antioxidants · doi:10.3390/antiox13111289 · Cited by 14 · see citing works
Zammouri, A., Ziadi, M., Gharsallaoui, A., Fguiri, I., Sbissi, I., Hammadi, M., & Khorchani, T. (2024) · Fermentation · doi:10.3390/fermentation10100532 · Cited by 14 · see citing works
Self-citationYao, X., Chen, P., Cheng, T., Sun, K., Megharaj, M., & He, W. (2021) · Journal of Applied Microbiology · doi:10.1111/jam.14983 · Cited by 14 · see citing works
Manivannan, N., Babu, S. P. S., Malaikozhundan, B., Ramachandran, R., & Johney, J. (2021) · Bioactive Carbohydrates and Dietary Fibre · doi:10.1016/j.bcdf.2021.100297 · Cited by 13 · see citing works
Abdel-monem, D. A., Sabry, S. A., Ghozlan, H. A., & Zaghloul, E. H. (2024) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-024-10334-z · Cited by 12 · see citing works
Youssif, A. M., Hamed, M. M., & Abdrabo, M. A. A. (2020) · Journal of Pure and Applied Microbiology · doi:10.22207/jpam.14.4.51 · Cited by 12 · see citing works
Pointcheval, M., Massé, A., Floc’hlay, D., Chanonat, F., Estival, J., & Durand, M.-J. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1536185 · Cited by 10 · see citing works
Kinoshita, H., Hariu, M., Nakashima, Y., Watanabe, K., Yasuda, S., & Igoshi, K. (2020) · Microbiology · doi:10.1099/mic.0.000936 · Cited by 9 · see citing works
Mao, Y., Wang, W., Mo, W., Yang, B., Han, Y., Guo, Y., & Li, S. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.137008 · Cited by 7 · see citing works
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Bashandy, S. R., Abd‐Alla, M. H., Mohammed, E. A., & Hassan, E. A. (2024) · Current Microbiology · doi:10.1007/s00284-024-03913-4 · Cited by 7 · see citing works
Besednova, N. N., Andryukov, B. G., Kuznetsova, T. A., Zaporozhets, T. S., Kryzhanovsky, S. P., Ermakova, S. P., & Shchelkanov, M. Y. (2022) · Journal of Pharmacy and Nutrition Sciences · doi:10.29169/1927-5951.2022.12.05 · Cited by 7 · see citing works
Bunkaew, K., Yenraksa, A., Pewhom, A., Kiriratnikom, S., Sewaka, M., Lertworapreecha, M., & Chanasit, W. (2024) · Microbiology Research · doi:10.3390/microbiolres15040148 · Cited by 6 · see citing works
Antioxidant activity of exopolysaccharides from marine cyanobacteria
Hazra, P., Ghosh, S., Roy, P., Sinha, D., & Kesh, G. S. (2025) · The Microbe · doi:10.1016/j.microb.2025.100430 · Cited by 6 · see citing works
Ting, A. S. Y., Tiew, K. H., & Song, K. P. (2024) · International Journal of Environmental Science and Technology · doi:10.1007/s13762-024-06224-0 · Cited by 6 · see citing works
Integrating waste management with biopolymer innovation: A new frontier in xanthan gum production
Asase, R. V., & Glukhareva, T. V. (2025) · Process Biochemistry · doi:10.1016/j.procbio.2025.04.012 · Cited by 6 · see citing works
Jungcharoen, P., Mekhin, P., Seelaphat, J., Thongbai, P., & Ekprasert, J. (2024) · Water Environment Research · doi:10.1002/wer.11102 · Cited by 5 · see citing works
Murugesan, H., Shobanan, S., Tamilmani, K. K., Sushmitha, T. J., Balachandran, K. R. S., Peter, D. M., Rangamaran, V. R., & Gopal, D. (2025) · Microbiology · doi:10.1134/s0026261724610297 · Cited by 5 · see citing works
Sulfated Polysaccharide from Marine Red Microalga Porphyridium against SARS-CoV-2 - A Mini-Review
Gaikwad, M., Pawar, Y., Gangal, N., Nagle, V. L., & Dasgupta, S. (2022) · ACTA SCIENTIFIC MICROBIOLOGY · doi:10.31080/asmi.2022.05.1012 · Cited by 5 · see citing works
Hamdaoui, N., Sabbahi, R., Azzaoui, K., Meziane, M., & Hammouti, B. (2025) · International Microbiology · doi:10.1007/s10123-025-00745-5 · Cited by 4 · see citing works
Extracellular Polymeric Substances in Textile Industry
Topal, M., & Topal, E. I. A. (2022) · Sustainable textiles · doi:10.1007/978-981-19-0878-1_2 · Cited by 4 · see citing works
Zhang, J., Hu, T., & Sun, Q. (2024) · Food Science and Human Wellness · doi:10.26599/fshw.2024.9250368 · Cited by 3 · see citing works
Lozano, A., González-Galán, A. M., López‐Linares, J. C., Coca, M., Cubero, M. T. G., & Lucas, S. (2026) · Bioresource Technology Reports · doi:10.1016/j.biteb.2026.102638 · Cited by 3 · see citing works
Ting, A. S. Y., Tiew, K. H., & Song, K. P. (2026) · Biodegradation · doi:10.1007/s10532-026-10280-3 · Cited by 3 · see citing works
Devadig, R., Handa, V., Jawaharraj, K., Talluri, S. N. L., Dhiman, S. S., Gu, T., & Gadhamshetty, V. R. (2025) · Corrosion Science · doi:10.1016/j.corsci.2025.112988 · Cited by 3 · see citing works
Narayana, P. V. S. L. S. S., & Dutta, J. R. (2019) · Current Microbiology · doi:10.1007/s00284-019-01780-y · Cited by 3 · see citing works
Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors
Mielnichuk, N., Joya, C. M., Monachesi, M. A., & Bertani, R. P. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-3617-6_5 · Cited by 3 · see citing works
Lisboa, G. H., Pena, A. D. C. C., Rodrigues, A., & Rodrigues, S. Á. (2025) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2025.2471890 · Cited by 2 · see citing works
Muhardina, V., Uttapap, D., Wandee, Y., Kotatha, D., Udchumpisai, W., & Rungsardthong, V. (2023) · Waste and Biomass Valorization · doi:10.1007/s12649-023-02131-6 · Cited by 2 · see citing works
Zennathara, Afrin, S., Ali, M. A., Islam, M. N., & Saha, M. L. (2023) · Dhaka University Journal of Biological Sciences · doi:10.3329/dujbs.v32i2.67683 · Cited by 2 · see citing works
Tounsi, L., Hlima, H. B., Soltana, O. B., Duchez, D., Cadre, E. L., Gardarin, C., Fendri, I., Michaud, P., & Abdelkafi, S. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.146021 · Cited by 2 · see citing works
Singh, A., Ahmed, M., Iqbal, U., Srivastava, U., & Saini, P. (2024) · Food Safety and Health · doi:10.1002/fsh3.12065 · Cited by 1 · see citing works
Methods of Extraction of Beta-Glucan
Sargar, S., Tiwari, S., Reddy, G. B., & Kavitake, D. (2025) · Springer eBooks · doi:10.1007/978-3-031-95788-8_3 · Cited by 1 · see citing works
Gomri, M. A., Bachtarzi, N., Boulahlib, C. Y., Bisson, G., Comuzzi, C., Marino, M., Benmoussa, A., & Kharroub, K. (2025) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-025-10765-2 · Cited by 1 · see citing works
Zhao, H., Zhang, Z., Chen, X., Tang, C., Song, L., Zhaoxin, L., & Lu, Y. (2026) · Foods · doi:10.3390/foods15030409 · Cited by 1 · see citing works
Riyamol, Mittal, A., Ahmad, F., Haque, S., Chengaiyan, J. G., & Yadav, D. K. (2026) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2026.152873 · Cited by 1 · see citing works
Ss, V., V, A., Desai, G. B., Pawase, P., Krishnan, H., Bashir, O., & Abdi, G. (2026) · Food Chemistry Advances · doi:10.1016/j.focha.2026.101369 · Cited by 1 · see citing works
Kabir, A., Shamsuddeen, U., & Magashi, A. M. (2022) · UMYU Journal of Microbiology Research (UJMR) · doi:10.47430/ujmr.2271.014 · Cited by 1 · see citing works
Khan, M., Imran, M., Ashraf, M., Ishaque, W., & Habib, M. (2024) · Current Microbiology · doi:10.1007/s00284-023-03607-3 · Cited by 1 · see citing works
Freitas, G. M. D. O., da Silva, F. D. P. S., & Matta, A. E. R. (2019) · Cadernos de Ciências Sociais Aplicadas · doi:10.22481/ccsa.v16i28.5854 · Cited by 1 · see citing works
Demir, A. T., Çadırcı, B. H., & Altun, A. (2022) · Cumhuriyet Tıp Dergisi/Cumhuriyet Üniversitesi Tıp Fakültesi dergisi · doi:10.7197/cmj.1156620
The Ability of Lactobacillus Plantarum to Produce Exopolysaccharides with Additional Nutrients
Puspadewi, R., Gumilar, A., & Kartikasari, T. D. (2022) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/1104/1/012011
Khan, M., Imran, M., Ashraf, M., Ishaque, W., & Habib, M. (2023) · Research Square · doi:10.21203/rs.3.rs-3254101/v1
ACCUMULATION OF EXOPOLYSACCHARIDES BY YEASTS OF RHODOTORULA SP.
Cheban, L. M., & Vasina, L. (2023) · Biolohichni systemy · doi:10.31861/biosystems2023.01.013
Пожидаева, Е., Попов, Е. С., Родионова, Н. С., Dymovskikh, Y. A., Durova, Y. V., & Grebennikova, M. (2024) · BIO Web of Conferences · doi:10.1051/bioconf/202412601044
Herpandi, Saputra, E. P., Nugroho, G. D., Widiastuti, I., Janna, M., & Sudirman, S. (2025) · Tropical Journal of Natural Product Research · doi:10.26538/tjnpr/v9i2.26
Choi, J., Lee, S., Kim, D.-H., Han, Y., Rhyu, H., Lee, J. H. J., & Han, S. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1556578
Extraction Techniques: Past, Present, and Future
Mazumder, S., Bhattacharya, D., Lahiri, D., & Nag, M. (2025) · Springer eBooks · doi:10.1007/978-3-031-94472-7_3
Yu, X., Liu, J., Zhang, J., Li, A., Li, C., Zhang, G., Du, P., & Liu, L. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c10046
Kim, H., Oh, N., Kwon, M., Kwon, O.-H., Ku, S., Seo, J., & Roh, S. (2022) · Figshare · doi:10.6084/m9.figshare.20887275
Wang, Q., Zhao, W., Wu, Y., Liao, Y., Zhou, W., Xie, D., & Jin, Y. (2026) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2026.152203
Biopolymer sources: extraction and purification approaches
Medimagh, M., Khiari, R., Garcı́a, A., Moussaoui, Y., & Belgacem, M. N. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-33393-4.00005-0
Lee, Y.-N., Song, S. M., Charchoghlyan, H., Choi, J.-S., Kim, D. H., Choe, D., Jung, Y. H., & Lee, S.-B. (2026) · LWT · doi:10.1016/j.lwt.2026.119544
Solis-Arevalo, K. K., L.J., L. J. G.-W., Rodríguez‐García, A., & Arévalo‐Niño, K. (2026) · Polymers · doi:10.3390/polym18131679
Charlet, R., Lecointe, K., plu, O., Cordier, C., Bortolus, C., Popczyk, P., Dubar, F., Grec, S., & Sendid, B. (2026) · Virulence · doi:10.1080/21505594.2026.2707757
Khadraoui, I., & Gomri, M. A. (2026) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-026-04941-3
Lee, H., Chae, Y.-R., Lee, Y. R., Kim, Y., & Park, H. (2026) · Food Science & Nutrition · doi:10.1002/fsn3.72395
dos Santos, N. P., Baptista, R. M., Kubelka, B. G., D’Oca, M. G. M., de Lima, J. T., Bueno, D. T., Leitzke, A. F., & Ramos, D. F. (2026) · Frontiers in Natural Products · doi:10.3389/fntpr.2026.1884549
- Authors
- Ziadi, M., Bouzaiene, T., M’Hir, S., Zaafouri, K., Mokhtar, F., Hamdi, M., & Boisset-Helbert, C.
- Affiliations
- University of Carthage (TN)
- Centre de Recherche sur les Macromolécules Végétales, CERMAV, CNRS, 601 rue de la Chimie, 38041 Grenoble Cedex 9, France
- Laboratory of Microbial Ecology and Technology, LETMi-INSAT, National Institute of Applied Sciences and Technology INSAT, Carthage University, 2 Boulevard de la Terre, BP 676, 1080 Tunis, Tunisia
- National Research Center for Materials Science, Borj-Cedria Technopark, BP N°73, 8027 Soliman, Tunisia
- Corresponding author
- Ziadi, Manel
- Venue
- BioMed Research International
- Publisher
- Wiley
- Published
- 2018-09-18
- Volume
- 2018
- Pages
- 1-11
- ISSN
- 1110-7243 (ISSN-L)
- 2314-6133
- 2314-6141
- PMC
- PMC6167595
- OpenAlex
- W2890723929
- Cited by
- 82
- References
- 59
- Citations by year
- 2026: 13 · 2025: 16 · 2024: 15 · 2023: 10 · 2022: 12 · 2021: 7 · 2020: 6 · 2019: 3
- FWCI
- 3.75
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- BioMed Research International (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- French Institute of Cooperation
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
- Keywords
- exopolysaccharides, lactic acid bacteria, ethanol precipitation, ultrafiltration, Lactococcus lactis, Lactobacillus plantarum, Leuconostoc mesenteroides, polysaccharide purification, monomer composition, molar ratio, molecular weight distribution, functional groups
- MeSH terms
- Leuconostoc mesenteroides, Ethanol, Polysaccharides, Bacterial, Lactococcus lactis, Ultrafiltration, Lactobacillus plantarum, Lactiplantibacillus plantarum
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