Cited by 222Glaucoma and retinal disordersNerve injury and regenerationRetinal Diseases and Treatments

Effect of CNTF on retinal ganglion cell survival in experimental glaucoma

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Pease, Mary Ellen, Zack, Donald J., Berlinicke, Cynthia, Bloom, Kristen, Cone, Frances, Wang, Yuxia, Klein, Ronald L., Hauswirth, William W. +1 more·2009·Investigative Opthalmology & Visual Science

Abstract

PURPOSE: To assess the neuroprotective effect of virally mediated overexpression of ciliary-derived neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) in experimental rat glaucoma. METHODS: Laser-induced glaucoma was produced in one eye of 224 Wistar rats after injection of adenoassociated viral vectors (type 2) containing either CNTF, BDNF, or both, with saline-injected eyes and noninjected glaucomatous eyes serving as the control. IOP was measured with a hand-held tonometer, and semiautomated optic nerve axon counts were performed by masked observers. IOP exposure over time was adjusted in multivariate regression analysis to calculate the effect of CNTF and BDNF. RESULTS: By multivariate regression, CNTF had a significant protective effect, with 15% less RGC axon death (P < 0.01). Both combined CNTF-BDNF and BDNF overexpression alone had no statistically significant improvement in RGC axon survival. By Western blot, there was a quantitative increase in CNTF and BDNF expression in retinas exposed to single viral vectors carrying each gene, but no increase with sequential injection of both vectors. CONCLUSIONS: These data confirm that CNTF can exert a protective effect in experimental glaucoma. The reason for the lack of observed effect in the BDNF overexpression groups is unclear, but it may be a function of the level of neurotrophin expression achieved.

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Crossref · 10.1167/iovs.08-3013

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  • AAV delivery of BDNF and its receptor or CNTF promoted survival and function of retinal ganglion cells in models of optic nerve crush or retinal ganglion cell degeneration in a model for experimental glaucoma, respectively.

    “Supporting the promise of this method, AAV delivery of BDNF and its receptor105 or CNTF106 promoted survival and function of retinal ganglion cells in models of optic nerve crush or retinal ganglion cell degeneration in a model for experimental glaucoma, respectively.”

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Research works printed but not linked: 1

  1. [69]Cui Q Lu Q So K-F Yip HK CNTF, not other trophic factors, promotes axonal regeneration of axotomized retinal ganglion cells in adult hamsters Invest Ophthalmol Vis Sci 1999 40 760 766 10067981

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Authors
Pease, M. E., Zack, D. J., Berlinicke, C., Bloom, K., Cone, F., Wang, Y., Klein, R. L., Hauswirth, W. W., & Quigley, H. A.
Affiliations
  • From the Departments of Ophthalmology,
  • From the Departments of Ophthalmology,2Molecular Biology and Genetics, and3Neuroscience, the4Institute of Genetic Medicine, and
  • Louisiana State University Health Sciences Center Shreveport (US)
  • University of Florida (US)
  • Johns Hopkins University (US)
Venue
Investigative Opthalmology & Visual Science
Published
2009-05-01
Volume
50
Issue
5
Pages
2194
ISSN
  • 0146-0404 (ISSN-L)
  • 1552-5783
OpenAlex
W2094688362
Cited by
222
References
88
Citations by year
2026: 2 · 2025: 11 · 2024: 11 · 2023: 15 · 2022: 8 · 2021: 13 · 2020: 19 · 2019: 17 · 2018: 15 · 2017: 8 · 2016: 19 · 2015: 13 · 2014: 10 · 2013: 13 · 2012: 9
FWCI
15.92
Type
journal article
Language
en
Open access
Locations
  • Investigative Ophthalmology & Visual Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Johns Hopkins University
  • National Eye Institute (EY 13729, P30EY021721, R56EY002120, EY 08571, U10EY013729, #EY01765, EY 02120, P30EY008571, P30EY001765, R01EY011123, EY-11123, R01EY002120)
Topics
  • Health Sciences › Medicine › Ophthalmology › Glaucoma and retinal disorders
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
Keywords
ciliary neurotrophic factor, brain-derived neurotrophic factor, retinal ganglion cell survival, experimental glaucoma, laser-induced glaucoma, neuroprotection, adeno-associated virus vectors, gene overexpression, intraocular pressure, neurotrophins
MeSH terms
Dependovirus, Animals, Axons, Cell Count, Cell Survival, Disease Models, Animal, Gene Expression Regulation, Genetic Vectors, Glaucoma, Intraocular Pressure, Male, Optic Nerve Diseases, Plasmids, Retinal Ganglion Cells, Tonometry, Ocular, Blotting, Western, Genetic Therapy, Rats, Wistar, Brain-Derived Neurotrophic Factor, Cytoprotection, Ciliary Neurotrophic Factor, Rats

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