Cited by 103Cholesterol and Lipid MetabolismAtherosclerosis and Cardiovascular DiseasesLipoproteins and Cardiovascular Health

Arterial Permeability and Efflux of Apolipoprotein B–Containing Lipoproteins Assessed by In Situ Perfusion and Three-Dimensional Quantitative Confocal Microscopy

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Proctor, Spencer D., Vine, Donna F., Mamo, John C.L.·2004·Arteriosclerosis, Thrombosis, and Vascular Biology

Abstract

Objectives— There is accumulating evidence that an increased risk of cardiovascular disease (CVD) is not simply caused by the degree of arterial exposure to plasma lipoproteins but, in addition, is determined by the affinity of the vasculature for different lipoprotein phenotypes. In this study we compare the delivery and efflux of 2 atherogenic lipoproteins to further understand the factors that regulate cholesterol accumulation in early atherogenesis. Methods and Results— Lipoproteins containing apolipoprotein (apo) B100 (a low-density lipoprotein [LDL]) and apoB48 (chylomicron remnants) were isolated and differentially conjugated with fluorophores and simultaneously perfused at equivalent concentrations in situ through rabbit carotid vessels. Perfusion systems were established to quantify and differentiate between lipoprotein arterial delivery and efflux. The total average rate of delivery for LDL particles (23 nm) compared with chylomicron remnants (50 nm) was 4427 particles/min −1 per μm 3 and 452 particles/min −1 per μm 3 , respectively. In contrast, the average rate of efflux was 3195 particles/min −1 per μm 3 and 163 particles/min −1 per μm 3 for LDL and chylomicron remnants, respectively. Conclusions— Results indicate that although LDL particles have a higher rate of delivery, they efflux more readily from arterial tissue compared with the larger chylomicron remnants. Collectively, our findings highlight that lipoproteins permeate through arterial tissue differently and may be dependent on the phenotype and potential interactions with extracellular matrix components.

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  1. Mamo JCL, Proctor SD. Coronary artery disease; which lipoprotein is the villain. Life Science. 2002;14:30 -33.
  2. Olin KL, Potter-Perigo S, Barret PH, Wight TN, Chait A. Biglycan, a vascular proteoglycan, binds differently to HDL2 and HDL3: role of apoE. Arterioscler Thromb Vasc Biol. 2001;21:129 -135.

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Authors
Proctor, S. D., Vine, D. F., & Mamo, J. C. L.
Affiliations
Curtin University (AU)
Venue
Arteriosclerosis, Thrombosis, and Vascular Biology
Published
2004-11
Volume
24
Issue
11
Pages
2162-2167
ISSN
  • 1079-5642 (ISSN-L)
  • 1524-4636
OpenAlex
W1966788643
Cited by
103
References
30
Citations by year
2025: 5 · 2024: 4 · 2023: 7 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 6 · 2017: 3 · 2015: 4 · 2014: 1 · 2013: 4 · 2012: 4
FWCI
3.47
Type
journal article
Language
en
Open access
Locations
  • Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Heart Foundation of Australia
  • Medical Research Council
  • National Health and Medical Research Council (229030)
  • National Medical Research Council
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
Keywords
arterial permeability, apolipoprotein B-100, apolipoprotein B-48, chylomicron remnants, low-density lipoprotein, atherogenesis, cholesterol accumulation, carotid artery, in situ perfusion, extracellular matrix, lipoprotein phenotype
MeSH terms
Animals, Apolipoproteins B, Capillary Permeability, Humans, Infusions, Intra-Arterial, Lipoproteins, LDL, Particle Size, Rabbits, Microscopy, Confocal, Imaging, Three-Dimensional

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