Volumetric measurement of subretinal blebs using microscope-integrated optical coherence tomography
Open accessAbstract
Purpose: We advance studies of subretinal treatments by developing a microscope-integrated optical coherence tomography (MIOCT) image-based method for measuring the volume of therapeutics delivered into the subretinal space. Methods: A MIOCT image-based volume measurement method was developed and assessed for accuracy and reproducibility by imaging an object of known size in model eyes. This method then was applied to subretinal blebs created by injection of diluted triamcinolone. Bleb volumes obtained from MIOCT were compared to the intended injection volume and the surgeon's estimation of leakage. Results: Validation of the image-based volume measurement method showed accuracy to ±1.0 μL (6.0% of measured volume) with no statistically significant variation under different imaging settings. When this method was applied to subretinal blebs, four of 11 blebs without surgeon-observed leakage yielded a mean volume of 32 ± 12.5 μL, in contrast to the intended 50 μL volume injected from the delivery device. This constituted a mean difference of −18 μL (mean percent error, 36 ± 25%). For all 11 blebs, the surgeon's estimations of leakage were significantly different from and showed no correlation with the volume loss based on image-based volume measurements (P < 0.001, paired t-test; intraclass correlation = 0). Conclusions: We validated an accurate and reproducible method for measuring subretinal volumes using MIOCT. Use of this method revealed that the intended volume might not be delivered into the subretinal space. MIOCT can allow for accurate assessment of subretinal dose delivered, which may have therapeutic implications in evaluating the efficacy and toxicity of subretinal therapies. Translational Relevance: Use of MIOCT can provide feedback on the accuracy of subretinal injection volumes delivered.
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Direct volumetric measurements using intraoperative OCT showed that subretinal bleb size was on average 36% smaller than predicted by the surgeon using a dilute triamcinolone solution.
“Direct volumetric measurements using intraoperative OCT showed that subretinal bleb size was on average 36% smaller than predicted by the surgeon using a dilute triamcinolone solution (23).”
From Subretinal therapy: technological solutions to surgical and immunological challenges (2022) · cites this paper as [23]
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- Authors
- Hsu, S. T., Gabr, H., Viehland, C., Sleiman, K., Ngo, H. T., Carrasco-Zevallos, O. M., Vajzovic, L., McNabb, R. P., Stinnett, S. S., Izatt, J. A., Kuo, A. N., & Toth, C. A.
- Affiliations
- Duke University (US)
- Ain Shams University (EG)
- Department of Biomedical Engineering, Duke University, Durham, NC, USA
- Corresponding author
- Toth, Cynthia A.
- ORCID
- Venue
- Translational Vision Science & Technology
- Published
- 2018-04-05
- Volume
- 7
- Issue
- 2
- Pages
- 19
- ISSN
- 2164-2591 (ISSN-L)
- PMC
- PMC5894912
- OpenAlex
- W2795968500
- Cited by
- 36
- References
- 32
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 8 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 8 · 2019: 2 · 2018: 1
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Translational Vision Science & Technology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Eye Institute (R01EY023039)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Optical Coherence Tomography Applications
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Retinal Imaging and Analysis
- Keywords
- subretinal injection, triamcinolone, bleb leak
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