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Cited by 417Immune cells in cancerNeutrophil, Myeloperoxidase and Oxidative MechanismsCancer Immunotherapy and Biomarkers

The DOI matches its Crossref record; 52 of the 53 references it cites were found in a registry or an index.

Tumor-Produced Interleukin-8 Attracts Human Myeloid-Derived Suppressor Cells and Elicits Extrusion of Neutrophil Extracellular Traps (NETs)

Alfaro, Carlos, Teijeira, Alvaro, Oñate, Carmen, Pérez, Guiomar, Sanmamed, Miguel F., Andueza, Maria Pilar, Alignani, Diego, Labiano, Sara, Azpilikueta, Arantza, Rodriguez-Paulete, Alfonso, Garasa, Saray, Fusco, Juan P., Aznar, Angela, Inogés, Susana, De Pizzol, Maria, Allegretti, Marcello, Medina-Echeverz, Jose, Berraondo, Pedro, Perez-Gracia, Jose L., Melero, Ignacio·2016·Clinical Cancer Research
DOI 10.1158/1078-0432.ccr-15-2463

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Abstract

Purpose: Myeloid-derived suppressor cells (MDSC) are considered an important T-cell immunosuppressive component in cancer-bearing hosts. The factors that attract these cells to the tumor microenvironment are poorly understood. IL8 (CXCL8) is a potent chemotactic factor for neutrophils and monocytes. Experimental Design: MDSC were characterized and sorted by multicolor flow cytometry on ficoll-gradient isolated blood leucokytes from healthy volunteers (n = 10) and advanced cancer patients (n = 28). In chemotaxis assays, sorted granulocytic and monocytic MDSC were tested in response to recombinant IL8, IL8 derived from cancer cell lines, and patient sera. Neutrophil extracellular traps (NETs) formation was assessed by confocal microscopy, fluorimetry, and time-lapse fluorescence confocal microscopy on short-term MDSC cultures. Results: IL8 chemoattracts both granulocytic (GrMDSC) and monocytic (MoMDSC) human MDSC. Monocytic but not granulocytic MDSC exerted a suppressor activity on the proliferation of autologous T cells isolated from the circulation of cancer patients. IL8 did not modify the T-cell suppressor activity of human MDSC. However, IL8 induced the formation of NETs in the GrMDSC subset. Conclusions: IL8 derived from tumors contributes to the chemotactic recruitment of MDSC and to their functional control. Clin Cancer Res; 22(15); 3924–36. ©2016 AACR.

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Metadata

Authors
Alfaro, C., Teijeira, A., Oñate, C., Pérez, G., Sanmamed, M. F., Andueza, M. P., Alignani, D., Labiano, S., Azpilikueta, A., Rodriguez-Paulete, A., Garasa, S., Fusco, J. P., Aznar, A., Inogés, S., De Pizzol, M., Allegretti, M., Medina-Echeverz, J., Berraondo, P., Perez-Gracia, J. L., & Melero, I.
Affiliations
  • Clinica Universidad de Navarra (ES)
  • 1Division of Gene Therapy and Hepatology, Centre for Applied Medical Research (CIMA), Pamplona, Spain.
  • Centro de Investigación Médica Aplicada (ES)
  • 5Dompé Farmaceutici SpA, L'Aquila, Italy.
  • 2Department of Oncology, University Clinic of Navarra, Pamplona, Spain.
ORCID
Venue
Clinical Cancer Research
Publisher
American Association for Cancer Research (AACR)
Published
2016-07-31
Volume
22
Issue
15
Pages
3924-3936
ISSN
  • 1078-0432 (ISSN-L)
  • 1557-3265
DOI
10.1158/1078-0432.ccr-15-2463
OpenAlex
W2298626553
Cited by
417
References
53
Citations by year
2026: 35 · 2025: 33 · 2024: 44 · 2023: 54 · 2022: 54 · 2021: 59 · 2020: 46 · 2019: 35 · 2018: 22 · 2017: 21 · 2016: 13
FWCI
18.36
Type
journal article
Language
en
Open access
closed
Locations
  • Clinical Cancer Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Health Sciences › Medicine › Oncology › Cancer Immunotherapy and Biomarkers
Keywords
interleukin-8, myeloid-derived suppressor cells, neutrophil extracellular traps, tumor microenvironment, chemotaxis, granulocytic MDSCs, monocytic MDSCs, T cell suppression, tumor immunosuppression, tumor-derived cytokines
MeSH terms
Myeloid-Derived Suppressor Cells, Animals, Chemotaxis, Leukocyte, Disease Models, Animal, Humans, Neoplasms, Neutrophils, Sulfonamides, Biomarkers, T-Lymphocyte Subsets, Interleukin-8, Mice, Knockout, Cell Line, Tumor, Mice, Extracellular Traps

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