Cited by 78Endometriosis Research and TreatmentCancer-related molecular mechanisms researchReproductive System and Pregnancy

Genome-Wide Microarray Analysis of Long Non-Coding RNAs in Eutopic Secretory Endometrium with Endometriosis

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Wang, Yang, Li, Yan, Yang, Zhuo, Liu, Kuiran, Wang, Danbo·2015·Cellular Physiology and Biochemistry

Abstract

BACKGROUND/AIMS: Dysregulated long non-coding RNAs (lncRNAs) can lead to the occurrence of various diseases; however, reports of the function of lncRNAs in endometriosis and related studies are scarce. The pathogenesis of endometriosis is still poorly understood. METHODS: Dysregulated lncRNAs and mRNAs between eutopic and normal endometrium (both are late secretory) were analyzed by lncRNA microarray. Eight lncRNAs and mRNA CDK6 were validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatics prediction was used to investigate the potential function of these differentially expressed lncRNAs. RESULTS: Microarray expression profiling suggests 1277 lncRNAs (488 up- and 789 down-regulated) and 1216 mRNAs (578 up- and 638 down-regulated) were expressed differentially between eutopic and normal endometrium. Pathway analysis and gene ontology (GO) analysis found differently expressed lncRNAs associated with the cell cycle and immune regulation. The relative level of expression of CDK6 and AC002454.1 were obtained by qRT-PCR and the Pearson correlation coefficient was 0.747 (p<0.0001). A coding-noncoding gene co-expression (CNC) network was constructed for these validated lncRNAs. CONCLUSION: These dysregulated lncRNAs might provide information for new biomarkers or novel therapeutic targets of endometriosis. AC002454.1 might induce cell cycle disorder by regulating CDK6 to participate in the pathogenesis of endometriosis.

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  • Wang et al. in 2015 found 1,277 lncRNAs that were dysregulated in the secretory-phase eutopic endometrium of patients compared with the menstrual-phase-matched endometrium from a control cohort.

    “Using genome-wide microarray approach, Wang et al. in 2015 (113) found 1,277 lncRNAs that were dysregulated in the secretory-phase eutopic endometrium of patients compared with the menstrual-phase-matched endometrium from a control cohort.”

    From Biomarkers in endometriosis: Challenges and opportunities (2017) · cites this paper as [113]

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Research works printed but not linked: 2

  1. Giudice LC, Kao LC: Endometriosis. The Lancet 2004;364:1789-1799.
  2. Meng LH, Zhang H, Hayward L, Takemura H, Shao RG, Pommier Y: Tetrandrine induces early G1 arrest in cyclin-dependent kinases and by inducing p53 and p21Cip1. Cancer Res 2004;64:9086-9092.

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Authors
Wang, Y., Li, Y., Yang, Z., Liu, K., & Wang, D.
Affiliations
Sheng Jing Hospital (CN)
Venue
Cellular Physiology and Biochemistry
Publisher
S. Karger AG
Published
2015
Volume
37
Issue
6
Pages
2231-2245
ISSN
  • 1015-8987 (ISSN-L)
  • 1421-9778
OpenAlex
W2267639294
Cited by
78
References
50
Citations by year
2026: 1 · 2025: 4 · 2024: 4 · 2023: 9 · 2022: 2 · 2021: 10 · 2020: 15 · 2019: 12 · 2018: 8 · 2017: 7 · 2016: 5 · 2015: 1
FWCI
7.98
Type
journal article
Language
en
Open access
Locations
  • Cellular Physiology and Biochemistry (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (201204, 81270675)
  • Shengjing Hospital
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
Keywords
endometriosis, long non-coding RNA, secretory endometrium, eutopic endometrium, differentially expressed lncRNAs, CDK6, cell cycle regulation, immune regulation, biomarkers, therapeutic target
MeSH terms
Adult, Endometriosis, Endometrium, Female, Humans, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Genome-Wide Association Study, RNA, Long Noncoding

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