The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Nuclease Expression by Staphylococcus aureus Facilitates Escape from Neutrophil Extracellular Traps
Open accessAbstract
Neutrophils are key effectors of the host innate immune response against bacterial infection. Staphylococcus aureus is a preeminent human pathogen, with an ability to produce systemic infections even in previously healthy individuals, thereby reflecting a resistance to effective neutrophil clearance. The recent discovery of neutrophil extracellular traps (NETs) has opened a novel dimension in our understanding of how these specialized leukocytes kill pathogens. NETs consist of a nuclear DNA backbone associated with antimicrobial peptides, histones and proteases that provide a matrix to entrap and kill various microbes. Here, we used targeted mutagenesis to examine a potential role of S. aureus nuclease in NET degradation and virulence in a murine respiratory tract infection model. In vitro assays using fluorescence microscopy showed the isogenic nuclease-deficient (nuc-deficient) mutant to be significantly impaired in its ability to degrade NETs compared with the wild-type parent strain USA 300 LAC. Consequently, the nuc-deficient mutant strain was significantly more susceptible to extracellular killing by activated neutrophils. Moreover, S. aureus nuclease production was associated with delayed bacterial clearance in the lung and increased mortality after intranasal infection. In conclusion, this study shows that S. aureus nuclease promotes resistance against NET-mediated antimicrobial activity of neutrophils and contributes to disease pathogenesis in vivo.
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Cites
Neutrophil Extracellular Traps Kill Bacteria
Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D. S., Weinrauch, Y., & Zychlinsky, A. (2004) · Science · doi:10.1126/science.1092385 · Cited by 10,268
Waves of resistance: Staphylococcus aureus in the antibiotic era
Chambers, H. F., & DeLeo, F. R. (2009) · Nature Reviews Microbiology · doi:10.1038/nrmicro2200 · Cited by 2,718
Miller, L. G., Perdreau-Remington, F., Rieg, G., Mehdi, S., Perlroth, J., Bayer, A. S., Tang, A. W., Phung, T. O., & Spellberg, B. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa042683 · Cited by 1,053
Dorschner, R. A., Pestonjamasp, V. K., Tamakuwala, S., Ohtake, T., Rudisill, J., Nizet, V., Agerberth, B., Gudmundsson, G. H., & Gallo, R. L. (2001) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.2001.01340.x · Cited by 567
Self-citationInsights into Mechanisms Used by Staphylococcus aureus to Avoid Destruction by Human Neutrophils
Voyich, J. M., Braughton, K. R., Sturdevant, D. E., Whitney, A. R., Saïd-Salim, B., Porcella, S. F., Long, R. D., Dorward, D. W., Gardner, D. J., Kreiswirth, B. N., et al. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.6.3907 · Cited by 563
The IL-8 Protease SpyCEP/ScpC of Group A Streptococcus Promotes Resistance to Neutrophil Killing
Zinkernagel, A. S., Timmer, A. M., Pence, M. A., Locke, J. B., Buchanan, J. T., Turner, C. E., Mishalian, I., Sriskandan, S., Hanski, E., & Nizet, V. (2008) · Cell Host & Microbe · doi:10.1016/j.chom.2008.07.002 · Cited by 184
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Cited by
Pathogenicity and virulence of Staphylococcus aureus
Cheung, G. Y. C., Bae, J. S., & Otto, M. (2021) · Virulence · doi:10.1080/21505594.2021.1878688 · Cited by 1,380
Diabetes primes neutrophils to undergo NETosis, which impairs wound healing
Wong, S. L., Demers, M., Martinod, K., Gallant, M., Wang, Y., Goldfine, A. B., Kahn, C. R., & Wagner, D. D. (2015) · Nature Medicine · doi:10.1038/nm.3887 · Cited by 1,264
Infection-induced NETosis is a dynamic process involving neutrophil multitasking in vivo
Yipp, B. G., Petri, B., Salina, D., Jenne, C. N., Scott, B. N. V., Zbytnuik, L. D., Pittman, K., Asaduzzaman, M., Wu, K., Meijndert, H. C., et al. (2012) · Nature Medicine · doi:10.1038/nm.2847 · Cited by 1,169
Neutrophil extracellular traps: Is immunity the second function of chromatin?
Brinkmann, V., & Zychlinsky, A. (2012) · Journal of Cell Biology · doi:10.1083/jcb.201203170 · Cited by 1,021
Yipp, B. G., & Kubes, P. (2013) · Blood · doi:10.1182/blood-2013-04-457671 · Cited by 989
Metadata
- Authors
- Berends, E. T. M., Horswill, A. R., Haste, N. M., Monestier, M., Nizet, V., & von Köckritz-Blickwede, M.
- Venue
- Journal of Innate Immunity
- Publisher
- S. Karger AG
- Published
- 2010
- Volume
- 2
- Issue
- 6
- Pages
- 576-586
- ISSN
- 1662-811X (ISSN-L)
- 1662-8128
- OpenAlex
- W2063966020
- Cited by
- 487
- References
- 6
- FWCI
- 8.20
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Berends, E. T. M., Horswill, A. R., Haste, N. M., Monestier, M., Nizet, V., & von Köckritz-Blickwede, M. (2010). Nuclease Expression by Staphylococcus aureus Facilitates Escape from Neutrophil Extracellular Traps. Journal of Innate Immunity. https://doi.org/10.1159/000319909
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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