Haematopoietic stem cells and endothelial progenitor cells in healthy men: effect of aging and training
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Summary: The number of hematopoietic stem cells (HSC) and endothelial progenitor cells (EPC) is thought to be a marker for neovascularization and vascular repair. Because physical inactivity and aging are risk factors for cardiovascular diseases, these factors may influence the numbers of HSCs and EPCs. Therefore, we examined baseline and exercise‐induced levels of HSCs and EPCs in sedentary and trained young and older men. To study the role of aging in eight sedentary young (19–28 years) and eight sedentary older men (67–76 years), baseline and acute exercise‐induced numbers of HSCs (CD34+‐cells) and EPCs (CD34+/VEGFR‐2+‐cells) were quantified by fluorescence‐activated cell sorter (FACS) analysis. To examine the effect of chronic training, eight age‐matched trained young men (18–28 years) were compared with sedentary young men, whereas older men performed an 8‐week endurance training. Older men showed significantly lower baseline and exercise‐induced levels of HSCs/EPCs than the young men (P < 0.05). In young and older men, acute exercise significantly increased HSCs (P < 0.01), but not EPCs. The absolute increase in numbers of HSCs was attenuated in older men (P = 0.03). Apart from the lower baseline numbers of EPCs after chronic training in older men, training status did not alter baseline or exercise‐induced levels of HSCs/EPCs in young and older men. We concluded that advancing age results in lower circulating numbers of HSCs and EPCs and attenuates the acute exercise‐induced increase in HSCs. Interestingly, in young as well as in older men chronic endurance training does not affect baseline and exercise‐induced numbers of HSCs and EPCs.
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The acute effects of exercise include improvements in the trafficking and functional capabilities of many cell types, including haematopoietic stem cells, cells of myeloid lineage, and cells of lymphoid lineage.
“The acute effects of exercise include improvements in the trafficking and functional capabilities of many cell types, including haematopoietic stem cells (Thijssen et al. 2006), cells of myeloid lineage (e.g., monocytes, neutrophils, dendritic cells) and cells of lymphoid lineage (e.g., NK cells, B cells and T cells) (Shephard 2003; Walsh et al. 2011b).”
From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Thijssen et al. 2006
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Cites
Isolation of Putative Progenitor Endothelial Cells for Angiogenesis
Asahara, T., Murohara, T., Sullivan, A., Silver, M., van der Zee, R., Li, T., Witzenbichler, B., Schatteman, G., & Isner, J. M. (1997) · Science · doi:10.1126/science.275.5302.964 · Cited by 8,725 · see citing works
Bone marrow cells regenerate infarcted myocardium
Orlic, D., Kajstura, J., Chimenti, S., Jakoniuk, I., Anderson, S. M., Li, B., Pickel, J. M., McKay, R. D. G., Nadal‐Ginard, B., Bodine, D. M., et al. (2001) · Nature · doi:10.1038/35070587 · Cited by 5,149 · see citing works
Circulating endothelial progenitor cells, vascular function, and cardiovascular risk
Hill, J. M., Zalos, G., Halcox, J. P. J., Schenke, W. H., Waclawiw, M. A., Quyyumi, A. A., & Finkel, T. (2003) · New England Journal of Medicine · doi:10.1056/nejmoa022287 · Cited by 3,423 · see citing works
Physical Activity, All-Cause Mortality, and Longevity of College Alumni
Paffenbarger, R. S., Hyde, R., Wing, A. L., & Hsieh, C.-C. (1986) · New England Journal of Medicine · doi:10.1056/nejm198603063141003 · Cited by 2,742 · see citing works
Urbich, C., & Dimmeler, S. (2004) · Circulation Research · doi:10.1161/01.res.0000137877.89448.78 · Cited by 2,364 · see citing works
Vasa‐Nicotera, M., Fichtlscherer, S., Aicher, A., Adler, K., Urbich, C., Martin, H., Zeiher, A. M., & Dimmeler, S. (2001) · Circulation Research · doi:10.1161/hh1301.093953 · Cited by 2,328 · see citing works
Circulating endothelial progenitor cells and cardiovascular outcomes
Werner, N., Kosiol, S., Schiegl, T., Ahlers, P., Walenta, K., Link, A., Böhm, M., & Nickenig, G. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa043814 · Cited by 2,048 · see citing works
Asahara, T., Takahashi, T., Masuda, H., Kalka, C., Chen, D., Iwaguro, H., Inai, Y., Silver, M., & Isner, J. M. (1999) · The EMBO Journal · doi:10.1093/emboj/18.14.3964 · Cited by 1,856 · see citing works
Celermajer, D. S., Sørensen, K. E., Spiegelhalter, D. J., Georgakopoulos, D., Robinson, J. T. C., & Deanfield, J. E. (1994) · Journal of the American College of Cardiology · doi:10.1016/0735-1097(94)90305-0 · Cited by 1,334 · see citing works
Mobilization of Endothelial Progenitor Cells in Patients With Acute Myocardial Infarction
Shintani, S., Murohara, T., Ikeda, H., Ueno, T., Honma, T., Katoh, A., Sasaki, K., Shimada, T., Oike, Y., & Imaizumi, T. (2001) · Circulation · doi:10.1161/hc2301.092122 · Cited by 1,185 · see citing works
Aging, Habitual Exercise, and Dynamic Arterial Compliance
Tanaka, H., Dinenno, F. A., Monahan, K. D., Clevenger, C. M., DeSouza, C. A., & Seals, D. R. (2000) · Circulation · doi:10.1161/01.cir.102.11.1270 · Cited by 1,116 · see citing works
Reduced Number of Circulating Endothelial Progenitor Cells Predicts Future Cardiovascular Events
Schmidt‐Lucke, C., Rössig, L., Fichtlscherer, S., Vasa‐Nicotera, M., Britten, M. B., Kämper, U., Dimmeler, S., & Zeiher, A. M. (2005) · Circulation · doi:10.1161/circulationaha.104.504340 · Cited by 1,060 · see citing works
DeSouza, C. A., Shapiro, L. F., Clevenger, C. M., Dinenno, F. A., Monahan, K. D., Tanaka, H., & Seals, D. R. (2000) · Circulation · doi:10.1161/01.cir.102.12.1351 · Cited by 896 · cited 1× · see citing works
“Moreover, the ∼8% increase in oxygen consumption is larger than reported in previous studies in which older men were trained for 12 weeks (DeSouza et al., 2000; Tanaka et al., 2000; Tanaka et al., 2002).”
ResultLaufs, U., Werner, N., Link, A., Endres, M., Waßmann, S., Jürgens, K., Miche, E., Böhm, M., & Nickenig, G. (2003) · Circulation · doi:10.1161/01.cir.0000109141.48980.37 · Cited by 858 · see citing works
Aging, Progenitor Cell Exhaustion, and Atherosclerosis
Rauscher, F. M., Goldschmidt‐Clermont, P. J., Davis, B. H., Wang, T., Gregg, D., Ramaswami, P., Pippen, A. M., Annex, B. H., Dong, C., & Taylor, D. A. (2003) · Circulation · doi:10.1161/01.cir.0000082924.75945.48 · Cited by 674 · see citing works
Impaired progenitor cell activity in age-related endothelial dysfunction
Heiss, C., Keymel, S., Niesler, U., Ziemann, J., Kelm, M., & Kalka, C. (2005) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2004.12.074 · Cited by 573 · see citing works
Unfavorable Effects of Resistance Training on Central Arterial Compliance
Miyachi, M., Kawano, H., Sugawara, J., Takahashi, K., Hayashi, K., Yamazaki, K., Tabata, I., & Tanaka, H. (2004) · Circulation · doi:10.1161/01.cir.0000146380.08401.99 · Cited by 498 · see citing works
Physical Activity Prevents Age-Related Impairment in Nitric Oxide Availability in Elderly Athletes
Taddei, S., Galetta, F., Virdis, A., Ghiadoni, L., Salvetti, G., Franzoni, F., Giusti, C., & Salvetti, A. (2000) · Circulation · doi:10.1161/01.cir.101.25.2896 · Cited by 460 · see citing works
Adams, V., Lenk, K., Linke, A., Lenz, D., Erbs, S., Sandri, M., Tarnok, A., Gielen, S., Emmrich, F., Schuler, G., et al. (2004) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000124104.23702.a0 · Cited by 367 · see citing works
Eskurza, I., Monahan, K. D., Robinson, J. A., & Seals, D. R. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2003.057042 · Cited by 341 · see citing works
Rehman, J., Li, J., Parvathaneni, L., Karlsson, G., Panchal, V. R., Temm, C. J., Mahenthiran, J., & March, K. L. (2004) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2004.02.049 · Cited by 322 · see citing works
Bone marrow stem cells regenerate infarcted myocardium
Orlic, D., Kajstura, J., Chimenti, S., et al. (2003) · Pediatric Transplantation · doi:10.1034/j.1399-3046.7.s3.13.x · Cited by 288 · see citing works
Sandri, M., Adams, V., Gielen, S., Linke, A., Lenk, K., Kränkel, N., Lenz, D., Erbs, S., Scheinert, D., Mohr, F. W., et al. (2005) · Circulation · doi:10.1161/circulationaha.104.527135 · Cited by 280 · see citing works
Steiner, S., Niessner, A., Ziegler, S., Richter, B., Seidinger, D., Pleiner, J., Penka, M., Wolzt, M., Huber, K., Wojta, J., et al. (2005) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2005.01.006 · Cited by 250 · see citing works
Monahan, K. D., Tanaka, H., Dinenno, F. A., et al. (2001) · Circulation · doi:10.1161/hc3901.096670 · Cited by 228 · see citing works
Laufs, U., Urhausen, A. J. P., Werner, N., Scharhag, J., Heitz, A., Kissner, G., Böhm, M., Kindermann, W., & Nickenig, G. (2005) · European Journal of Cardiovascular Prevention & Rehabilitation · doi:10.1097/01.hjr.0000174823.87269.2e · Cited by 212 · see citing works
Circulating haemopoietic and endothelial progenitor cells are decreased in COPD
Palange, P., Testa, U., Huertas, A., Calabrò, L., Antonucci, R., Petrucci, E., Pelosi, E., Pasquini, L., Satta, A., Morici, G., et al. (2006) · European Respiratory Journal · doi:10.1183/09031936.06.00120604 · Cited by 192 · see citing works
Circulating plasma VEGF response to exercise in sedentary and endurance-trained men
Kraus, R. M., Stallings, H. W., Yeager, R. C., & Gavin, T. P. (2004) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01031.2003 · Cited by 187 · see citing works
Lower skeletal muscle capillarization and VEGF expression in aged vs. young men
Ryan, N. A., Zwetsloot, K. A., Westerkamp, L. M., Hickner, R. C., Pofahl, W. E., & Gavin, T. P. (2005) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00827.2005 · Cited by 165 · see citing works
Tanaka, H., Seals, D. R., Monahan, K. D., Clevenger, C. M., DeSouza, C. A., & Dinenno, F. A. (2002) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00824.2001 · Cited by 153 · see citing works
Angiogenic growth factor response to acute systemic exercise in human skeletal muscle
Gavin, T. P., Robinson, C. B., Yeager, R. C., England, J. A., Nifong, L. W., & Hickner, R. C. (2004) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00748.2003 · Cited by 136 · see citing works
Ishizawa, K., Kubo, H., Yamada, M., Kobayashi, S., SUZUKI, T. A., Mizuno, S., Nakamura, T., & Sasaki, H. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.09.049 · Cited by 133 · see citing works
Supramaximal exercise mobilizes hematopoietic progenitors and reticulocytes in athletes
Morici, G., Zangla, D., Santoro, A., Pelosi, E., Petrucci, E., Gioia, M., Bonanno, A., Profita, M., Bellia, V., Testa, U., et al. (2005) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00338.2005 · Cited by 112 · see citing works
Özüyaman, B., Ebner, P., Niesler, U., Ziemann, J., Kleinbongard, P., Jax, T. W., Gödecke, A., Kelm, M., & Kalka, C. (2005) · Thrombosis and Haemostasis · doi:10.1160/th05-01-0038 · Cited by 94 · see citing works
GREEN, D. J., Walsh, J. H., Maiorana, A., Burke, V., Taylor, R. R., & O'Driscoll, J. G. (2004) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00109.2004 · Cited by 86 · see citing works
Shaffer, R. G., Greene, S., Arshi, A., Supple, G., Bantly, A. D., Moore, J. S., & Mohler, E. R. (2006) · Cytometry Part B Clinical Cytometry · doi:10.1002/cyto.b.20085 · Cited by 69 · see citing works
Domain processes in the magnetization reversal of exchange-biased IrMn/CoFe bilayers
Gogol, P., Chapman, J. N., Gillies, M. F., et al. (2002) · Journal of Applied Physics · doi:10.1063/1.1489494 · Cited by 49 · see citing works
Franzoni, F., Plantinga, Y., Femia, F. R., Bartolomucci, F., Gaudio, C., Regoli, F., Carpi, A., Santoro, G., & Galetta, F. (2004) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2004.08.009 · Cited by 46 · see citing works
Span, P. N., Grebenchtchikov, N., Geurts-Moespot, J., et al. (2000) · The International Journal of Biological Markers · doi:10.1177/172460080001500210 · Cited by 45 · see citing works
No Age-Related Change in Circulating Endothelial Progenitor Cells in Healthy Subjects
Chen, M., Yip, H., Chen, C., et al. (2006) · International Heart Journal · doi:10.1536/ihj.47.95 · Cited by 15 · see citing works
Franzoni, F., PLANTINGA, Y., Femia, F. R., et al. (2004) · Biomedicine & Pharmacotherapy · doi:10.1016/s0753-3322(04)00115-5 · Cited by 14 · see citing works
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- Authors
- Thijssen, D. H. J., Vos, J. B., Verseyden, C., Van Zonneveld, A. J., Smits, P., Sweep, F. C. G. J., Hopman, M. T. E., & De Boer, H. C.
- Affiliations
- Radboud University Medical Center (NL)
- Leiden University Medical Center (NL)
- Corresponding author
- Thijssen, Dick H. J.
- Venue
- Aging Cell
- Publisher
- Wiley
- Published
- 2006-10-20
- Volume
- 5
- Issue
- 6
- Pages
- 495-503
- ISSN
- 1474-9718 (ISSN-L)
- 1474-9726
- OpenAlex
- W2071435082
- Cited by
- 156
- References
- 41
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 3 · 2022: 8 · 2021: 3 · 2020: 5 · 2019: 13 · 2018: 11 · 2017: 5 · 2016: 7 · 2015: 10 · 2014: 4 · 2013: 9 · 2012: 15
- FWCI
- 3.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Aging Cell (publishedVersion)
- PubMed (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion, other-oa)
- Pure Amsterdam UMC (submittedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- hematopoietic stem cells, endothelial progenitor cells, aging, endurance training, acute exercise, sedentary lifestyle, CD34+ cells, VEGFR2, vascular repair, neovascularization, cardiovascular risk factors, older men
- MeSH terms
- Adult, Aged, Aging, Cell Count, Exercise Therapy, Flow Cytometry, Hematopoietic Stem Cells, Humans, Male, Physical Fitness, Stem Cells, Up-Regulation, Exercise Tolerance, Neovascularization, Physiologic, Endothelial Cells, Cell Proliferation
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