Cited by 82Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsBiopolymer Synthesis and Applications

Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria

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Ziadi, Manel, Bouzaiene, Taroub, M’Hir, Sana, Zaafouri, Kaouther, Mokhtar, Ferid, Hamdi, Mokhtar, Boisset-Helbert, Claire·2018·BioMed Research International

Abstract

Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains,Lactococcus lactisSLT10,Lactobacillus plantarumC7, andLeuconostoc mesenteroidesB3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane. EPS recovery by ultrafiltration was higher than ethanol precipitation forLactococcus lactisSLT10 andLactobacillus plantarumC7. However, it was similar with both methods forLeuconostoc mesenteroidesB3. The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods. EPS isolated fromLactococcus lactisSLT10 are composed of glucose and mannose for EPS-ETOH against glucose, mannose, and rhamnose for EPS-UF. EPS extracted fromLactobacillus plantarumC7 andLeuconostoc mesenteroidesB3 showed similar composition (glucose and mannose) but different molar ratios. The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa. Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions. Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.

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  • Membrane filtration techniques such as microfiltration, ultrafiltration and/or diafiltration have been used as a preceding step to EPS purification.

    “As a preceding step to EPS purification, membrane filtration techniques such as microfiltration, ultrafiltration and/or diafiltration have also been used [50,58,70,101].”

    From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [50]

    The Introduction lists microfiltration, ultrafiltration and diafiltration as procedures used for EPS purification. The Methods describe filtration before the purification step, but use ultrafiltration as a purification method itself; the paper therefore does not establish that all three techniques are used as preceding steps. (LLM, conf=0.85)

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  • Ethanol is commonly used for precipitation of EPS, although isopropanol, acetone or a mixture of acetone and ethanol have also been used.

    “For the following precipitation of EPS, ethanol is commonly used [27,42,43,50,55,58,99], although isopropanol [100], acetone [33] or a mixture of acetone and ethanol [101] have been also used.”

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Research works printed but not linked: 3

  1. [13]Marshall V. M. Cowie E. N. Moreton R. S. Analysis and Production of 2 Exopolysaccharides from Lactococcus-Lactis subsp. cremoris Lc330 Journal of Dairy Research 1995 62 621 628
  2. [44]Montreuil J. Bouquelet S. Debray H. Fournet B. Spik G. Strecker G. Chaplin M. F. Kennedy J. F. Glycoproteins Carbohydrate Analysis: A Practical Approach 1986 New York, NY, USA Oxford University press 143 204
  3. [50]Cote GL. Ahlgren J. Kirk-Othmer A. Microbial polysaccharides, in Encyclopedia of chemical technology 1995 4th New York, NY, USA John Wiley and Sons Inc

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Authors
Ziadi, M., Bouzaiene, T., M’Hir, S., Zaafouri, K., Mokhtar, F., Hamdi, M., & Boisset-Helbert, C.
Affiliations
  • University of Carthage (TN)
  • Centre de Recherche sur les Macromolécules Végétales, CERMAV, CNRS, 601 rue de la Chimie, 38041 Grenoble Cedex 9, France
  • Laboratory of Microbial Ecology and Technology, LETMi-INSAT, National Institute of Applied Sciences and Technology INSAT, Carthage University, 2 Boulevard de la Terre, BP 676, 1080 Tunis, Tunisia
  • National Research Center for Materials Science, Borj-Cedria Technopark, BP N°73, 8027 Soliman, Tunisia
Corresponding author
Ziadi, Manel
Venue
BioMed Research International
Publisher
Wiley
Published
2018-09-18
Volume
2018
Pages
1-11
ISSN
  • 1110-7243 (ISSN-L)
  • 2314-6133
  • 2314-6141
OpenAlex
W2890723929
Cited by
82
References
59
Citations by year
2026: 13 · 2025: 16 · 2024: 15 · 2023: 10 · 2022: 12 · 2021: 7 · 2020: 6 · 2019: 3
FWCI
3.75
Type
journal article
Language
en
Open access
Locations
  • BioMed Research International (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
French Institute of Cooperation
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
Keywords
exopolysaccharides, lactic acid bacteria, ethanol precipitation, ultrafiltration, Lactococcus lactis, Lactobacillus plantarum, Leuconostoc mesenteroides, polysaccharide purification, monomer composition, molar ratio, molecular weight distribution, functional groups
MeSH terms
Leuconostoc mesenteroides, Ethanol, Polysaccharides, Bacterial, Lactococcus lactis, Ultrafiltration, Lactobacillus plantarum, Lactiplantibacillus plantarum

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