Cited by 49Endometriosis Research and TreatmentMicroRNA in disease regulationExtracellular vesicles in disease

Expression of miR-126 and Crk in endometriosis: miR-126 may affect the progression of endometriosis by regulating Crk expression

Liu, Shuang, Gao, Shan, Wang, Xiao Ying, Wang, Dan Bo·2012·Archives of Gynecology and Obstetrics

Abstract

Purpose To evaluate the relationship between miR-126 and Crk and discuss the role of miR-126 in the development and progression of endometriosis (EMs).Methods The expression levels of miR-126 and Crk mRNA were quantified using real time fluorescent quantitative polymerase chain reaction (real time PCR) in ectopic endometrium (ECs) and eutopic endometrium (EUs) in patients with EMs and normal endometrium (ENs) in EMs-free subjects. The expression levels of Crk protein in all samples were evaluated by Western blot.Results The expression level of miR-126 was significantly downregulated in ECs versus EUs (p = 5.45E(-5)) in the experimental group and in EUs versus ENs (p = 0.019). The expression level of Crk mRNA did not distinguish ECs from EUs (p = 0.995) but was overexpressed in EUs versus ENs (p = 0.006). Crk protein was overexpressed in ECs versus EUs (p = 0.002) in the experimental group and in EUs versus ENs (p = 1.13E(-6)). The expression level of miR-126 had no correlation with Crk mRNA (p = 0.496) but was negatively correlated with Crk protein (p = 3.134E(-5)). The expression level of miR-126 in EUs and ECs was negatively correlated with American Fertility Society (AFS) stage (p = 0.022, p = 0.025) and AFS score (p = 0.002, p = 0.007). miR-126 expression decreased with the progression of EMs, but the decrease was not significantly different.Conclusions miR-126 may play an initial role in the development and progression of EMs. Crk may be regulated by miR-126, and synergism between abnormal expressions may play an important role in the pathogenesis of EMs.

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Crossref · 10.1007/s00404-011-2112-6

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  • Other miRNAs and predicted cognate mRNAs demonstrating differential expression in eutopic endometrium from women with and without endometriosis include miR-23a/CYP19A1 and miR-542-3p/COX2, miR-126/CRK and miR23a/NR5A1.

    “Other miRNAs and predicted cognate mRNAs demonstrating differential expression in eutopic endometrium from women with and without endometriosis include miR-23a/CYP19A1 and miR-542-3p/COX2 [128], miR-126/CRK [129] and miR23a/NR5A1 [130].”

    From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [129]

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Authors
Liu, S., Gao, S., Wang, X. Y., & Wang, D. B.
Affiliations
Sheng Jing Hospital (CN)
Corresponding author
Wang, Dan Bo
Venue
Archives of Gynecology and Obstetrics
Published
2011-10-20
Volume
285
Issue
4
Pages
1065-1072
ISSN
  • 0003-9128 (ISSN-L)
  • 0932-0067
  • 1432-0711
OpenAlex
W2075614252
Cited by
49
References
36
Citations by year
2025: 2 · 2024: 3 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 5 · 2018: 4 · 2017: 3 · 2016: 1 · 2015: 7 · 2014: 3 · 2013: 4 · 2012: 1
FWCI
2.86
Type
journal article
Language
en
Open access
closed
Locations
  • Archives of Gynecology and Obstetrics (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
miR-126, Crk, endometriosis, ectopic endometrium, eutopic endometrium, microRNA regulation, endometriosis progression, endometriosis pathogenesis
MeSH terms
Adult, Endometriosis, Female, Humans, Disease Progression, MicroRNAs, Proto-Oncogene Proteins c-crk

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