Increase of circulating endothelial progenitor cells in patients with coronary artery disease after exercise-induced ischemia
Abstract
OBJECTIVE: The concept of neovascularization in response to tissue ischemia has been extended by the finding of postnatal vasculogenesis initiated by endothelial progenitor cells (EPCs). The aim of this study was to analyze whether a maximal stress test in patients with coronary artery disease (CAD) increases the number of circulating EPCs. METHODS AND RESULTS: Blood concentration of EPCs was analyzed by FACS and cell culture assay in CAD patients with (n=16) or without (n=12) exercise-induced myocardial ischemia and in healthy subjects (n=11) for up to 144 hours after maximal stress test. Plasma concentrations of vascular endothelial growth factor (VEGF), basic fibroblast growth factor, tumor necrosis factor-alpha, and granulocyte macrophage-colony stimulating factor were determined by ELISA. EPCs increased significantly in ischemic patients, with a maximum after 24 to 48 hours (cell culture: 3.3+/-0.5-fold increase; FACS: 3.1+/-0.6-fold increase) and returned to baseline within 72 hour. In nonischemic patients and healthy subjects, no EPC increase was detectable. VEGF levels in ischemic patients increased significantly after 2 to 6 hours (maximum after 2 hours; 4.0+/-1.1-fold increase) and no change was observed in nonischemic patients and healthy subjects; DeltaVEGF and DeltaEPC correlated significantly (r=0.66). CONCLUSIONS: Patients with symptomatic CAD respond to a single episode of exercise-induced myocardial ischemia with a time-dependent increase in circulating EPCs. This increase may be related to and preceded by an increase in plasma VEGF.
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Exercise-particularly if intense enough to induce transient myocardial ischaemia-can stimulate circulating angiogenic progenitor cells, thereby increasing the number and/or function of these cells in people with cardiometabolic conditions such as metabolic syndrome or obesity.
“Exercise-particularly if intense enough to induce transient myocardial ischaemia 169,170 -can also stimulate circulating angiogenic progenitor cells (whose levels are inversely associated with CVD risk 171 ), thereby increasing the number and/or function of these cells in people with cardiometabolic conditions such as metabolic syndrome or obesity.169”
From Exercise benefits in cardiovascular diseases: From mechanisms to clinical implementation (2023) · cites this paper as [170]
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- Authors
- Adams, V., Lenk, K., Linke, A., Lenz, D., Erbs, S., Sandri, M., Tarnok, A., Gielen, S., Emmrich, F., Schuler, G., & Hambrecht, R.
- Affiliations
- Leipzig University (DE)
- From University Leipzig (V.A., K.L., S.E., M.S., S.G., G.S., R.H.), Heart Center Leipzig, Clinic of Cardiology, and Pediatric Cardiology (D.L., A.T.), Germany; and Institute of Clinical Immunology and Transfusion Medicine (F.E.) University Leipzig, Germany.
- Venue
- Arteriosclerosis Thrombosis and Vascular Biology
- Published
- 2004-04
- Volume
- 24
- Issue
- 4
- Pages
- 684-690
- ISSN
- 1079-5642 (ISSN-L)
- 1524-4636
- OpenAlex
- W2159538020
- Cited by
- 367
- References
- 39
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 2 · 2023: 5 · 2022: 7 · 2021: 3 · 2020: 4 · 2019: 11 · 2018: 9 · 2017: 11 · 2016: 13 · 2015: 20 · 2014: 18 · 2013: 18 · 2012: 34
- FWCI
- 10.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- endothelial progenitor cells, coronary artery disease, exercise-induced myocardial ischemia, stress testing, vascular endothelial growth factor, neovascularization, vasculogenesis, growth factors
- MeSH terms
- Aged, Angina Pectoris, Cell Differentiation, Cells, Cultured, Endothelium, Vascular, Exercise Test, Physical Exertion, Female, Flow Cytometry, Humans, Male, Middle Aged, Myocardial Revascularization, Tumor Necrosis Factor-alpha, Granulocyte-Macrophage Colony-Stimulating Factor, Fibroblast Growth Factor 2, Myocardial Ischemia, Pluripotent Stem Cells, Vascular Endothelial Growth Factor A, Mesenchymal Stem Cells
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