ATP-Binding Cassette Transporter G1 and High-Density Lipoprotein Promote Endothelial NO Synthesis Through a Decrease in the Interaction of Caveolin-1 and Endothelial NO Synthase
Open accessAbstract
Objective— To investigate whether cholesterol efflux to high-density lipoprotein (HDL) via ATP-binding cassette transporter G1 (ABCG1) modulates the interaction of caveolin (Cav) 1 and endothelial NO synthase (eNOS). Methods and Results— ABCG1 promotes cholesterol and 7-oxysterol efflux from endothelial cells (ECs) to HDL. It was previously reported that ABCG1 protects against dietary cholesterol-induced endothelial dysfunction by promoting the efflux of 7-oxysterols to HDL. Increased cholesterol loading in ECs is known to cause an inhibitory interaction between Cav-1 and eNOS and impaired NO release. In human aortic ECs, free cholesterol loading promoted the interaction of Cav-1 with eNOS, reducing eNOS activity. These effects of cholesterol loading were reversed by HDL in an ABCG1-dependent manner. HDL also reversed the inhibition of eNOS by cholesterol loading in murine lung ECs, but this effect of HDL was abolished in Cav-1–deficient murine lung ECs. Increased interaction of Cav-1 with eNOS was also detected in aortic homogenates of high-cholesterol diet–fed Abcg1 −/− mice, paralleling a decrease in eNOS activity and impaired endothelial function. Conclusion— The promotion of cholesterol efflux via ABCG1 results in a reduced inhibitory interaction of eNOS with Cav-1.
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Some other studies have not found an effect of CAV1 on cholesterol efflux, and the overall effect of CAV1 on cholesterol efflux is complex.
“Some other studies have not found an effect6263, and the overall effect of CAV1 on cholesterol efflux is complex61.”
From Prolonged sleep restriction induces changes in pathways involved in cholesterol metabolism and inflammatory responses (2016) · cites this paper as [63]
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Cites
High-Density Lipoprotein — The Clinical Implications of Recent Studies
Desforges, J. F., Gordon, D. J., & Rifkind, B. M. (1989) · New England Journal of Medicine · doi:10.1056/nejm198911093211907 · Cited by 1,547 · see citing works
Okamoto, T., Schlegel, A., Scherer, P. E., & Lisanti, M. P. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.10.5419 · Cited by 1,496 · see citing works
Wang, N., Lan, D., Chen, W., Matsuura, F., & Tall, A. R. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0403506101 · Cited by 1,059 · see citing works
Self-citationCellular and molecular mechanisms of endothelial cell dysfunction.
Harrison, D. G. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119751 · Cited by 1,048 · see citing works
Song, K. S., Li, S. C., Okamoto, T., Quilliam, L. A., Sargiacomo, M., & Lisanti, M. P. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.16.9690 · Cited by 1,000 · see citing works
Zeiher, A. M., Drexler, H., Wollschläger, H., & Just, H. (1991) · Circulation · doi:10.1161/01.cir.83.2.391 · Cited by 994 · see citing works
Oxysterols and atherosclerosis
Brown, A. J., & Jessup, W. (1999) · Atherosclerosis · doi:10.1016/s0021-9150(98)00196-8 · Cited by 921 · see citing works
Kennedy, M. A., Barrera, G. C., Nakamura, K., Baldán, Á., Tarr, P. T., Fishbein, M. C., Frank, J. S., Francone, O. L., & Edwards, P. A. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2005.01.002 · Cited by 885 · see citing works
Endothelial and Antithrombotic Actions of HDL
Mineo, C., Deguchi, H., Griffin, J. H., & Shaul, P. W. (2006) · Circulation Research · doi:10.1161/01.res.0000225982.01988.93 · Cited by 650 · see citing works
HDL Cholesterol and Protective Factors in Atherosclerosis
Assmann, G., & Gotto, A. M. (2004) · Circulation · doi:10.1161/01.cir.0000131512.50667.46 · Cited by 630 · see citing works
Molecular regulation of HDL metabolism and function: implications for novel therapies
Rader, D. J. (2006) · Journal of Clinical Investigation · doi:10.1172/jci30163 · Cited by 603 · see citing works
Zou, M., Shi, C., & Cohen, R. A. (2002) · Journal of Clinical Investigation · doi:10.1172/jci14442 · Cited by 603 · see citing works
Macrophage ABCA1 and ABCG1, but not SR-BI, promote macrophage reverse cholesterol transport in vivo
Wang, X., Collins, H. L., Ranalletta, M., Fuki, I. V., Billheimer, J. T., Rothblat, G. H., Tall, A. R., & Rader, D. J. (2007) · Journal of Clinical Investigation · doi:10.1172/jci32057 · Cited by 593 · see citing works
Self-citationWang, N., Silver, D. L., Costet, P., & Tall, A. R. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m005438200 · Cited by 569 · see citing works
Self-citationBucci, M., Gratton, J., Rudic, R. D., Acevedo, L. M., Roviezzo, F., Cirino, G., & Sessa, W. C. (2000) · Nature Medicine · doi:10.1038/82176 · Cited by 566 · see citing works
Self-citationMalhotra, S. K. (1983) · Cited by 556 · see citing works
HDL, ABC transporters, and cholesterol efflux: implications for the treatment of atherosclerosis
Tall, A. R., Yvan-Charvet, L., Terasaka, N., Pagler, T., & Wang, N. (2008) · Cell Metabolism · doi:10.1016/j.cmet.2008.03.001 · Cited by 540 · see citing works
Self-citationOram, J. F., Lawn, R. M., Garvin, M. R., & Wade, D. P. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m006738200 · Cited by 537 · see citing works
Yvan-Charvet, L., Ranalletta, M., Wang, N., Han, S., Terasaka, N., Li, R., Welch, C., & Tall, A. R. (2007) · Journal of Clinical Investigation · doi:10.1172/jci33372 · Cited by 536 · see citing works
Self-citationBossaller, C., Habib, G. B., Yamamoto, H., Williams, C., Wells, S., & Henry, P. D. (1987) · Journal of Clinical Investigation · doi:10.1172/jci112779 · Cited by 531 · see citing works
Cell cholesterol efflux: integration of old and new observations provides new insights
Rothblat, G. H., de la Llera-Moya, M., Atger, V., Kellner-Weibel, G., Williams, D. L., & Phillips, M. C. (1999) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32113-1 · Cited by 502 · see citing works
Zou, M.-H., Shi, C., & Cohen, R. A. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0214442 · Cited by 494 · see citing works
Féron, O., Dessy, C., Desager, J., & Balligand, J. (2001) · Circulation · doi:10.1161/01.cir.103.1.113 · Cited by 418 · see citing works
Förstermann, U., Mügge, A., Alheid, U., Haverich, A., & Frölich, J. C. (1988) · Circulation Research · doi:10.1161/01.res.62.2.185 · Cited by 418 · see citing works
Féron, O., Dessy, C., Moniotte, S., Desager, J., & Balligand, J. (1999) · Journal of Clinical Investigation · doi:10.1172/jci4829 · Cited by 413 · see citing works
Uittenbogaard, A., Shaul, P. W., Yuhanna, I. S., Blair, A., & Smart, E. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.15.11278 · Cited by 310 · see citing works
Matsuura, F. (2006) · Journal of Clinical Investigation · doi:10.1172/jci27602 · Cited by 293 · see citing works
Bisoendial, R. J., Hovingh, G. K., Levels, J. H. M., Lerch, P. G., Andresen, I., Hayden, M. R., Kastelein, J. J. P., & Stroes, E. S. G. (2003) · Circulation · doi:10.1161/01.cir.0000070934.69310.1a · Cited by 287 · see citing works
Terasaka, N., Wang, N., Yvan‐Charvet, L., & Tall, A. R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0704602104 · Cited by 273 · see citing works
Self-citationVaughan, A. M., & Oram, J. F. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m505368200 · Cited by 266 · see citing works
Zeiher, A. M., Schächlinger, V., Hohnloser, S. H., Saurbier, B., & Just, H. (1994) · Circulation · doi:10.1161/01.cir.89.6.2525 · Cited by 258 · see citing works
Murata, T., Lin, M. I., Huang, Y., Yu, J., Bauer, P. M., Giordano, F. J., & Sessa, W. C. (2007) · The Journal of Experimental Medicine · doi:10.1084/jem.20062340 · Cited by 246 · see citing works
Self-citationDistinction between signaling mechanisms in lipid rafts vs. caveolae
Sowa, G., Pypaert, M., & Sessa, W. C. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.241409998 · Cited by 233 · see citing works
Self-citationABCG1 and HDL protect against endothelial dysfunction in mice fed a high-cholesterol diet
Terasaka, N., Yu, S., Yvan‐Charvet, L., Wang, N., Mzhavia, N., Langlois, R., Pagler, T. A., Li, R., Welch, C. L., Goldberg, I. J., et al. (2008) · Journal of Clinical Investigation · doi:10.1172/jci35470 · Cited by 232 · see citing works
Self-citationKuvin, J. T., Rämet, M. E., Patel, A. R., Pandian, N. G., Mendelsohn, M. E., & Karas, R. H. (2002) · American Heart Journal · doi:10.1067/mhj.2002.123145 · Cited by 231 · see citing works
High-Density Lipoproteins and Endothelial Function
O’Connell, B. J., & Genest, J. J. (2001) · Circulation · doi:10.1161/hc3901.096667 · Cited by 220 · see citing works
Yvan‐Charvet, L., Matsuura, F., Wang, N., Bamberger, M. J., Nguyen, T. T., Rinninger, F., Jiang, X., Shear, C. L., & Tall, A. R. (2007) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.106.138347 · Cited by 200 · see citing works
Self-citationFernandez-Hernando, C. F., Yu, J., Suárez, Y., Rahner, C., Dávalos, A., Lasunción, M. A., & Sessa, W. C. (2009) · Cell Metabolism · doi:10.1016/j.cmet.2009.06.003 · Cited by 192 · see citing works
Self-citationChen, W., Sun, Y., Welch, C. L., Gorelik, A., Leventhal, A. R., Tabas, I. A., & Tall, A. R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m107938200 · Cited by 176 · see citing works
Self-citationMason, R. P., Walter, M. F., & Jacob, R. F. (2004) · Circulation · doi:10.1161/01.cir.0000129503.62747.03 · Cited by 175 · see citing works
Rohrer, L., Hersberger, M., & von Eckardstein, A. (2004) · Current Opinion in Lipidology · doi:10.1097/00041433-200406000-00006 · Cited by 175 · see citing works
Protective effect of high density lipoprotein on endothelium-dependent vasodilatation
Li, X.-P., Zhao, S., Zhang, X., Liu, L., Gao, M., & Zhou, Q. (2000) · International Journal of Cardiology · doi:10.1016/s0167-5273(00)00221-7 · Cited by 133 · see citing works
Cellular Physiology of Cholesterol Efflux in Vascular Endothelial Cells
O’Connell, B. J., Denis, M., & Genest, J. J. (2004) · Circulation · doi:10.1161/01.cir.0000146333.20727.2b · Cited by 95 · see citing works
Relation between high-density lipoprotein cholesterol and peripheral vasomotor function
Kuvin, J. T., Patel, A. R., Sidhu, M. S., Rand, W. M., Sliney, K. A., Pandian, N. G., & Karas, R. H. (2003) · The American Journal of Cardiology · doi:10.1016/s0002-9149(03)00623-4 · Cited by 88 · see citing works
Nitric oxide and hypercholesterolemia: a matter of oxidation and reduction?
Wever, R. M. F., Stroes, E. S. G., & Rabelink, T. J. (1998) · Atherosclerosis · doi:10.1016/s0021-9150(97)00304-3 · Cited by 58 · see citing works
Impairment of Endothelium-Dependent Arterial Relaxation By High-Fat Feeding in ApoE-Deficient Mice
Deckert, V., Lizard, G., Duverger, N., Athias, A., Palleau, V., Emmanuel, F., Moisant, M., Gambert, P., Lallemant, C., & Lagrost, L. (1999) · Circulation · doi:10.1161/01.cir.100.11.1230 · Cited by 40 · see citing works
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Besler, C., Heinrich, K., Rohrer, L., Doerries, C., Riwanto, M., Shih, D. M., Chroni, A., Yonekawa, K., Stein, S., Schaefer, N., et al. (2011) · Journal of Clinical Investigation · doi:10.1172/jci42946 · Cited by 535 · see citing works
Aho, V., Ollila, H. M., Kronholm, E., Bondia-Pons, I., Soininen, P., Kangas, A. J., Hilvo, M., Seppälä, I., Kettunen, J., Oikonen, M., et al. (2016) · Scientific Reports · doi:10.1038/srep24828 · Cited by 126 · see citing works
Gu, H.-M., Wang, F.-Q., & Zhang, D.-W. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2014.02.002 · Cited by 39 · see citing works
- Authors
- Terasaka, N., Westerterp, M., Koetsveld, J., Fernández-Hernando, C., Yvan-Charvet, L., Wang, N., Sessa, W. C., & Tall, A. R.
- Affiliations
- Yale University (US)
- From the Division of Molecular Medicine (N.T., M.W., J.K., L.Y.-C., N.W., and A.R.T.), the Department of Medicine, Columbia University, New York, NY; and the Department of Pharmacology and Vascular Biology and the Therapeutics Program (C.F.-H. and W.C.S.), Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Conn.
- Venue
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Published
- 2010-11
- Volume
- 30
- Issue
- 11
- Pages
- 2219-2225
- ISSN
- 1079-5642 (ISSN-L)
- 1524-4636
- PMC
- PMC4790108
- OpenAlex
- W2105971827
- Cited by
- 107
- References
- 46
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 4 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 9 · 2015: 7 · 2014: 14 · 2013: 19 · 2012: 9
- FWCI
- 12.81
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
- PubMed (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion)
- PubMed Central (acceptedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R01HL061371, R37HL061371)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- ABCG1, high-density lipoprotein, caveolin-1, endothelial nitric oxide synthase, cholesterol efflux, endothelial dysfunction, nitric oxide production, endothelial cells, HDL cholesterol
- MeSH terms
- ATP Binding Cassette Transporter, Subfamily G, Member 1, Animals, Humans, Cholesterol, HDL, Nitric Oxide, ATP-Binding Cassette Transporters, Endothelial Cells, Caveolin 1, Mice, Nitric Oxide Synthase Type III
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