Cited by 100Neuropeptides and Animal PhysiologyAngiogenesis and VEGF in CancerMesenchymal stem cell research

Role for Substance P–Based Nociceptive Signaling in Progenitor Cell Activation and Angiogenesis During Ischemia in Mice and in Human Subjects

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Amadesi, Silvia, Reni, Carlotta, Katare, Rajesh, Meloni, Marco, Oikawa, Atsuhiko, Beltrami, Antonio P., Avolio, Elisa, Cesselli, Daniela +8 more·2012·Circulation

Abstract

Background—: Pain triggers a homeostatic alarm reaction to injury. It remains unknown, however, whether nociceptive signaling activated by ischemia is relevant for progenitor cells (PC) release from bone marrow. To this end, we investigated the role of the neuropeptide substance P (SP) and cognate neurokinin 1 (NK1) nociceptor in PC activation and angiogenesis during ischemia in mice and in human subjects. Methods and Results—: The mouse bone marrow contains sensory fibers and PC that express SP. Moreover, SP-induced migration provides enrichment for PC that express NK1 and promote reparative angiogenesis after transplantation in a mouse model of limb ischemia. Acute myocardial infarction and limb ischemia increase SP levels in peripheral blood, decrease SP levels in bone marrow, and stimulate the mobilization of NK1-expressing PC, with these effects being abrogated by systemic administration of the opioid receptor agonist morphine. Moreover, bone marrow reconstitution with NK1-knockout cells results in depressed PC mobilization, delayed blood flow recovery, and reduced neovascularization after ischemia. We next asked whether SP is instrumental to PC mobilization and homing in patients with ischemia. Human PC express NK1, and SP-induced migration provides enrichment for proangiogenic PC. Patients with acute myocardial infarction show high circulating levels of SP and NK1-positive cells that coexpress PC antigens, such as CD34, KDR, and CXCR4. Moreover, NK1-expressing PC are abundant in infarcted hearts but not in hearts that developed an infarct after transplantation. Conclusions—: Our data highlight the role of SP in reparative neovascularization. Nociceptive signaling may represent a novel target of regenerative medicine.

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Authors
Amadesi, S., Reni, C., Katare, R., Meloni, M., Oikawa, A., Beltrami, A. P., Avolio, E., Cesselli, D., Fortunato, O., Spinetti, G., Ascione, R., Cangiano, E., Valgimigli, M., Hunt, S. P., Emanueli, C., & Madeddu, P.
Affiliations
University of Udine (IT)
Venue
Circulation
Published
2012-04-10
Volume
125
Issue
14
Pages
1774-1786
ISSN
  • 0009-7322 (ISSN-L)
  • 1524-4539
OpenAlex
W2053495327
Cited by
100
References
45
Citations by year
2025: 4 · 2024: 4 · 2023: 6 · 2022: 5 · 2021: 6 · 2020: 7 · 2019: 9 · 2018: 7 · 2017: 10 · 2016: 13 · 2015: 10 · 2014: 7 · 2013: 10 · 2012: 2
FWCI
3.82
Type
journal article
Language
en
Open access
Locations
  • Circulation (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • CentAUR (University of Reading) (submittedVersion)
  • Institutional Research Information System (University of Udine) (submittedVersion)
  • ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
  • UCL Discovery (University College London) (submittedVersion)
  • Explore Bristol Research (submittedVersion)
Funders
  • British Heart Foundation (FS/10/001/27959, PG/09/099/28122)
  • University of Bristol
  • Medical Research Council (MR/J002593/1)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
substance P, neurokinin-1 receptor, nociceptive signaling, progenitor cell mobilization, angiogenesis, ischemia, acute myocardial infarction, limb ischemia, bone marrow, neovascularization, morphine, regenerative medicine, CXCR4, CD34, KDR
MeSH terms
Animals, Humans, Ischemia, Male, Mice, Inbred C57BL, Stem Cells, Substance P, Signal Transduction, Receptors, Calcitonin Gene-Related Peptide, Receptors, Neurokinin-1, Neovascularization, Physiologic, Hematopoietic Stem Cell Mobilization, Mice, Nociception

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