Genome-Wide Microarray Analysis of Long Non-Coding RNAs in Eutopic Secretory Endometrium with Endometriosis
Open accessAbstract
BACKGROUND/AIMS: Dysregulated long non-coding RNAs (lncRNAs) can lead to the occurrence of various diseases; however, reports of the function of lncRNAs in endometriosis and related studies are scarce. The pathogenesis of endometriosis is still poorly understood. METHODS: Dysregulated lncRNAs and mRNAs between eutopic and normal endometrium (both are late secretory) were analyzed by lncRNA microarray. Eight lncRNAs and mRNA CDK6 were validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatics prediction was used to investigate the potential function of these differentially expressed lncRNAs. RESULTS: Microarray expression profiling suggests 1277 lncRNAs (488 up- and 789 down-regulated) and 1216 mRNAs (578 up- and 638 down-regulated) were expressed differentially between eutopic and normal endometrium. Pathway analysis and gene ontology (GO) analysis found differently expressed lncRNAs associated with the cell cycle and immune regulation. The relative level of expression of CDK6 and AC002454.1 were obtained by qRT-PCR and the Pearson correlation coefficient was 0.747 (p<0.0001). A coding-noncoding gene co-expression (CNC) network was constructed for these validated lncRNAs. CONCLUSION: These dysregulated lncRNAs might provide information for new biomarkers or novel therapeutic targets of endometriosis. AC002454.1 might induce cell cycle disorder by regulating CDK6 to participate in the pathogenesis of endometriosis.
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Wang et al. in 2015 found 1,277 lncRNAs that were dysregulated in the secretory-phase eutopic endometrium of patients compared with the menstrual-phase-matched endometrium from a control cohort.
“Using genome-wide microarray approach, Wang et al. in 2015 (113) found 1,277 lncRNAs that were dysregulated in the secretory-phase eutopic endometrium of patients compared with the menstrual-phase-matched endometrium from a control cohort.”
From Biomarkers in endometriosis: Challenges and opportunities (2017) · cites this paper as [113]
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- Authors
- Wang, Y., Li, Y., Yang, Z., Liu, K., & Wang, D.
- Affiliations
- Sheng Jing Hospital (CN)
- Venue
- Cellular Physiology and Biochemistry
- Publisher
- S. Karger AG
- Published
- 2015
- Volume
- 37
- Issue
- 6
- Pages
- 2231-2245
- ISSN
- 1015-8987 (ISSN-L)
- 1421-9778
- OpenAlex
- W2267639294
- Cited by
- 78
- References
- 50
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 9 · 2022: 2 · 2021: 10 · 2020: 15 · 2019: 12 · 2018: 8 · 2017: 7 · 2016: 5 · 2015: 1
- FWCI
- 7.98
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cellular Physiology and Biochemistry (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (201204, 81270675)
- Shengjing Hospital
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Keywords
- endometriosis, long non-coding RNA, secretory endometrium, eutopic endometrium, differentially expressed lncRNAs, CDK6, cell cycle regulation, immune regulation, biomarkers, therapeutic target
- MeSH terms
- Adult, Endometriosis, Endometrium, Female, Humans, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Genome-Wide Association Study, RNA, Long Noncoding
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